Labeling of whole-mount retina and retinal cryo-sections were done as previously described [11 (link),16 (link)]. Primary antibodies used were gp130 (1:200; Cat# MAB4681, R&D Systems) and β-Tubulin III (TUJ1, 1:500; Cat#845501; BioLegend). Secondary antibodies were used at a concentration of 1:200 and consisted of donkey α-rat 488 (cat# 712-545-150; Jackson Immuno) or donkey α-rabbit 647 (cat# 711-606-152; Jackson Immuno). Z-stack images (0.5 μm/stack) were obtained on an inverted confocal microscope (Olympus) at the Vanderbilt University Cell Imaging Services Core and were analyzed with FV-10 ASW image analysis software (Olympus).
Mab4681
Manufactured by R&D Systems
MAB4681 is a Mouse Monoclonal Antibody produced in vitro. It is designed for use in Immunohistochemistry (IHC) and Western Blot applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mab4681
1
Retinal Immunohistochemistry for Confocal Imaging
2
Retinal Immunohistochemistry for Confocal Imaging
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