Cck 8 detection solution

In 96-well plates, 3 × 10³ Huh7 and MHCC97H cells were cultivated to determine cell proliferation. Following 24, 48, and 72 h of growth, 10 µL CCK-8 detection solution (cat# 40203ES80, Yeasen Biotechnology) was added to each well, and the absorbance was recorded at 450 nm after 2 h of incubation.

Before undergoing CCK-8 assay, the NRVMs were washed three times with HBSS. Fresh culture medium (high-glucose DMEM containing 0.5% FBS and 1% penicillin–streptomycin) and CCK-8 detection solution (Yeasen, 40203ES76) were applied to each well according to the manufacturer's instructions, and the cells were cultured for another 2.5 h. The CCK-8 value was measured at 450 nm absorbance. Regarding NRVMs cultured in 24-well plates, the supernatants (50 μL/well) were transferred to a 96-well plate after cells were incubated with detection solution, and the CCK-8 value was then assessed.