Anti p27

Manufactured by Bioworld Technology

Sourced in United States

Anti-p27 is a laboratory reagent used for the detection and quantification of the p27 protein in biological samples. It is a specific antibody that can bind to the p27 protein, allowing for its identification and measurement through various analytical techniques.

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4 protocols using anti p27

Molecular Mechanisms Underlying Autophagy Regulation

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Spautin-1(S7888), 3-methyladanine (3-MA, S2767), Z-VAD-FMK (S7023), SP600125 (S1460), SB230580 (S1076), LY3214996 (S8534), Gefitinib (S1025) and Enzalutamide (MDV3100, S1250) were purchased from Sellectchem (Houston, TX, USA). SKP2-C25 (M60136) was obtained from Xcessbio Biosciences, Inc. (San Diego, CA). USP10 (sc-76,811), USP13 (sc-76,815) and Glut1 (sc-35,493) siRNAs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies: anti-Glut1 (ab652), anti-USP13 (ab109264) (Abcam, Cambridge, MA); anti-GAPDH (BS60630), anti-Ki67 (BS1454) (Bioworld Technology, Inc., Louis Park, MN, USA); anti-USP10(#8501), anti-SKP2(#2652), anti-p27(#3686), anti-CDK4(#12790), anti-CDK2(#2546), anti-CyclinD1(#2978), anti-p15(#4822), anti-p21(#2947), anti-PARP(#9532), anti-Bim(#2933), anti-Bax(#14796), anti-Bcl-2(#15071), anti-activated Caspase-3(#9664), anti-phospho-JNK (#9255), anti-JNK(#9252), anti-phospho-ERK1/2(#4370), anti-ERK1/2(#4695), anti-phospho-p38(#4511), anti-p38(#8690), anti-phospho-MKK4(#4514), anti-MKK4(#9152), anti-phospho-MEK1/2 (#9154), anti-MEK1/2(#4694), anti-phospho-EGFR (Tyr1173)(#4407), anti-phospho-EGFR (Tyr1068)(#3777), anti-EGFR(#2085) (Cell Signaling Technology, Beverly, MA, USA).

Liao Y., Guo Z., Xia X., Liu Y., Huang C., Jiang L., Wang X., Liu J, & Huang H. (2019). Inhibition of EGFR signaling with Spautin-1 represents a novel therapeutics for prostate cancer. Journal of Experimental & Clinical Cancer Research : CR, 38, 157.

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Western Blot Analysis of Protein Expression

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We carried out western blot as previously described [33 (link)], using anti-TLE3 (Abcam, Cambridge, MA, USA), anti-FOXO3, anti-Akt, anti-GSK-3β, anti-ERK, anti-p21, anti-p27, anti-p-FOXO3, anti-p-Akt, anti-p-GSK-3β and anti-p-ERK (Bioworld Technology, St. Louis Park, MN, USA) to detect the corresponding proteins. Anti-α-Tubulin monoclonal antibody (Sigma, St. Louis, MO, USA) served as a loading control.

Yang R.W., Zeng Y.Y., Wei W.T., Cui Y.M., Sun H.Y., Cai Y.L., Nian X.X., Hu Y.T., Quan Y.P., Jiang S.L., Wang M., Zhao Y.L., Qiu J.F., Li M.X., Zhang J.H., He M.R., Liang L., Ding Y.Q, & Liao W.T. (2016). TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways. Journal of Experimental & Clinical Cancer Research : CR, 35, 152.

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Protein Expression Analysis by Western Blot

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Proteins were isolated, subjected to SDS-page, transferred onto PVDF membranes and incubated with antibodies anti-SIAH1 (Abcam, Cambridge, MA, USA), anti-SFRP2 (Cell Signaling Technology, Danvers, MA, USA), anti-KRAS (Proteintech, USA), anti-Ki-67 (Abcam, Cambridge, MA, USA), anti-p27, anti-p21, anti-cyclinD1 (Bioworld Technology Inc. St. Louis Park, MN, USA). LamB1 and a-tubulin (Sigma, Saint Louis, MO, USA) were used as loading controls. Then, the membranes were detected by chemiluminescence. All operations above were performed in accordance with standard methods.

Ye Y.P., Wu P., Gu C.C., Deng D.L., Jiao H.L., Li T.T., Wang S.Y., Wang Y.X., Xiao Z.Y., Wei W.T., Chen Y.R., Qiu J.F., Yang R.W., Lin J., Liang L., Liao W.T, & Ding Y.Q. (2016). miR-450b-5p induced by oncogenic KRAS is required for colorectal cancer progression. Oncotarget, 7(38), 61312-61324.

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Western Blotting Characterization of Signaling Proteins

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We carried out western blotting according to standard methods, which protein lysates were prepared, subjected to SDS–PAGE and transferred onto PVDF membranes, using anti-AKT (Cell Signaling Technology, Danvers, MA, USA), anti-ERK1/2 (CST, USA), anti-GSK-3β (CST, USA), anti-p21, anti-p27, anti-CyclinD1 (Bioworld Technology, St. Louis Park, MN, USA), anti-p-AKT (CST, USA), anti-p-GSK-3βand anti-p-ERK1/2 (CST, USA). Anti-α-Tubulin (Sigma, St. Louis, MO, USA) monoclonal antibody served as a internal reference.

Wei W.T., Nian X.X., Wang S.Y., Jiao H.L., Wang Y.X., Xiao Z.Y., Yang R.W., Ding Y.Q., Ye Y.P, & Liao W.T. (2017). miR-422a inhibits cell proliferation in colorectal cancer by targeting AKT1 and MAPK1. Cancer Cell International, 17, 91.

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