Tds 1002

Manufactured by Tektronix

Sourced in United States

The TDS 1002 is a digital storage oscilloscope manufactured by Tektronix. It is a compact and portable instrument designed for basic waveform analysis. The TDS 1002 features a 60 MHz bandwidth, 1 GS/s sample rate, and 2-channel input. It provides digital storage and display of acquired waveforms, allowing users to analyze and measure electrical signals.

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Lab products found in correlation

Afg3022b, by Tektronix (3 mentions) Celesta light engine, by Lumencor (1 mentions) Cascade blue, by Thermo Fisher Scientific (1 mentions) Prime 95b, by Teledyne (1 mentions) Eclipse ti2, by Nikon (1 mentions) Smz800 microscope, by Nikon (1 mentions)

5 protocols using tds 1002

Acoustophoresis Imaging of Blood Cells

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To visualize the acoustophoretic motion of cells, the chip was mounted upside down in an inverted microscope (Eclipse Ti2, Nikon, Tokyo, Japan) equipped with a CMOS camera (Prime 95B, Teledyne Photometrics, Tucson, Arizona). Stained PBMCs and neutrophils were spiked into the whole blood and imaged at the end of the separation channel. To monitor the density-adjusted medium at the channel outlet, we added a fluorescent tracer molecule (Dextran, Cascade Blue, 3000 MW, Thermo Fisher Scientific) under the assumption that iodixanol and dextran molecules diffuse at the same rate (D_iodixanol ≈ 2.5 × 10^–10 m² s⁻¹ and D_dextran ≈ 2.2 × 10^–10 m² s⁻¹). A laser illumination unit (Celesta light engine, Lumencor, OR, USA) was used with a multiband filter set (CELESTA-DA/FI/TR/Cy5/Cy7-A-000, Semrock optical filters, IDEX Health & Science) in three excitation channels with peak wavelengths at 365 nm, 488 nm, and 561 nm. The excitation channels and the camera were activated through external triggering.
When operating the chip outside of the AcouWash system the PZT transducer was driven by a function generator (AFG3022B, Tektronix, Inc., Beaverton, Oregon, USA) to deliver a resonant frequency (1.99 MHz) and different applied voltages as measured over the piezo with an oscilloscope (TDS1002, Tektronix, Inc., Beaverton, Oregon, USA).

Alsved J., Rezayati Charan M., Ohlsson P., Urbansky A, & Augustsson P. (2024). Label-free separation of peripheral blood mononuclear cells from whole blood by gradient acoustic focusing. Scientific Reports, 14, 8748.

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Acoustophoretic System Setup and Characterization

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The standing wave field was created using piezoceramic transducers glued underneath the pre-focusing channel as well as underneath the main separation channel. Frequencies were set to 4.831 MHz for the pre-focusing channel with 5 V_pp amplitude and 1.956 MHz for the main separation channel. A dual channel function generator (AFG3022B, Tektronix, Beaverton, OR, USA), connected to signal amplifiers (in-house build) was used to drive both transducers while the voltage over each transducer was measured via a two-channel digital oscilloscope (TDS 1002, Tektronix). Temperature regulation was achieved using a Peltier element and a PT100 resistance temperature detector attached to the acoustophoretic system.

Urbansky A., Ohlsson P., Lenshof A., Garofalo F., Scheding S, & Laurell T. (2017). Rapid and effective enrichment of mononuclear cells from blood using acoustophoresis. Scientific Reports, 7, 17161.

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Acoustophoresis Chip Design and Fabrication

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A detailed description of the acoustophoresis chip design and fabrication process can be found in Augustsson et al. [26 (link)]. In brief, the structure of the microchannel was made by anisotropic wet etching in a silicon wafer using standard photolithography and anisotropic KOH etching, and sealed with a glass lid using anodic bonding. The chip consists of a sample inlet, leading to a pre-focusing channel (10 mm × 300 μm × 150 μm), a flow splitter that directs the flow to each side of a central fluid inlet, and a main separation channel (20 mm × 375 μm × 150 μm) ending in a trifurcation with one central outlet and a common outlet for the two side branches (Figure 1). Docking ports for fluidic tubing were glued to the inlets and outlets in form of silicon tubing with an inner diameter of one-sixteenth of an inch.
A piezoceramic transducer resonant at 5 MHz was glued underneath the pre-focusing channel, while a second piezoceramic transducer resonant at 2 MHz was attached underneath the main separation channel. Both transducers were driven by a dual channel function generator (AFG3022B, Tektronix, Beaverton, OR, USA), equipped with signal amplifiers (in-house build), and the voltage over each transducer was measured via a two-channel digital oscilloscope (TDS 1002, Tektronix). For visualization of the separation procedure a Nikon SMZ800 microscope (Nikon, Tokyo, Japan) was used.

Urbansky A., Lenshof A., Dykes J., Laurell T, & Scheding S. (2016). Affinity-Bead-Mediated Enrichment of CD8+ Lymphocytes from Peripheral Blood Progenitor Cell Products Using Acoustophoresis. Micromachines, 7(6), 101.

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Ultrasonic Characterization of Material Properties

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The Poisson’s ratio (V) and elastic modulus
(E) were determined by the ultrasonic pulse-echo method
[37 ] using a 200 MHz ultrasonic
pulser-receiver (5900 PR; Panametrics, Waltham, MA, USA), 20 MHz longitudinal
and shear transducers with a delay material, and a coupling paste (Panametrics)
applied between the specimen and transducer. The time of flight of ultrasonic
pulse was measured with an oscilloscope (TDS 1002; Tektronix, Shanghai, China)
and the thickness of the specimen was measured with a digital micrometer
(Mitutoyo). Sonic velocities were calculated as two times the thickness divided
by the time of flight, since in the pulse-echo method only one transducer
(longitudinal or shear mode) was used to emit and capture the back-reflected
wave. V and E values were calculated using the
equations:

V = 0.5 (\frac{V_{l}^{2} - 2 V_{t}^{2}}{V_{I}^{2} - V_{t}^{2}})

E = ρ (\frac{3 V_{t}^{2} V_{I}^{2} - 4 V_{t}^{4}}{V_{I}^{2} - V_{t}^{2}})

where ρ is density measured by
Archimedes principle, V_l is longitudinal velocity, and V_tis shear velocity.

Kaizer M.R., Gonçalves A.P., Soares P.B., Zhang Y., Cesar P.F., Cava S.S, & Moraes R.R. (2016). Mono or polycrystalline alumina-modified hybrid ceramic biomaterials. Dental materials : official publication of the Academy of Dental Materials, 32(3), 450-460.

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Ultrasound Pressure Mapping in Reactor

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Sound Pressure Levels (SPL) during full power operation at 0.4 and 2 MHz frequency (Fig. 3a) were measured in water and paste using a hydrophone device (Fig. 3a) encompassing a needle hydrophone (HNR_1000 S/N: 1761, Onda, Sunnyvale, CA, United States of America, external amplifier Onda AH2010 S/N: 1589; Nom. Gain: 20 dB) with a protective glass membrane for direct measurement on paste. Hydrophones are very sensitive to the presence of oil and protein material and therefore manufacturers do not recommend direct contact with food materials. Although hydrophones have a directive response, they give qualitative information about the pressure field inside the reactor.
Sound measurements were carried out at twelve specific points in the reactor as shown in Fig. 3b. In the experiment 2, the measurements were carried out at two transducers to wall distances (9 and 14.5 cm), and two different depths into the vessel (close to top and bottom). This information gives a qualitative sound distribution map across the reactor (Fig. 3b). The peak-to-peak voltages were recorded three times at each measurement point with an oscilloscope (TDS 1002, Tektronix, Beaverton, Oregon, United States of America) and the mean value was converted into sound pressure level (SPL) using Eq. (1). Trials were carried out at room temperature with a maximum sonication time of 5 min.

Amarillo M., Pérez N., Blasina F., Gambaro A., Leone A., Romaniello R., Xu X.Q, & Juliano P. (2019). Impact of sound attenuation on ultrasound-driven yield improvements during olive oil extraction. Ultrasonics sonochemistry, 53.

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