Minocycline

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

Minocycline is a broad-spectrum tetracycline antibiotic. It is used as a laboratory reagent for research and development purposes.

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Lab products found in correlation

8 protocols using minocycline

Antimicrobial Susceptibility Evaluation

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The disk diffusion method was used to evaluate susceptibility to the following antimicrobial agents: imipenem (IPM: 10 μg), ceftazidime (CAZ: 30 μg), amikacin (AMK: 30 μg), piperacillin/tazobactam (TZP: 100/10 μg), levofloxacin (LVX: 5 μg), ticarcillin/Clavulanic acid (TCC: 75/10 μg), minocycline (MNO: 30 μg) (Oxoid, UK). Results were interpreted in accordance with CLSI guidelines from 2011. Isolates with intermediate susceptibility were classified as non-susceptible.

Ning N.Z., Liu X., Bao C.M., Chen S.M., Cui E.B., zhang J.L., Huang J., Chen F.H., Li T., Qu F, & Wang H. (2017). Molecular epidemiology of blaOXA-23-producing carbapenem-resistant Acinetobacter baumannii in a single institution over a 65-month period in north China. BMC Infectious Diseases, 17, 14.

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Antimicrobial Susceptibility of B. multivorans Strain

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Strain AS149, a B. multivorans isolated from the sputum of a cystic fibrosis patient [11 (link)], as the ancestral parent. Bacteria were grown at 37° on LB for routine culturing and during experimental evolution and on Mueller-Hinton broth 2 (Sigma-Aldrich) for antimicrobial susceptibility testing.
Antibiotics involved in this study were chosen due to their inclusion in the CLSI [12 ] list of standard antibiotics tested against B. cepacia and for having varied targets, which we separated in βLA (meropenem [MEM], ceftazidime [CAZ]) and non-βLA (chloramphenicol [CHL], levofloxacin [LVX], minocycline [MIN], trimethoprim- sulfamethoxazole [SXT]) groups. BBL Sensi-Disc antimicrobial susceptibility test disks (BD) were used for all except minocycline (Oxoid). The minimum inhibitory concentration was determined using ETEST gradient strips.

Kavanaugh L.G., Flanagan J.N, & Steck T.R. (2020). Reciprocal antibiotic collateral sensitivity in Burkholderia multivorans. International journal of antimicrobial agents, 56(1), 105994.

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Susceptibility Testing and Resistance Genes in Acinetobacter

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Susceptibility testing was performed using the MicroScan NM31 microdilution method (Dade Behring, West Sacramento, CA, USA). Disc diffusion was used to determine susceptibility to doxycycline, minocycline and colistin (all from Oxoid, Basingstoke, Hants, UK). Imipenem susceptibility was confirmed using E-test strips (bioMérieux, Marcy-l’Etoile, France). Results were interpreted using the Clinical Laboratory Standards Institute criteria for Acinetobacter spp. The control strains used were E. coli ATCC 25922 and E. coli ATCC 35218.
Screening for the ADC genes [28 (link)], and the four CHO genes [29 (link)] bla_OXA-51-like, bla_OXA-23-like, bla_OXA-40-like, and bla_OXA-58-like, as well as their most commonly associated ISs (ISAba1, ISAba2, ISAba3, ISAba4 and IS18), was performed. Screening was also undertaken for the MBL genes [30 (link)] bla_IMP, bla_VIM, bla_SIM-1, bla_GIM-1, bla_SPM-1 and bla_NDM-1 [31 (link)]. The PCR methods used were those described in previous work [23 (link)].

Villalón P., Valdezate S., Cabezas T., Ortega M., Garrido N., Vindel A., Medina-Pascual M.J, & Saez-Nieto J.A. (2015). Endemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital. BMC Microbiology, 15, 47.

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Antibiotic Susceptibility of S. maltophilia

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We collected a total of 93 nonrepetitive isolates of S. maltophilia from outpatients and inpatients from Renji Hospital in 2014, in addition to the patients’ clinical information. We identified these isolates using the VITEK-2 Compact System (bioMérieux, Marcy-l’Étoile, France) and confirmed them using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS, Bruker Daltonics, Bremen, Germany). The samples were then stored at minus 80 °C.
We grew the bacteria in tryptic soy broth (TSB; Oxoid, Hampshire, UK) at 37 °C overnight. The antibiotics used for disk diffusion testing were levofloxacin, TMP/SMX, and minocycline (Oxoid). The quality control isolates included Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC27853.

Duan Z., Qin J., Liu Y., Li C, & Ying C. (2020). Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital. BMC Microbiology, 20, 294.

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Antimicrobial Susceptibility Testing of Acinetobacter baumannii

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Antimicrobial susceptibility testing of the isolated strains was performed according to the Clinical and Laboratory Standards Institute guidelines (CLSI, 2016) using the disc diffusion method. We tested the susceptibility of the isolates to piperacillin, ampicillin, ampicillin-sulbactam, piperacillin-tazobactam, ticarcillin-clavulanic acid, ceftazidime, cefepime, cefotaxime, ceftriaxone, imipenem, meropenem, gentamicin, tobramycin, amikacin, minocycline, ciprofloxacin, levofloxacin, and polymyxin B discs (Oxoid, UK). The drug susceptibility data for the A. baumannii isolates were analyzed using WHONET 5.6. Multi-drug resistance for A. baumannii isolates was defined as described previously.1 (link)

Tang J., Chen Y., Wang X., Ding Y., Sun X, & Ni Z. (2020). Contribution of the AbaI/AbaR Quorum Sensing System to Resistance and Virulence of Acinetobacter baumannii Clinical Strains. Infection and Drug Resistance, 13, 4273-4281.

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Antibiotic Susceptibility Testing Protocol

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Mueller-Hinton (MH) agar medium was purchased from Guangzhou Dijing Microbiology Co., Ltd.. Drug sensitivity test paper (combining sulfamethoxazole, SXT; minocycline, MH; levofloxacin, LVX; ticarcillin/clavulanic acid, TIM; ceftazidime, CAZ; chloramphenicol, CL and imipenem, IPM) was purchased from OXOID (United Kingdom).

Liu B, & Tong S. (2019). An investigation of Stenotrophomonas maltophilia-positive culture caused by fiberoptic bronchoscope contamination. BMC Infectious Diseases, 19, 1072.

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Antimicrobial Resistance Profiling of S. maltophilia

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A total of 93 non-repetitive isolates of S. maltophilia from outpatients and inpatients from Renji hospital in 2014 were collected, in addition to the patients' clinical information. These isolates were identified using the VITEK-2 Compact System (bioMérieux, France) and confirmed using matrix-assisted laser desorption ionization time-offlight mass spectrometry (MALDI-TOF MS, Bruker Daltonics, Bremen, Germany). The samples were then stored at minus 80°C.
The bacteria were then grown in tryptic soy broth (TSB; Oxoid) at 37°C overnight. The antibiotics used for disk diffusion testing were levofloxacin, TMP/SMX, and minocycline (Oxoid, UK). The quality control isolates included Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC27853.

Duan Z., Qin J., Liu Y., Li C., & Ying C. (2020). Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital.

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Antibiotic Resistance Profiling of S. maltophilia

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A total of 93 non-repetitive strains of S. maltophilia isolated from outpatients and inpatients from Renji hospital in 2014 were collected, in addition to the patients' clinical information. These strains were identified using the VITEK-2 Compact System (bioMérieux, France) and confirmed using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS, Bruker Daltonics, Bremen, Germany). The samples were then stored at 80°C.
The bacteria were then grown in tryptic soy broth (TSB; Oxoid) at 37°C overnight. The antibiotics used for disk diffusion testing were levofloxacin, trimethoprim-sulfamethoxazole, and minocycline (Oxoid, UK). The quality control strains included Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC27853.

Duan Z., Qin J., Liu Y., Li C., & Ying C. (2020). Molecular epidemiology and risk factors of Stenotrophomonas maltophilia infections in a Chinese teaching hospital.

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