Pierce agarose chip assay kit

Manufactured by Thermo Fisher Scientific

The Pierce Agarose ChIP Assay Kit is a kit designed for chromatin immunoprecipitation (ChIP) experiments. It provides pre-optimized reagents and protocols to aid in the isolation and analysis of protein-DNA complexes from cells or tissues.

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Lab products found in correlation

Imprint rna immunoprecipitation kit, by Merck Group (1 mentions)

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Agarose (403 citations) Pierce Agarose ChIP Kit (249 citations) ChIP assay kit (54 citations) ChIP kit (45 citations) Agarose ChIP Kit (15 citations) Pierce ChIP Kit (3 citations) ChIP) assay (3 citations)

2 protocols using pierce agarose chip assay kit

ChIP-Seq and RIP Protocols

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For chromatin immunoprecipitation (ChIP) experiments, the Pierce Agarose ChIP Assay Kit (Thermo Fisher Scientific) was used. RNA immunoprecipitation (RIP) was performed using Imprint® RNA Immunoprecipitation Kit (Sigma Aldrich); see Supplementary Methods for protocols.

Amodio N., Stamato M.A., Juli G., Morelli E., Fulciniti M., Manzoni M., Taiana E., Agnelli L., Cantafio M.E., Romeo E., Raimondi L., Caracciolo D., Zuccalà V., Rossi M., Neri A., Munshi N.C., Tagliaferri P, & Tassone P. (2018). Drugging the lncRNA MALAT1 via LNA gapmeR ASO inhibits gene expression of proteasome subunits and triggers anti-multiple myeloma activity. Leukemia, 32(9), 1948-1957.

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ChIP-qPCR Assay for SRF Binding

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ChIP assays were performed using the Pierce^™ Agarose ChIP Assay Kit (Thermo Scientific, Catelog # PI26156) according to manufacture instruction. The cross-linked chromatin from subconfluent PAC1 cells in 10% FBS was immunoprecipitated using anti-SRF antibodies (Santa Cruz, sc-335), followed by qPCR using primers specific to the Nik promoter in a region containing the CArG box (Forward primer: 5′—TGTTCAGCCCATTTTTAGGC -3′, Reverse primer: 5′- TTTAGCATTGTGCGAGTGTC -3′) (S2 Table).

Dai X., Thiagarajan D., Fang J., Shen J., Annam N.P., Yang Z., Jiang H., Ju D., Xie Y., Zhang K., Tseng Y.Y., Yang Z., Rishi A.K., Li H.J., Yang M, & Li L. (2017). SM22α suppresses cytokine-induced inflammation and the transcription of NF-κB inducing kinase (Nik) by modulating SRF transcriptional activity in vascular smooth muscle cells. PLoS ONE, 12(12), e0190191.

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