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Pierce agarose chip assay kit

Manufactured by Thermo Fisher Scientific

The Pierce Agarose ChIP Assay Kit is a kit designed for chromatin immunoprecipitation (ChIP) experiments. It provides pre-optimized reagents and protocols to aid in the isolation and analysis of protein-DNA complexes from cells or tissues.

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2 protocols using pierce agarose chip assay kit

1

ChIP-Seq and RIP Protocols

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For chromatin immunoprecipitation (ChIP) experiments, the Pierce Agarose ChIP Assay Kit (Thermo Fisher Scientific) was used. RNA immunoprecipitation (RIP) was performed using Imprint® RNA Immunoprecipitation Kit (Sigma Aldrich); see Supplementary Methods for protocols.
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2

ChIP-qPCR Assay for SRF Binding

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ChIP assays were performed using the Pierce Agarose ChIP Assay Kit (Thermo Scientific, Catelog # PI26156) according to manufacture instruction. The cross-linked chromatin from subconfluent PAC1 cells in 10% FBS was immunoprecipitated using anti-SRF antibodies (Santa Cruz, sc-335), followed by qPCR using primers specific to the Nik promoter in a region containing the CArG box (Forward primer: 5′—TGTTCAGCCCATTTTTAGGC -3′, Reverse primer: 5′- TTTAGCATTGTGCGAGTGTC -3′) (S2 Table).
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