For neurite outgrowth assays, spheroids were grown in N2B27 media supplemented with 1 mM sodium pyruvate (Gibco), 1 mM sodium L-lactate (Sigma-Aldrich) or 0.5 mM D-Glucose (Sigma-Aldrich) for 48 h on Matrigel-coated plates. For calcium imaging, neuronal cultures were grown in cortical maturation media containing N2B27 with 20 ng/μl BDNF and 20 ng/μl NT-3 and supplemented with 1 mM sodium pyruvate (Gibco) or 1 mM sodium L-lactate (Sigma-Aldrich) for two weeks. Media was changed every alternate day.
Sodium l lactate
Manufactured by Merck Group
Sourced in United States, Germany, Switzerland, China, Spain
Sodium L-lactate is a chemical compound that is used in various laboratory applications. It is the sodium salt of L-lactic acid, a naturally occurring organic acid. Sodium L-lactate is a white, crystalline powder that is highly soluble in water and has a mild, salty taste. It is commonly used as a pH buffer, a source of L-lactate ions, and in the preparation of various cell culture media and other laboratory solutions.
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Lab products found in correlation
Sodium pyruvate, by Merck Group (14 mentions)
D glucose, by Merck Group (13 mentions)
Sodium chloride (nacl), by Merck Group (7 mentions)
L lactic acid, by Merck Group (6 mentions)
Sodium d lactate, by Merck Group (6 mentions)
Rotenone, by Merck Group (5 mentions)
Glucose, by Merck Group (5 mentions)
L glutamine, by Merck Group (4 mentions)
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Glutamine, by Thermo Fisher Scientific (4 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (4 mentions)
Iwr 1, by Merck Group (3 mentions)
Glutaraldehyde, by Merck Group (3 mentions)
Glutamax, by Thermo Fisher Scientific (3 mentions)
Phosphate buffered saline (pbs), by Merck Group (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Sodium bicarbonate, by Merck Group (2 mentions)
Azd3965, by MedChemExpress (2 mentions)
Sodium acetate, by Merck Group (2 mentions)
Gentamicin, by Merck Group (2 mentions)
122 protocols using sodium l lactate
1
Neurite Outgrowth and Calcium Imaging Protocols
2
Synthesis of Strontium L-Lactate Trihydrate
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Example 5
A clear and colorless solution of 9.8 grams (0.08 mole) of sodium L-lactate (Sigma Aldrich) was prepared in 25 mL of deionized water. (Both the sodium L-lactate and the water were analyzed and shown to contain at most trace levels of metal contaminants such as aluminum, arsenic, barium, calcium, cadmium, chromium, lead, mercury, and thallium.) A solution of 11.66 g (0.04 mole) of strontium chloride hexahydrate (Sigma Aldrich) was prepared in 15 mL of deionized water. (The solution cools as the strontium salt dissolves.) (The strontium chloride hexahydrate was analyzed and shown to contain at most trace levels of metal contaminants such as aluminum, arsenic, barium, calcium, cadmium, chromium, lead, mercury, and thallium.) The strontium-containing solution was added to the stirred sodium L-lactate solution and a clear and colorless solution was obtained. Six volumes of acetone were added to the solution, and a white solid formed. The solid was isolated by filtration, washed with an 80:20 (v/v) solution of acetone:water to remove sodium chloride, and dried. The product, strontium L-lactate trihydrate, was obtained in 70% yield. Tests for quality and purity such as strontium analysis, NMR and HPLC analysis of L-lactate, HPLC analysis of organic impurities, determination of sterility and absence of endotoxins, were performed.
3
Modulation of Carbonic Anhydrase IX
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HeLa human cervix cancer adenocarcinoma cells and SiHa human cervix squamous cell carcinoma cells were from ATCC. Cells were routinely cultured in DMEM containing 4.5 g/l glucose, 10% fetal calf serum (Lonza BioWhittaker) and gentamicin (Sandoz) at 37°C in humidified air with 5% CO2. Prior to treatment, cells were kept in sparse culture conditions for one week in order to reduce cell-density-induced CA IX expression. After a week, cells were plated at high density (100,000 cells/cm2) in order to stimulate CA IX expression in normoxia. Attached cells were incubated for 24 h or 48 h in basal DMEM (Sigma Aldrich) supplemented with 1g/L of glucose (Sigma Aldrich), 2 mM UltraGlutamine (Lonza), 1% fetal calf serum, 10 mM NaHCO3 (Sigma Aldrich), gentamicin, phenol red and no pyruvate. Cells were incubated without (control) or with 10 mM sodium L-lactate (Sigma Aldrich) or 10 mM sodium L-lactate together with 100 μM sodium L-ascorbate (Sigma Aldrich).
4
Internalization of HCA1 Receptor
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At 24 hours before the experiment, the medium was replaced by either growth medium containing 10 % FBS or medium without FBS. The following day, the cells were exposed either sodium L-lactate (Sigma-Aldrich, USA) 20 mM in PBS or vehicle, for five, 15, 30 or 60 minutes. To study whether HCA1 was internalized in response to receptor activation, other cells were exposed to sodium L-lactate (20 mM), the HCA1 agonist 3-chloro-5hydroxybenzoic acid (3-Cl-5-OH: Sigma-Aldrich, USA; 40 µM), or PBS (control), in the absence or presence of the -arrestin/AP2-dependent G-protein-coupled (GPCR) endocytosis inhibitor, barbadin 110 . In the cultures treated with barbadin, barbadin was added at 30 minutes prior to the HCA1 agonists. Since 3-Cl-5-OH was dissolved in DMSO, all wells were supplemented with DMSO 1 L per 2mL PBS. The exposure times for the latter experiment were 60 and 120 minutes. The cells were harvested 100 L ice-cold RIPA buffer with protease-and phosphatase inhibitor (Sigma-Aldrich, USA), transferred to Eppendorf tubes and snap frozen in liquid nitrogen before they were stored in -80 °C.
5
Basis Set Simulation for LCModel
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The basis set for LCModel was simulated using the NMRScope-B plugin, which is implemented in jMRUI (Version 6.0 beta) [65 ]. MR spectra for each metabolite were calculated based on a priori knowledge of scalar coupling, chemical shifts, and vendor specific hardware parameters for data acquisition. The metabolites simulated and included in the data evaluation were: sodium-3-hydroxybutyrate (βOHB), acetone natural (Acn), lithium acetoacetate (AcAc), sodium l -lactate (Lac), N-acetyl-l -aspartic acid (NAA), creatine anhydrous (Cr), choline chloride (Cho), l -glutamine (Gln), l -glutamic acid (Glu), and myo-inositol (mI) (all Merck KGaA, Darmstadt, Germany). A line broadening of 3 Hz was applied to each basis spectrum.
Following simulation, the basis set was tested on phantom data, including signals for the respective metabolites at expected in vivo concentrations (Lac 2 mmol/L, NAA 8 mmol/L, Cr 6 mmol/L, Cho 2 mmol/L, Gln 2 mmol/L, Glu 7 mmol/L, mI 4 mmol/L). Concentrations of βOHB, Acn and AcAc were at 1 mM. The frequency, phases and linewidths of the peaks were all constrained relative to the creatine singlet at 3.03 ppm (Figure 3 a).
Following simulation, the basis set was tested on phantom data, including signals for the respective metabolites at expected in vivo concentrations (Lac 2 mmol/L, NAA 8 mmol/L, Cr 6 mmol/L, Cho 2 mmol/L, Gln 2 mmol/L, Glu 7 mmol/L, mI 4 mmol/L). Concentrations of βOHB, Acn and AcAc were at 1 mM. The frequency, phases and linewidths of the peaks were all constrained relative to the creatine singlet at 3.03 ppm (
6
NK-92 Cell Cultivation and Cytotoxicity
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NK-92 and CD276-CAR NK-92 cells were cultivated in NK-92 complete medium with sodium l -lactate (Merck, Darmstadt, Germany) at indicated concentrations for 72 h. pH-values were measured using the LAQUAtwin pH-22 pH-meter (Horiba, Kyoto, Japan) and cells were subsequently used as effector cells in standard calcein release assays as previously described.
7
Standardized Bacterial Culture and Buffer Preparation
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PBS 11 mM, pH 7.4 with
137 mM NaCl and 3 mM KCl was used as the standard buffer for all experiments
unless stated otherwise. Britton–Robinson universal buffer
(BRB) contains 40 mM phosphoric, boric, and acetic acid. Cultivation
of bacteria was routinely done in the Luria-Bertani (LB) medium (10
g/L peptone from casein, 5 g/L yeast extract, and 10 g/L NaCl) with
100 mg/L ampicillin. In the case of cultivating bacteria carrying
the pNIC-CH plasmid for PaLCTO, ampicillin was replaced
with 50 mg/L kanamycin. General medium components were purchased from
Carl Roth; sodium L-lactate, ferrocenium hexafluorophosphate (FcPF6), isopropyl β-D-1-thiogalactopytanoside (IPTG), 2,6-dichlorophenol-indophenol
sodium salt hydrate (DCIP), horseradish peroxidase, sodium glycolate,
1,4-BQ, R-2-hydroxybutyric acid, and S-2-hydroxybutyric acid from
Sigma-Aldrich (Germany); S-2-hydroxyvaleric acid from BLD Pharmatech
Ltd. (Shanghai); 2-hydroxypalmitic acid and 2-hydroxy-n-octanoic acid from TCI (Japan); (S)-2-hydroxybutyric
acid and S-(+)-mandelic acid from Fluorochem Ltd. (United Kingdom);
and 10-acetyl-3,7-dihydroxyphenoxazine (AmplexRed) from Chemodex (Switzerland).
137 mM NaCl and 3 mM KCl was used as the standard buffer for all experiments
unless stated otherwise. Britton–Robinson universal buffer
(BRB) contains 40 mM phosphoric, boric, and acetic acid. Cultivation
of bacteria was routinely done in the Luria-Bertani (LB) medium (10
g/L peptone from casein, 5 g/L yeast extract, and 10 g/L NaCl) with
100 mg/L ampicillin. In the case of cultivating bacteria carrying
the pNIC-CH plasmid for PaLCTO, ampicillin was replaced
with 50 mg/L kanamycin. General medium components were purchased from
Carl Roth; sodium L-lactate, ferrocenium hexafluorophosphate (FcPF6), isopropyl β-D-1-thiogalactopytanoside (IPTG), 2,6-dichlorophenol-indophenol
sodium salt hydrate (DCIP), horseradish peroxidase, sodium glycolate,
1,4-BQ, R-2-hydroxybutyric acid, and S-2-hydroxybutyric acid from
Sigma-Aldrich (Germany); S-2-hydroxyvaleric acid from BLD Pharmatech
Ltd. (Shanghai); 2-hydroxypalmitic acid and 2-hydroxy-n-octanoic acid from TCI (Japan); (S)-2-hydroxybutyric
acid and S-(+)-mandelic acid from Fluorochem Ltd. (United Kingdom);
and 10-acetyl-3,7-dihydroxyphenoxazine (AmplexRed) from Chemodex (Switzerland).
8
Comprehensive Metabolite Profiling Setup
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LC-MS grade acetonitrile was purchased from Fisher. Ammonium acetate, ammonium formate, UDP-GlcNAc, UDP-GalNAc, ammonium hydroxide (NH4OH; 5 m ), G6P, F6P, octanoic acid, docosapentaenoic acid, glucose, glucosamine hydrochloride, oleic acid, sodium l -lactate, sodium pyruvate, l -carnitine, l -glutamine, insulin from bovine pancreas, sodium chloride, sodium bicarbonate, potassium chloride, calcium chloride, magnesium chloride, and potassium phosphate monobasic were obtained from Sigma-Aldrich. [1,2-13C2]UDP-GlcNAc was from Omicron Biochemicals (South Bend, IN), and l -[U-13C5,15N2]glutamine was from CIL (Cambridge Isotope Laboratories, Andover, MA). Water for the LC-MS and GC-MS was purified by a Milli-Q system (Millipore, Montreal). BSA fraction V-fatty acid-free grade was from Gemini Bio-Products (West Sacramento, CA). [U-13C6]glucose (MPE = 99%) was from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). d -[U-13C6]glucosamine hydrochloride (MPE = 99%) was from Omicron Biochemicals. Blebbistatin was from Selleck Chemicals (Houston, TX).
9
Preparation of Weak Acid Solutions
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The weak acid solutions were prepared using the following salts: sodium benzoate (bio extra ≥99.5%, B3420-250G; Sigma-Aldrich, St. Louis, MO); potassium acetate (extrapure; Merck); sodium formate (pro analysis; Merck, Darmstadt, Germany); DL-lactic acid lithium salt (approximately 98%, L1500; Sigma-Aldrich); pyruvic acid-sodium salt (99+%; Acros Organics, Geel, Belgium); succinic acid-disodium salt, anhydrous (99%; Acros Organics); potassium chloride (pro analyses; BOOM Laboratoriumleveranciers, Meppel, The Netherlands); and sodium L-lactate (>99.0%, 71718-10G; Sigma-Aldrich). Lipids were purchased from Avanti Polar Lipids (Alabaster, AL). The following lipids were used: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (DOPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (POPG), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (DPPG).
10
Human iPSC Cardiac Differentiation
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Human iPSC were maintained on hESC-qualified Matrigel(TM) (BD Biosciences) coated plates in mTESR1 (STEMCELL Technologies) medium until they reached 80 to 90 % confluency. Cardiac differentiation was induced by BMP4 (Life technologies) (25 ng/ml) and CHIR99021 (5 μM) (Sigma-Aldrich) in RPMI1640 (Life technologies) medium containing B27 (Life technologies) and 2 mM glutamine (Life technologies) and 50 μg/ml L-Ascorbic acid (Cell culture tested powder; Sigma-Aldrich) as a basal medium. After 24 h cells were kept in the same basal medium with CHIR (5 μM) only for 18–36 h. Afterwards cells were kept in RPMI basal medium with B27 without insulin for 24 h then medium was replaced with similar basal medium having WNT inhibitor either 10 μM of XAV939 (Sigma-Aldrich) or IWR1 (Sigma-Aldrich) for 5 days. Afterwards cells were kept 4 to 5 days in basal medium (B27 + insulin) followed by replacement with cardiac enrichment medium (RPMI 1640 without glucose (Life technologies) + 4 mM sodium L-lactate [32 (link)] (Sigma-Aldrich). Cells were kept in enrichment medium for 4 to 5 days. After enrichment phase medium was switched back to basal medium (RPMI + B27 + glutamine).
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