Photometrics coolsnap fx

Manufactured by Teledyne

Sourced in Germany

The Photometrics CoolSNAP fx is a scientific-grade digital camera designed for low-light imaging applications. It features a high-resolution CCD sensor, advanced cooling capabilities, and a range of connectivity options to enable precise and sensitive data capture.

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Lab products found in correlation

Bx60 microscope, by Olympus (2 mentions) Metaview image analysis system, by Universal Imaging (2 mentions) Metamorph imaging system, by Teledyne (2 mentions) Axio observer 7 microscope, by Zeiss (1 mentions) Orca flash 4 camera, by Hamamatsu Photonics (1 mentions) Metaview, by Universal Imaging (1 mentions) Nanozoomer hamamatsu scanner, by Hamamatsu Photonics (1 mentions)

Close spelling variants of this product

Photometrics Coolsnap FX camera (3 citations)

3 protocols using photometrics coolsnap fx

Multimodal Microscopy Imaging Protocol

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Widefield fluorescence images were taken with an Olympus BX70 microscope (Olympus Optical, Hamburg, Germany) equipped with a CCD Camera (Photometrics CoolSNAP fx; Roper Scientific) and driven by Metaview (Universal Imaging, Downington, PA, USA) and with an Axio Observer 7 microscope (Zeiss) equipped with a motorized stage coupled with an Orca Flash 4 Camera (Hamamatsu), and driven by the Zen software (Zeiss). Confocal images were taken with a Nikon Ti2 microscope equipped with a motorized stage and a Yokogawa CSU-W1 spinning disk head coupled with a Prime 95 sCMOS camera (Photometrics), driven by Metamorph. Serial widefield fluorescence images were acquired using a NanoZoomer Hamamatsu Scanner. Images were then analyzed using Metaview image analysis software (Universal Imaging, Downington, PA, USA) or Imaris Microscopy Image Analysis software. Image J and Fiji were used for quantification analysis.

Roth F., Dhiab J., Boulinguiez A., Mouigni H.R., Lassche S., Negroni E., Muraine L., Marhic A., Oliver A., Lainé J., Rouche A., O’Ferrall E.K., van Engelen B., Ottenheijm C., Greif H., Blumen S., Lacau St Guily J., Perie S., Butler-Browne G., Mouly V, & Trollet C. (2022). Assessment of PABPN1 nuclear inclusions on a large cohort of patients and in a human xenograft model of oculopharyngeal muscular dystrophy. Acta Neuropathologica, 144(6), 1157-1170.

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Microscopic Image Quantification Protocol

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Images were visualized using an Olympus BX60 microscope (Olympus Optical, Hamburg, Germany), digitalised using a CCD camera (Photometrics CoolSNAP fx; Roper Scientific) and analyzed using MetaView image analysis system (Universal Imaging, Downington, PA, USA), MetaMorph imaging system (Roper Scientific, Tucson, AZ, USA) software, and ImageJ 1.44o (http://imagej.nih.gov/ij) for quantification analysis.

Klein P., Oloko M., Roth F., Montel V., Malerba A., Jarmin S., Gidaro T., Popplewell L., Perie S., Lacau St Guily J., de la Grange P., Antoniou M.N., Dickson G., Butler-Browne G., Bastide B., Mouly V, & Trollet C. (2016). Nuclear poly(A)-binding protein aggregates misplace a pre-mRNA outside of SC35 speckle causing its abnormal splicing. Nucleic Acids Research, 44(22), 10929-10945.

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Quantitative Image Analysis Protocol

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Images were visualized using an Olympus BX60 microscope (Olympus Optical, Hamburg, Germany), digitalized using a CCD camera (Photometrics CoolSNAP fx; Roper Scientific) and analyzed using MetaView image analysis system (Universal Imaging, Downington, PA), MetaMorph imaging system (Roper Scientific, Tucson, AZ, USA) software, and ImageJ 1.44o (http://imagej.nih.gov/ij) for quantification analysis.

Malerba A., Roth F., Harish P., Dhiab J., Lu-Nguyen N., Cappellari O., Jarmin S., Mahoudeau A., Ythier V., Lainé J., Negroni E., Abgueguen E., Simonelig M., Guedat P., Mouly V., Butler-Browne G., Voisset C., Dickson G, & Trollet C. (2019). Pharmacological modulation of the ER stress response ameliorates oculopharyngeal muscular dystrophy. Human molecular genetics, 28(10).

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