Pericam psi system

Manufactured by Perimed

Sourced in Sweden, United States, China

The PeriCam PSI System is a non-invasive imaging device used to measure and visualize tissue perfusion. It utilizes laser speckle contrast imaging technology to provide real-time assessment of microvascular blood flow.

Automatically generated - may contain errors

Lab products found in correlation

Pimsoft, by Perimed (13 mentions) Pimsoft 1, by Perimed (4 mentions) Periflux system 5001, by Perimed (2 mentions) Pimsoft software, by Perimed (1 mentions) Stereotaxic frame, by RWD Life Science (1 mentions) Lsm 510 nlo, by Zeiss (1 mentions) Fitc dextran, by Merck Group (1 mentions) Pimsoft program, by Perimed (1 mentions) Avertin, by Merck Group (1 mentions) Pericam psi hr, by Perimed (1 mentions) Flir tools software, by Teledyne (1 mentions) Flir e6, by Teledyne (1 mentions) Model 900, by Kopf Instruments (1 mentions)

98 protocols using pericam psi system

Scarring Assessment and Wound Healing Evaluation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Patient and Observer Scar Assessment Scale (POSAS) included the Patient Scar Assessment Scale (PSAS) and the Observer Scar Assessment Scale (OSAS), which were used to evaluate the surgical improvement at 3-, 6- and 12-month follow-up. The PSAS includes six items: pain, pruritus, color, stiffness, thickness and irregularity, while the 6 items included in OSAS are: vascularity, pigmentation, thickness, relief, pliability, and surface. Each item was graded on a 10-point scale (1 represented the best and 10 represented the worst), and the scores on all items were summed to obtain a total score ranging from 6 to 60 [26 (link)]. Recurrence of PS was defined as a POSAS thickness score 5. The aspect and scar width were individually monitored for 18 months [27 (link)]. Topical perfusion was measured with the PeriCam PSI System (Perimed, Sweden) using the Region of Interests (ROIs) method.
Wound eversion was objectively assessed with a ruler on the adjacent flat skin surface parallelly aside from the midpoint. Data were collected immediately after surgery and at 3, 6 and 12 months postoperatively. Complications such as wound dehiscence, seroma or hematoma, infection and spitting sutures were observed and recorded for at least 12 months after surgery.

Min P., Zhang S., Sinaki D.G., Yao P., Hu F., Wang X., Zhou D., Chai J, & Zhang Y. (2023). Using Zhang’s supertension-relieving suture technique with slowly-absorbable barbed sutures in the management of pathological scars: a multicenter retrospective study. Burns & Trauma, 11, tkad026.

+ Open protocol

+ Expand

Cerebral Blood Perfusion Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

CBP was assessed using a blood perfusion imager (PeriCamPSI System, Perimed AB, Stockholm, Sweden) based on a laserspeckle contrast imaging (LSCI) technology. Briefly, the skull of the mouse was exposed by midline skin incision, and the cerebral blood flow was detected through the skull. The unit of CBP is expressed in PU (perfusion units, PU).

Chen J.F., Liu K., Hu B., Li R.R., Xin W., Chen H., Wang F., Chen L., Li R.X., Ren S.Y., Xiao L., Chan J.R, & Mei F. (2021). Enhancing myelin renewal reverses cognitive dysfunction in a murine model of Alzheimer’s disease. Neuron, 109(14), 2292-2307.e5.

+ Open protocol

+ Expand

Laser Speckle Contrast Imaging for Skin Perfusion

Check if the same lab product or an alternative is used in the 5 most similar protocols

A Laser Speckle Contrast Imager (PeriCam PSI System, Perimed AB, Järfälla, Sweden) was used to measure the perfusion of the skin. The measurement principle of LSCI has previously been described in detail^{13 (link)}. This system uses a divergent class 1 laser with a wavelength of 785 nm that illuminates the skin. The laser light creates a fluctuating speckle pattern due to optical interference. Due to the finite exposure time of the camera, these fluctuations result in a decrease in the contrast between the speckles. The local speckle contrast in the image is therefore correlated to the perfusion in the skin. From this pattern, a perfusion value is calculated, given as perfusion units (PU), an arbitrary unit proportional to the concentration and mean velocity of red blood cells. The same acquisition parameters were used for all perfusion measurements. The measurement distance was between 20 and 30 cm. The sampling rate was set to 21 images per second, 5 images were used to construct an average image giving an effective frame rate of 4.2 images per second. The system was calibrated at regular intervals as recommended by the manufacturer.

Elawa S., Mirdell R., Stefanis A., Tesselaar E, & Farnebo S. (2024). Microcirculatory changes in the skin after postmastectomy radiotherapy in women with breast cancer. Scientific Reports, 14, 4149.

+ Open protocol

+ Expand

Middle Cerebral Artery Occlusion in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Focal ischemia was induced by intraluminal middle cerebral artery occlusion (MCAO) for 5 min, 3 h and 24 h using a monofilament nylon string coated with silicon (MSMC23B104PK100, RWD Life Science, Shenzhan, China) in male Balb/c mice under isoflurane anesthesia as described earlier (Wang et al., 2004 (link); Krawiec et al., 2009 (link)). Physiological parameters (PaO₂, PaCO₂, blood pressure) were monitored, and rectal temperature was controlled at 37.0 ± 0.5°C. Cortical cerebral blood flow was monitored using a blood perfusion imager (PeriCam PSI System, Perimed AB, Stockholm, Sweden). None of the animals showed any adverse effects before euthanasia. Animal blood was extracted after different time points of surgery. Sham-operated mice underwent the same surgical procedure except that MCAO was used for the 0 min time point.

Lu D., Ho E.S., Mai H., Zang J., Liu Y., Li Y., Yang B., Ding Y., Tsang C.K, & Xu A. (2020). Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke. Frontiers in Neuroscience, 14, 81.

+ Open protocol

+ Expand

Cerebral Blood Flow Changes After CCI

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess the cerebral blood flow (CBF) changes in the cortex at pre-surgery and at 1 h, 12 h, 1 day, and 3 days after CCI, we used a laser speckle contrast imaging (LSCI) system (PeriCam PSI System, Perimed AB, Sweden) as we previously described [23 (link)]. Briefly, the mice were anesthetized and placed prone in a stereotaxic head frame. Then, a midline incision was made over the skull to expose the calvaria. The exposure area was kept clean and dry using a tampon during image collection. The CBF was continuously measured for 60 s at the following settings: observation height, 10 cm; laser irradiation area, 2 × 2 cm; image matrix, 1388 × 1038. We select the whole cerebral cortex (100 mm²) as the region of interest (ROI). The CBF changes were evaluated using the vendor-supplied PIMsoft software (version 1.4; Perimed) [23 (link)]. The measurement and analysis of laser speckle were done by investigators blinded to experimental groups.

Shi G., Liu L., Cao Y., Ma G., Zhu Y., Xu J., Zhang X., Li T., Mi L., Jia H., Zhang Y., Liu X., Zhou Y., Li S., Yang G., Liu X., Chen F., Wang B., Deng Q., Zhang S, & Zhang J. (2023). Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injury. Journal of Neuroinflammation, 20, 222.

+ Open protocol

+ Expand

Photochemical Thrombosis for Focal Ischemic Stroke

Check if the same lab product or an alternative is used in the 5 most similar protocols

Photochemical thrombosis method was used for inducing the focal brain cortical ischemic stroke in adult male mice [28] , [29] . After anesthesia via inhalation of isoflurane, stereotaxic device was used for keeping the mice stable for coordination. Mouse skull was then exposed and a diode-pumped solid-state laser light with a wavelength of 532 nm, power of 0.5 mW, and diameter of 2-mm (GL532TA-100FC, Laser & Optics Century, Shanghai) was positioned at the left side 2.2 mm from fontanelle of the brain and 0.5 mm anteriorly. Rose Bengal (10 mg/mL) was administered intraperitoneally to 0.3 mL/30 g. After 5 min, the laser power of 80 mW was used for irradiation at the brain for 15 min. A Laser Speckle Contrast Imaging System (PeriCam PSI System, Perimed AB, Stockholm, Sweden) was used to monitor the blood flow in the brain to confirm the success of blood flow occlusion by the PT surgery. Rectal temperature of the mice was maintained at 37 °C during the surgery. Moues head skin was sutured after surgery and they were then transferred back to their cages for recovery.

Tsang C.K., Mi Q., Su G., Hwa Lee G., Xie X., D'Arcangelo G., Huang L, & Steven Zheng X.F. (2022). Maf1 is an intrinsic suppressor against spontaneous neural repair and functional recovery after ischemic stroke. Journal of Advanced Research, 51, 73-90.

+ Open protocol

+ Expand

Quantifying Cutaneous Blood Perfusion by LSCI

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cutaneous blood perfusion quantification was performed with laser speckle contrast imaging (LSCI; PeriCam PSI System, Perimed AB, Järfälla, Sweden), as previously described.³⁰ In short, assessments were performed in a temperature‐controlled room (22°C) after acclimatization of the subjects. LSCI recordings of the target area on the left and right ventral forearms were captured with the use of dedicated software (PimSoft, Perimed AB). Circular regions of interest at the intradermal injection sites were defined and cutaneous blood perfusion (indicated as basal flow) was quantitatively assessed and expressed in arbitrary units (AUs). The homogeneity of cutaneous blood perfusion in the region of interest (indicated as flare), expressed as values that are +1 standard deviation (SD) from the mean basal flow within the region, was also quantitatively assessed and expressed in AUs. Illustrations of cutaneous blood perfusion measured with LSCI are depicted in Figure 1.

Saghari M., Gal P., Gilbert S., Yateman M., Porter‐Brown B., Brennan N., Quaratino S., Wilson R., Grievink H.W., Klaassen E.S., Bergmann K.R., Burggraaf J., van Doorn M.B., Powell J., Moerland M, & Rissmann R. (2022). OX40L Inhibition Suppresses KLH‐driven Immune Responses in Healthy Volunteers: A Randomized Controlled Trial Demonstrating Proof‐of‐Pharmacology for KY1005. Clinical Pharmacology and Therapeutics, 111(5), 1121-1132.

+ Open protocol

+ Expand

Cutaneous Microvascular Flow Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

A laser speckle contrast imaging system with a laser wavelength of 785 nm (PeriCam PSI system, Perimed, Järfälla, Sweden) coupled to iontophoresis of acetylcholine and sodium nitroprusside will measure non-invasively real-time cutaneous microvascular flow changes in the forearm [42 (link)]. For the post-occlusive reactive hyperemia (PORH) test, arterial occlusion will be performed with suprasystolic pressure (50 mmHg above the systolic arterial pressure) using a sphygmomanometer applied to the arm of the subject over 3 min. Peak skin flow will be measured after pressure release.
Images will be analyzed using the manufacturer’s software (PIMSoft, Perimed, Järfälla, Sweden). The measurements of skin blood flow will be divided by the mean arterial pressure to yield the cutaneous vascular conductance (CVC) in arbitrary perfusion units (APU)/mmHg, to avoid interference of blood pressure levels on calculation of microvascular flow.

dos Santos E.M., de Moraes R., Tibiriça E.V., Huguenin G.V., Moreira A.S, & De Lorenzo A.R. (2018). Whey protein supplementation for the preservation of mass and muscular strength of patients with heart failure: study protocol for a randomized controlled trial. Trials, 19, 431.

+ Open protocol

+ Expand

Noninvasive Cerebral Blood Flow Monitoring

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cerebral cortical blood flow was monitored for 24 h post-TBI using a non-invasive laser speckle imager with a 70 mW built-in laser and a 1388 × 1038-pixel CCD camera (PeriCam PSI System, Perimed, Sweden) as recently described [23 (link)]. Briefly, mice were sedated with isoflurane and placed on a temperature-controlled stereotaxic frame (RWD Life Science, San Diego, CA, USA), with the skull exposed. The CCD camera was installed 10 cm above the skull to capture the CBF images in a scanning area of 2.0 × 2.0 cm. The ipsilateral cortical blood flow (perfusion unit, PU) was calculated and presented as mean perfusion values using the vendor-supplied PIMSoft software (version 1.5; Perimed, Jakobsberg, Sweden).

Li T., Li L., Peng R., Hao H., Zhang H., Gao Y., Wang C., Li F., Liu X., Chen F., Zhang S, & Zhang J. (2022). Abrocitinib Attenuates Microglia-Mediated Neuroinflammation after Traumatic Brain Injury via Inhibiting the JAK1/STAT1/NF-κB Pathway. Cells, 11(22), 3588.

+ Open protocol

+ Expand

Scar Evaluation by Blood Perfusion

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The physicians also recorded the frequency of infection. A standard frontally oriented photograph was taken at 3, 6-month, and 1 year follow-up clinic.
The features of each scar were objectively evaluated measuring the changes of blood supply. The PeriCam PSI System® (Perimed, Järfälla, Sweden) blood perfusion imager was applied to assess changes of the blood supply within the scar. The PeriCam PSI System® is a blood perfusion imager 70-mW system based on Laser Speckle Contrast Analysis (LASCA) technology utilizing a laser wavelength of 785 nm [20 (link)].

Li K., Nicoli F., Xi W.J., Zhang Z., Cui C., Al-Mousawi A., Balzani A., Tong Y, & Zhang Y. (2019). The 1470 nm diode laser with an intralesional fiber device: a proposed solution for the treatment of inflamed and infected keloids. Burns & Trauma, 7, 5.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!