Female 3×Tg-AD mice (APPswe, PSEN1M146V, and MAPTP301L transgenes) were provided by the Jackson Laboratory. The age-matched wild-type mice (female B6129SF2/J mice) (WT mice) were purchased from Guangdong Medical Laboratory Animal Center (Guangdong, China). The local reproduce was kept at the Shenzhen Center for Disease Control and Prevention (Shenzhen, China). The mice were kept with a 12 h artificial light/dark cycle (dark on 19:00–07:00) and able to get food and tap water freely. Eight-month old female 3×Tg-AD mice (12 mice at least in each group) were treated with TBN (60 mg/kg, i. g., bid), donepezil (1.3 mg/kg, i. g., qd), memantine (5 mg/kg, i. g., bid) or saline by gastric gavage for 4 months. The time gap between two administration was 8 h (9 a.m. and 5 p.m.). Eight-month old female WT mice were received with parallel volume of saline as a control group. After behavioral tests, mice were sacrificed with injection anesthetics (Zoletil 50/xylazine, ip), and brain tissues were collected for biochemistry analysis or proteomics analysis. All procedures were approved and supervised by the Institutional Animal Care and Use Committee of Shenzhen Center for Disease Control and Prevention.
Mapt p301l
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro
MAPT P301L is a recombinant protein product manufactured by Jackson ImmunoResearch. It is a mutant form of the microtubule-associated protein tau (MAPT), specifically the P301L mutation. This product is intended for use in research applications.
Automatically generated - may contain errors
5 protocols using mapt p301l
1
Alzheimer's Disease Mouse Model Study
2
Fasudil Treatment in Triple Transgenic Alzheimer's Mice
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Triple Transgenic mice carrying Swedish FAD APP695, PSEN M146V and MAPT P301L were obtained from The Jackson Laboratory, bred and aged to 18 months, N = 17 (9 female, 8 male). Fasudil HCL (10 mg/kg) or an equivalent vol of vehicle (0.9% saline) was administered via intraperitoneal injection once a day for 14 consecutive days. On the final day, the animals received a further injection (number 15) and then were sacrificed 1 h later by cervical dislocation, brains rapidly removed, hemisected, half being flash frozen on liquid 2-methylbutane at −55 °C for biochemical analysis and the remaining half post-fixed in 4% PFA for immunohistochemical staining. Tissue samples. Rostral pole of frozen frontal lobe were cut weighed and homogenised on ice in a 8 x volume of PBS to which protease inhibitors (Roche), phosphatase inhibitors (Phos-STOP), NaF (10 mM), and β-glycerol phosphate (2 mM) had been added. Of the N = 17 mice 10 (4 male, 6 female) received vehicle and 7 (4 male, 3 female) fasudil. The homogenates were subjected to centrifugation at 100,000 g at 4 °C for 20 min. The supernatant (S1) and pellet (P1) were collected and stored with at −80 °C.
3
Transgenic Mouse Model of Alzheimer's
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3 × Tg mice (human APP KM670/671NL (Swedish), MAPT P301L, and PSEN1 M146 V) exhibiting amyloid and tau pathologies and B6129S control mice were purchased from the Jackson Laboratory (Bar Harbor, ME) and were used to conduct the experiments described. The animals were maintained in an environmentally controlled room at 22° ± 1°C with a 12-h light/dark cycle in a specific pathogen-free facility at the East China Normal University (Shanghai, China). All mice were housed in clear polycarbonate micro-isolator cages (five mice per cage), allowed free access to water and food. Both males and females were included in approximately equal ratios for all experiments. The detailed number, age, and sex of mice used for each experiment are shown in the figure legends. All procedures in the animal experiments were approved by the Institutional Animal Care and Use Committee of East China Normal University. All methods were performed in accordance with the relevant guidelines and regulations.
4
Alzheimer's Disease Progression in 3xTg-AD Mice
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Homozygous 3xTg-AD mice carrying three mutations associated with familial Alzheimer’s disease (APP KM670/671NL Swedish, PSEN1 M146V, MAPT P301L) [72 (link)] were obtained from Jackson Laboratories (Bar Harbor, ME). The mice are viable, fertile, and display no initial gross physical or behavioral abnormalities, and develop age-related progressive neuropathology including amyloid plaques (which appear by 6 months) and Tau tangles (by 12 to 15 months). Behavioral tests were performed on 8-month-old 3,Tg-AD or non-transgenic C57BL/6 male mice. All mice were bred and maintained in a vivarium at 22 °C in a 12 h light/dark cycle, with food and water available ad libitum. Mice were housed according to the Federation of Laboratory Animal Science Associations (FELASA) guidelines, and all the experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) at Bar-Ilan University, Faculty of Medicine (protocol number 60-09-2019).
5
Transgenic Mouse Models of Alzheimer's Disease
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In this study two animal models of AD were used. Seven and 12 mo 3xTg male mice, harboring three mutations associated with familial AD (APPSwe, MAPTP301L, and PS1M146V) on a congenic C57BL/6J background, and control littermates were purchased by The Jackson Laboratory (Stock number 008880) (10 (link), 24 (link)). 5XFAD mice, which co-overexpress a triple-mutant human APP (Swedish mutation: K670N, M671L; Florida mutation: I716V; London mutation: V717I) and a double-mutant human PS1 (M146L and L286V mutations), and B6SJL mice were purchased from Jackson Laboratories and bred at Centro Stabulario Interdipartimentale, Unimore. Experimental 3 and 5 mo 5XFAD mice were obtained by crossing hemizygous 5XFAD mice with B6SJL breeders. All mice were kept in conditioned rooms with stable temperature (21°C) and humidity (60%), on a light/dark cycle of 12 hours (h). Food and water were available ad libitum and body weight was recorded throughout the entire observation period. All animal procedures were approved by the Committee on Animal Health and Care of the University of Modena and Reggio Emilia and Italian Ministry of Health (protocol number: n°974/2016-PR) and conducted in accordance with National Institutes of Health guidelines.
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