The GC-MS/MS method was performed on a 7890A gas chromatography (Agilent) coupled to a triple quadrupole 7000A (Agilent) equipped with a High sensitivity electronic impact source (EI) operating in positive mode. The front inlet temperature was 250°C, the injection was performed in splitless mode. The transfer line and the ion-source temperature were 250°C and 230°C, respectively. The septum purge flow was fixed at 3 mL/min, the purge flow to split vent operated at 80 mL/min during 1 min and gas saver mode was set to 15 mL/min after 5 min. The helium gas flowed through the column (J&WScientificHP-5MS, 30m x 0.25 mm, i.d. 0.25 um, d.f., Agilent Technologies Inc.) at 1 mL/min. Column temperature was held at 60°C for 1 min, then raised to 210°C (10°C/min), followed by a step to 230°C (5°C/min) and reached 325°C (15°C/min), and be hold at this temperature for 5 min. The collision gas was nitrogen. The scan mode used was the MRM for biological samples. Peak detection and integration of the analytes were performed using the Agilent Mass Hunter quantitative software (B.07.01).
J w scientific hp5 ms
Manufactured by Agilent Technologies
Sourced in Spain
The J&W Scientific HP5-MS is a gas chromatography capillary column designed for general-purpose applications. It features a 5% phenyl-methylpolysiloxane stationary phase that provides good separation for a wide range of analytes. The column has a length of 30 meters, an internal diameter of 0.25 millimeters, and a film thickness of 0.25 micrometers.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using j w scientific hp5 ms
1
GC-MS/MS Analysis of Biological Samples
2
Quantitative Determination of α-Ketoglutarate in Serum by GC/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aplha-ketoglutarate was quantitatively determined in serum samples by GC/MS at the Centre for Omic Sciences (Rovira i Virgili University). The methodology used has been reported elsewhere [10 (link)]. Samples were analysed in a 7890A Series gas chromatograph coupled to a 7200 GCqTOF MS (Agilent Technologies, Santa Clara, U.S.A.). The chromatographic column was a J&W Scientific HP5-MS (30 m x 0.25 mm i.d., 0.25 μm film) (Agilent Technologies). The calibration curve showed good linearity in the studied range of 0.13 to 10 mg/L, with a determination coefficient (R2) of 0.9993. The extraction recovery was 99.7%, while accuracy was 101.7%. The intraday and interday precision had a relative standard deviation of, respectively, 4.1% and 4.8% (RSD, n = 3). MDL was 0.05 mg/L, while MQL was 0.13 mg/L.
3
GC-QqQ Analysis of Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following the procedure described in Hernandez-Alonso et al. [34 (link)], samples were analyzed in a 7890A Series GC coupled to a triple quadrupole (QqQ) (7000 series; Agilent Technologies, Barcelona, Spain) using the J&W Scientific HP5-MS (30 m × 0.25 mm i.d., 0.25 µm film; Agilent Technologies, Barcelona, Spain) chromatographic column and helium as a carrier gas. Ionization was carried out with electronic impact recording data in “Full Scan” mode.
Metabolite measurements were based on specific RT plus an ion fragmentation pattern. Quantification was performed by internal standard calibration, using the corresponding analytical standard for each determined metabolite (succinic d4 acid, glycerol 13C3, norvaline, L-methionine-(carboxy-13C, methyl-d3), D-glucose 13C6, myristic-d27 acid, and alpha-tocopherol d6), and a deuterated internal standard depending on the family of metabolite.
Metabolite measurements were based on specific RT plus an ion fragmentation pattern. Quantification was performed by internal standard calibration, using the corresponding analytical standard for each determined metabolite (succinic d4 acid, glycerol 13C3, norvaline, L-methionine-(carboxy-13C, methyl-d3), D-glucose 13C6, myristic-d27 acid, and alpha-tocopherol d6), and a deuterated internal standard depending on the family of metabolite.
4
Quantitative Analysis of Bacterial Endotoxin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The LPS concentration was determined by quantifying the amount of 3OHFAs by gas chromatography coupled to triple quadrupole mass spectrometer (GC-QqQ/MS). 3OHFAs are anchored to a phosphorylated glucosamine disaccharide and are chemical markers of endotoxin [22 (link)]. In this work, 3OHFAs with chain lengths of 8–18 carbons were detected in samples using a method based on the alkaline hydrolysis of LPS, trimethylsilyl derivatization of the obtained 3OHFAs and analysis by GC-QqQ/MS. Analysis was performed on a 7890A Series gas chromatograph coupled to a 7000 GCQqQ (Agilent Technologies, Santa Clara, CA, USA). Chromatographic column was a J&W Scientific HP5-MS (30 m × 0.25 mm i.d., 0.25 μm film) (Agilent Technologies).
Since 3OHFA products from mitochondrial fatty acid β-oxidation can represent a limitation that interferes with the analysis [23 (link)], total and free 3OHFAs were analysed, and the 3OHFA concentration from LPS was obtained as the difference between the two concentrations. Finally, the total LPS content was calculated as the sum of the number of nanomoles of the individual 3OHFAs divided by 4 to account for the four 3OHFA molecules assumed to be present per molecule of LPS [24 (link)].
Since 3OHFA products from mitochondrial fatty acid β-oxidation can represent a limitation that interferes with the analysis [23 (link)], total and free 3OHFAs were analysed, and the 3OHFA concentration from LPS was obtained as the difference between the two concentrations. Finally, the total LPS content was calculated as the sum of the number of nanomoles of the individual 3OHFAs divided by 4 to account for the four 3OHFA molecules assumed to be present per molecule of LPS [24 (link)].
5
GC-QqQ Analysis of Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples were analysed in a 7890 A Series GC coupled to a triple quadrupole (QqQ) (7000 series; Agilent Technologies, Barcelona, Spain). The chromatographic column was a J&W Scientific HP5-MS (30 m × 0.25 mm i.d., 0.25 µm film; Agilent Technologies, Barcelona, Spain), and helium (99.999% purity) was used as a carrier gas. Ionization was carried out with electronic impact recording data in “Full Scan” mode.
Quantification was performed by internal standard calibration, using the corresponding analytical standard for each determined metabolite and a deuterated internal standard depending on the family of metabolite. The internal standards used were succinic d4 acid, glycerol 13C3, norvaline, L-methionine-(carboxy-13C,methyl-d3), D-glucose 13C6, myristic-d27 acid and alpha-tocopherol d6.
Quantification was performed by internal standard calibration, using the corresponding analytical standard for each determined metabolite and a deuterated internal standard depending on the family of metabolite. The internal standards used were succinic d4 acid, glycerol 13C3, norvaline, L-methionine-(carboxy-13C,methyl-d3), D-glucose 13C6, myristic-d27 acid and alpha-tocopherol d6.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!