B6 cg gt rosa 26sortm3 cag eyfp hze j

All animals used in this study received humane care in compliance with the principles stated in the Guide for the Care and Use of Laboratory Animals, NIH Publication, 1996 edition, and the protocol was approved by the Institutional Animal Care Committee of Universityof Washington. The C57BL/6 and SCID/Beige mice were purchased from Charles River (Wilmington, MA). Lgr5-DTR-eGFP mice were originally generated by Genentech (Tian et al., 2011 (link)) and were kindly provided by Dr. Noah Shroyer at the Baylor College of Medicine. Krt7-CreER^T2 was kindly provided by Dr. Jianwen Que at Columbia University. Krt8-CreER^T2 was made by our laboratory as described (Zhang et al., 2012 ). C57BL/6-Gt(ROSA)26Sor^{tm1(HBEGF)Awai}/J, B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J, B6.129P2-Lgr5^{tm1(cre/ERT2)Cle}/J, Col1a2-CreER^T2, Pdgfrβ-CreER^T2, NG2-CreER^T2, B6.Cg-Gt(ROSA)26Sor^{tm3(CAG-EYFP)Hze}/J mice were from the Jackson Laboratory (Bar Harbor, ME). Male mice at the age of E15.5 to postnatal 27 weeks were used. Mice were genotyped by polymerase chain reaction using mouse genomic DNA from tail biopsy specimens. The sequences of genotyping primers and the expected band sizes for PCR are listed in Table S3. PCR products were separated electrophoretically on 1% agarose gels and visualized via ethidium bromide under UV light.

All animals were cared for under the provisions of the University of Virginia Animal Care and Use Committee. Mice, 10–25 weeks old, were fed normal mouse chow (0.3% sodium; (Envigo, Cat#7012). Renin Cre recombinase (Ren1^d-Cre) mice^{43 (link)}, were mated with mice harboring lox-P recombination sites flanking exon 3 of Pannexin 1 (Panx1^fl/fl) or wildtype alleles (Panx1^wt/wt)^{79 (link)} to produce conditional Pannexin 1 knockout mice (Ren1-Panx1^Δ/Δ) and Cre-containing control animals (Ren1-Panx1^wt/wt). Panx1 animals were mated with R26R-EYFP transgene (B6.Cg-Gt(ROSA)26Sor^{tm3(CAG-EYFP)Hze}/J) mice purchased from Jackson Laboratory (Stock No: 007903) to create Ren1-Panx1^{Δ/Δ (EYFP)} mice.

All animals used in this study received humane care in compliance with the principles stated in the Guide for the Care and Use of Laboratory Animals, NIH Publication, 1996 edition, and the protocol was approved by the Institutional Animal Care Committee of Baylor College of Medicine and University of Washington. The C57BL/6 and SCID/Beige mice were purchased from Charles River (Wilmington, MA). Col1a2-CreER^T2, and B6.Cg-Gt(ROSA)26Sor^{tm3(CAG-EYFP)Hze}/J mice were purchased from the Jackson Laboratory (Bar Harbor, ME). TCF/LEF:H₂B-GFP reporter line, Ctnnb1^loxp/loxp, and Ctnnb1^lox(ex3) were described previously (Ferrer-Vaquer et al., 2010 (link); Harada et al., 1999 (link); Huelsken et al., 2001 (link)) and were obtained from Dr. Hoang Nugyen at the Baylor College of Medicine. Mice were genotyped by polymerase chain reaction using mouse genomic DNA from tail biopsy specimens. The sequences of genotyping primers and the expected band sizes for PCR are listed in Supplementary Table 2. PCR products were separated electophoretically on 1% agarose gels and visualized via ethidium bromide under UV light.