Log In Sign up
The largest database of trusted experimental protocols

Innotest sandwich elisa

Manufactured by Fujirebio
Visit Supplier
Sourced in Belgium

The INNOTEST sandwich ELISA is a laboratory equipment product designed for the detection and quantification of specific analytes in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to provide accurate and sensitive measurements. The core function of this product is to facilitate the analysis of target molecules through the specific binding of antibodies and the generation of a measurable signal.

Automatically generated - may contain errors

Lab products found in correlation

Polypropylene tube, by Sarstedt (2 mentions) Lobind microtubes, by Eppendorf (1 mentions)

7 protocols using innotest sandwich elisa

1

CSF Biomarkers for Alzheimer's Diagnosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
CSF was acquired following standard procedures. The CSF was obtained by LP between the L3/L4 or L4/L5 intervertebral space, using a 22-gauge atraumatic needle. Five hundred microlitres of CSF were used to perform standardised commercially available INNOTEST sandwich ELISA according to the manufacturer’s instructions (Fujirebio Europe NV, formerly Innogenetics NV). A CSF profile of the Alzheimer’s type was defined by a phosphorylated tau/Aß42 ratio >0.11.6 26 (link) CSF biomarkers results were available in the ECR database but not in the BNA.
Epelbaum S., Paquet C., Hugon J., Dumurgier J., Wallon D., Hannequin D., Jonveaux T., Besozzi A., Pouponneau S., Hommet C., Blanc F., Berly L., Julian A., Paccalin M., Pasquier F., Bellet J., Boutoleau-Bretonniere C., Charriau T., Rouaud O., Madec O., Mouton A., David R., Bekadar S., Fabre R., Liegey E., Deberdt W., Robert P, & Dubois B. (2019). How many patients are eligible for disease-modifying treatment in Alzheimer’s disease? A French national observational study over 5 years. BMJ Open, 9(6), e029663.
+ Open protocol
+ Expand
2

Standardized CSF Collection and Analysis Protocols

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
CSF from individuals with AD and AMC in the WashU‐A cohort were collected via a catheter as previously described.21 (link) CSF from AMC and individuals with symptomatic AD, PSP, CBS, and bvFTD in the WashU‐B cohort were obtained via LP with gravity collection and centrifugation as previously described.22 (link) CSF from MAPT mutation families was collected according to the standardized protocol at the Biomarker Core at the Washington University School of Medicine.32 CSF from individuals with brain tumors was obtained via lumbar drain using a catheter before or after surgery.
CSF from the Montpellier cohort was collected using the standardized protocol for the collection, centrifugation, and storage at Memory Resources and Research Center of Montpellier.33 (link), 34 (link), 35 (link) Briefly, the atraumatic needle was used for LP, with CSF collected into 10 mL polypropylene tube (ref 62.610.201, Sarstedt, Germany) and protein low binding Eppendorf® tubes (LoBind microtubes Eppendorf, ref 022431064, Hamburg, Germany). CSF was not centrifuged before aliquoting and storage at −80°C. CSF tau and pT181 concentrations were measured using the standardized commercially available INNOTEST sandwich ELISA X‐MAP following Fujirebio instructions. CSF Aβ 42 and Aβ 40 were measured using INNOTEST sandwich ELISA from Fujirebio.
Sato C., Mallipeddi N., Ghoshal N., Wright B.A., Day G.S., Davis A.A., Kim A.H., Zipfel G.J., Bateman R.J., Gabelle A, & Barthélemy N.R. (2021). MAPT R406W increases tau T217 phosphorylation in absence of amyloid pathology. Annals of Clinical and Translational Neurology, 8(9), 1817-1830.
+ Open protocol
+ Expand
3

CSF Biomarker Measurement for Alzheimer's

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
CSF biomarkers were measured using standardized commercial Innotest sandwich ELISA (Fujirebio, n = 152) or Euroimmun ELISA method (n = 2) according to the manufacturer’s instructions. Sandwich ELISA relies on two antibodies for detection, one targeting the first 6 N-terminal amino acids (3D6) and the second the 6 C-terminal amino acids (21F12). The Aβ42/40 cutoff used to distinguish Aβ+ from Aβ− individuals was 0.05, as preconized for clinical setting [23 (link)]. For the AT(N) research framework, participants were considered “A+” if CSF Aβ42 was < 500/700 pg/ml depending on the nature of the polypropylene collection tubes or if CSF Aβ42/40 ratio < 0.05/0.1 according to ELISA technic [24 (link), 25 (link)]; “T+” if CSF p-tau181 was > 60 pg/ml; and N+ if CSF t-tau was > 400 pg/ml.
Hirtz C., Busto G.U., Bennys K., Kindermans J., Navucet S., Tiers L., Lista S., Vialaret J., Gutierrez L.A., Dauvilliers Y., Berr C., Lehmann S, & Gabelle A. (2023). Comparison of ultrasensitive and mass spectrometry quantification of blood-based amyloid biomarkers for Alzheimer’s disease diagnosis in a memory clinic cohort. Alzheimer's Research & Therapy, 15, 34.
+ Open protocol
+ Expand
4

Retrospective Study of Amnestic AD and bvFTLD

Check if the same lab product or an alternative is used in the 5 most similar protocols
We performed a retrospective study (2007–2014) collecting patients with an initial clinical suspicion of amnestic AD or bvFTLD with onset after 65 years, from the CSF database of the Gui de Chauliac University Hospital (N = 518). All the patients signed a written informed consent approved by the local ethics committee (registered DC-2008-417). We considered only patients with available clinical and CSF data (n = 152). To limit possible confounding factors and alternative diagnosis, patients with psychiatric conditions able to explain the cognitive and behavioral alterations or with severe vascular burden (Fazekas score = 3) [10] (link), [11] (link) were excluded, as well as patients with prominent aphasic or extrapyramidal presentations; 44 patients were finally retained.
CSF was collected in polypropylene tubes with standardized conditions [12] . CSF Aβ1–42, T-tau, and P-tau were simultaneously measured in every sample using standardized commercially available Innotest sandwich ELISA according to manufacturer's procedures (Fujirebio Ghent Belgium).
Marelli C., Gutierrez L.A., Menjot de Champfleur N., Charroud C., De Verbizier D., Touchon J., Douillet P., Berr C., Lehmann S, & Gabelle A. (2015). Late-onset behavioral variant of frontotemporal lobar degeneration versus Alzheimer's disease: Interest of cerebrospinal fluid biomarker ratios. Alzheimer's & Dementia : Diagnosis, Assessment & Disease Monitoring, 1(3), 371-379.
+ Open protocol
+ Expand
5

Cerebrospinal Fluid Biomarker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lumbar puncture was offered to all participants but was not mandatory, and CSF centralized measurements of β-amyloid 42 peptide (Aβ42), Aβ40, total tau, and phosphorylated tau levels were performed using the standardized INNOTEST sandwich ELISA (Fujirebio).
Frison E., Proust-Lima C., Mangin J.F., Habert M.O., Bombois S., Ousset P.J., Pasquier F., Hanon O., Paquet C., Gabelle A., Ceccaldi M., Annweiler C., Krolak-Salmon P., Béjot Y., Belin C., Wallon D., Sauvee M., Beaufils E., Bourdel-Marchasson I., Jalenques I., Chupin M., Chêne G, & Dufouil C. (2021). Diabetes Mellitus and Cognition: Pathway Analysis in the MEMENTO Cohort. Neurology, 97(8), e836-e848.
+ Open protocol
+ Expand
6

CSF Amyloid-β1-42 Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
CSF was collected at baseline by lumbar puncture using a 25-gauge needle in polypropylene tubes (Sarstedt, Nümbrecht, Germany). CSF was centrifuged at 1800 × g for 10 min at 4 °C and stored at – 20 °C until biomarker analysis, within 2 months after collection. CSF Aβ1–42 was measured using InnoTest sandwich ELISAs (Innogenetics, Fujirebio, Ghent, Belgium) [25 (link)]. Subjects were classified as amyloid positive or negative with a cut-off point of CSF Aβ1–42 < 640 ng/L [26 (link)].
ten Kate M., Barkhof F., Visser P.J., Teunissen C.E., Scheltens P., van der Flier W.M, & Tijms B.M. (2017). Amyloid-independent atrophy patterns predict time to progression to dementia in mild cognitive impairment. Alzheimer's Research & Therapy, 9, 73.
+ Open protocol
+ Expand
7

CSF Biomarkers for Alzheimer's Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Collection of CSF by lumbar puncture for all subjects was performed as described previously (Van Der Flier et al., 2014). Aβ1–42, phosphorylated tau (p‐tau), and total tau protein concentrations were measured using InnoTest sandwich ELISAs (Innogenetics, Fujirebio, Ghent, Belgium). The cut‐off for abnormal drift‐corrected CSF Aβ1–42 was set at <813 ng/L (Tijms et al., 2018), at >375 ng/L for CSF total tau and at >52 ng/L for p‐tau (Mulder et al., 2010).
Pelkmans W., Dicks E., Barkhof F., Vrenken H., Scheltens P., van der Flier W.M, & Tijms B.M. (2019). Gray matter T1‐w/T2‐w ratios are higher in Alzheimer's disease. Human Brain Mapping, 40(13), 3900-3909.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!