Evos m7000 imaging system

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany

The EVOS M7000 Imaging System is a high-performance fluorescence microscope designed for imaging a wide range of biological samples. It features advanced optics, LED illumination, and a user-friendly software interface to capture and analyze images.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

EVOS M7000 (107 citations) EVOS imaging system (62 citations) EVOS M7000 cell imaging system (4 citations) EVOS M7000 imaging (3 citations) Evos M7000 life cell imaging system (2 citations) EVOS M7000 fluorescent imaging system (2 citations)

104 protocols using evos m7000 imaging system

Histological and Immunofluorescence Analysis of Mouse Optic Nerve

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were euthanized on Day 15. Both eyes of each mouse were enucleated and rapidly frozen in embedding medium prior to sectioning. The optic nerve parallel to the sagittal plane at the laser photocoagulation position was selected, and slices with a thickness of 4.0 um were prepared continuously. The sections were stained with HE, observed, and photographed using a light microscope (EVOS M7000 Imaging System, Thermo Fisher, Waltham, MA, USA).
Cryosections for immunofluorescence staining were thawed, air-dried, and fixed in 10% paraformaldehyde with 0.3% TritonX-100 at room temperature for 1 h. Immuno-fluorescence staining was performed according to the manufacturer’s instructions. Next, the sections were washed in PBS 3 times for 5 min. Then, the sections were incubated with pVEGFR primary antibodies (#3817, Cell Signaling, Danvers, MA, USA) at a 1:100 dilution overnight at 4 °C. The slides were washed with PBS 3 times for 5 min and incubated with an Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) secondary antibodies (#8889, Cell Signaling, Danvers, MA, USA) at 1:500 dilution for 2 h at room temperature. The nuclei were stained with 100 ng/mL DAPI for 10 min. The sections were washed with PBS between these incubations. Finally, the sections were mounted and examined and captured with a fluorescence microscope (EVOS M7000 Imaging System, Thermo Fisher, Waltham, MA, USA).

Liang I.C., Ko W.C., Hsu Y.J., Lin Y.R., Chang Y.H., Zong X.H., Lai P.C., Chang D.C, & Hung C.F. (2021). The Anti-Inflammatory Effect of Hydrogen Gas Inhalation and Its Influence on Laser-Induced Choroidal Neovascularization in a Mouse Model of Neovascular Age-Related Macular Degeneration. International Journal of Molecular Sciences, 22(21), 12049.

+ Open protocol

+ Expand

Immunofluorescence Staining of Brain Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sections were permeabilized in 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), blocked in CAS-Block^TM Histochemical Reagent (Thermo Fisher Scientific, Waltham, MA, USA), and then stained with the following antibodies: polyclonal rabbit anti-calbindin (1:500, CB38, Swant, Switzerland) and monoclonal mouse anti-fetuin-A (diluted 1:100, Santa Cruz Biotechnology, Dallas, TX, USA). Sections were then incubated with donkey anti-mouse IgG (H + L) Alexa Fluor 488 or donkey anti-rabbit IgG (H + L) Alexa Fluor 594 (1:300, Invitrogen, Carlsbad, CA, USA) for 2 h at room temperature. Fluoroshield^TM with DAPI (Sigma-Aldrich, St. Louis, MO, USA) was used for nuclear staining. Stained brain sections were analyzed with an immunofluorescence microscope (Thermo Fisher Scientific, Invitrogen^TM EVOS^TM M7000 Imaging System, USA). These stained brain sections were quantified with the ImageJ software v1.52a (Bethesda, MD, USA).
Cells were plated onto sterilized glass coverslips placed in 6- or 24-well culture plates. Cells were fixed with 4% PFA, permeabilized with 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, USA), blocked with CAS-Block^TM Histochemical Reagent (ThermoFisher Scientific, Waltham, MA, USA), and stained as described above.

Yoon S., Boonpraman N., Kim C.Y., Moon J.S, & Yi S.S. (2023). Reduction of fetuin-A levels contributes to impairment of Purkinje cells in cerebella of patients with Parkinson’s disease. BMB Reports, 56(5), 308-313.

+ Open protocol

+ Expand

Calcium Imaging of GBM Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

GMB cells and neocortical slices with GBM cells, expressing GCaMP6f reporter, were imaged on EVOS TM M7000 Imaging System (Invitrogen) at a sampling rate of 3-5 Hz for 10 min. More details are given in the Supplementary Methods.

Kuliesiute U., Joseph K., Straehle J., Madapusi Ravi V., Kueckelhaus J., Kada Benotmane J., Zhang J., Vlachos A., Beck J., Schnell O., Neniskyte U, & Heiland D.H. (2023). Sialic acid metabolism orchestrates transcellular connectivity and signaling in glioblastoma. Neuro-oncology, 25(11).

+ Open protocol

+ Expand

Immunohistochemical Analysis of CD44 and TIPRL

Check if the same lab product or an alternative is used in the 5 most similar protocols

Xenografts or tissues were fixed with 4% paraformaldehyde and embedded with paraffin. Then, the sections were dewaxed in xylene and rehydrated in alcohol. Antigen retrieval was performed using sodium citrate, and then endogenous peroxidase was blocked. The sections were incubated with specific rabbit anti-human CD44 (1:100, Boster Biological Technology, China), and TIPRL (1:200, Abcam, USA) at 4 °C overnight. After incubation with secondary antibodies at 37 °C for 1 h, DAB was used for detection. The sections were observed under an EVOSTM M7000 Imaging System (Thermo Fisher Scientific, USA).

Liang D.M., Li Y.J., Zhang J.X., Shen H.H., Wu C.X., Xie N., Liang Y., Li Y.M., Xue J.N., Sun H.F., Wang Q., Yang J., Li X.H., Wang P.Y, & Xie S.Y. (2024). m6A-methylated KCTD21-AS1 regulates macrophage phagocytosis through CD47 and cell autophagy through TIPR. Communications Biology, 7, 215.

+ Open protocol

+ Expand

Evaluating Xenograft Vascular Density

Check if the same lab product or an alternative is used in the 5 most similar protocols

Xenografts were fixed with 4% paraformaldehyde and embedded in paraffin. Immunohistochemistry (IHC) was performed on 3 μm sections to visualize vascular endothelium by using CD31 antibody (1:100; catalog: ab222783; Abcam), which could only react with mice antigen. Three field images were collected from each slide by using an EVOSTM M7000 Imaging System (Thermo Fisher Scientific, Inc.). The discrete cluster cells stained CD31 positive was counted down as one vessel. Tumor vessel density was reported as the average number of vessels number per field.
Hematoxylin and eosin (HE) staining was used to evaluate the morphological structure of nude mice injected with MSA.³⁴

Zhang Y., Wang R., Liu R., Xie S., Jiao F., Li Y., Xin J., Zhang H., Wang Z, & Yan Y. (2023). Delivery of miR‐3529‐3p using MnO2‐SiO2‐APTES nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting HIGD1A. Thoracic Cancer, 14(10), 913-928.

+ Open protocol

+ Expand

Microfluidic Cell Chip Analysis Platform

Check if the same lab product or an alternative is used in the 5 most similar protocols

The microfluidic cell chip culture analysis platform CellASIC ONIX2 was purchased from Merck & Co., Inc. (Darmstadt, Germany); the EVOS M7000 imaging system was purchased from Invitrogen Life Technology Co., Ltd. (Carlsbad, CA, USA); the research-grade fluorescence inverted microscope ECLIPSE TS100 was purchased from Nikon Corporation (Nikon, Japan); the Allegra x-22R Centrifuge was purchased from Beckman coulter, Inc. (Brea, CA, USA); the MCO-18AIC CO₂ Incubator was purchased from Panasonic Corporation (Tokyo, Japan); and the SpectraMax I3X Enzyme marker was purchased from Molecular Devices Instruments Ltd. (San Jose, CA, USA).

Chen D., Yin J., Huo J., Sun J., Huang J., Li T., Sun C., Yang Z, & Qin W. (2022). Optimization and Application of A Bionic System of Dynamic Co-Culture with Hepatocytes and Renal Cells Based on Microfluidic Chip Technique in Evaluating Materials of Health Food. Nutrients, 14(22), 4728.

+ Open protocol

+ Expand

Oxygen-induced retinopathy in transgenic mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Under approved IACUC protocol 16-09005, wild-type C57B6, or HtrA-1 over-expression transgenic mice (HtrA-1Tg; Nakayama et al., 2014 (link)) were placed in the oxygen-induced retinopathy (OIR) model of ROP (Stahl et al., 2010 (link); Iejima et al., 2015b (link)). A minimum of four biologic replicates, representing eight eyes, were performed for each condition: HtrA-1Tg RA; HtrA-1Tg OIR; C57B6 RA; C57B6 OIR. Two technical replicates were performed for each condition. Consistent with the OIR protocol as published (Connor et al., 2009 (link)), at p17 the mice were humanely euthanized and immediately following globes enucleated and fixed in 4% PFA in PBS. Retinas were isolated from fixed globes using a dissection microscope. The vasculature was then stained using Isolectin-594 and imaged in flat-mount preparation. Retinal images were obtained using an Invitrogen EVOS M7000 Imaging System. Quantification of neovascularization was done as described (Connor et al., 2009 (link)). In brief, we performed masked tracings of the neovascular retina, avascular retina, and total retinal area such that areas of neovascularization were quantified relative to the total retinal area for comparison between conditions (Connor et al., 2009 (link)).

Owen L.A., Shirer K., Collazo S.A., Szczotka K., Baker S., Wood B., Carroll L., Haaland B., Iwata T., Katikaneni L.D, & DeAngelis M.M. (2020). The Serine Protease HTRA-1 Is a Biomarker for ROP and Mediates Retinal Neovascularization. Frontiers in Molecular Neuroscience, 13, 605918.

+ Open protocol

+ Expand

H&E Staining with Tissue Preservation

Check if the same lab product or an alternative is used in the 5 most similar protocols

H&E staining (Abeam pic., Cambridge, UK) was performed at ~20 °C (room temperature) as previously described, with minor alterations to the previous procedure to avoid damage to the glue and tape⁷³. In brief, the tissue was incubated in hematoxylin, rinsed in water, incubated in bluing reagent, rinsed, and incubated in Eosin for contrast. Tissue was then coversliped with glycerol wet mount medium (Rs’ Science) for microscopic imaging using an EVOS^™ M7000 Imaging System (brightfield, 20x objective; Invitrogen, Waltham, MA) was used for microscopy imaging.

Bender K.J., Wang Y., Zhai C.Y., Saenz Z., Wang A, & Neumann E.K. (2023). Spatial lipidomics of fresh-frozen spines. bioRxiv.

+ Open protocol

+ Expand

Quantifying Basal Lipid ROS in Neural Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Basal lipid ROS levels in neural stem cells (NSCs) were measured using Liperfluo dye according to the manufacturer instructions (Dojindo Molecular Technologies, Inc, Rockville, MD USA). Briefly, the neurospheres were dissociated to single cells by Accutase (Sigma-Aldrich Inc., St. Louis, MO USA) treatment and seeded on Poly-D-Lysine coated plates at a density of 10⁵/cm². The cells were incubated in a humidified atmosphere of 95% air and 5% CO₂ at 37 °C and fed with NSC media till they reached proper confluency. Finally, these cells were incubated at 37 °C for 30 min with 10uM final concentration of Liperfluo dye (dissolved in DMSO) and images were taken using EVOS M7000 Imaging System (Invitrogen, Carlsbad CA, USA) coupled with EVOS onstage incubator for precise control of temperature, CO₂, and humidity. Images were taken at 20× objective using a GFP LED cube. Quantification of Liperfluo intensity was done by multiplying fluorescence intensity mean X% area from each group via ImageJ 1.52a (NIH, USA) and represented as relative fluorescence units (arbitrary units). For flow cytometric analysis, the cells were incubated with 10uM final concentration of Liperfluo dye at 37 °C for 30 min and washed with 1×PBS. Finally, the cells were collected in PBS transferred to flow acquisition tubes and quantified using BDLSR II (BD Biosciences, San Jose, CA, USA).

Dar N.J., Na R, & Ran Q. (2022). Functional Deficits of 5×FAD Neural Stem Cells Are Ameliorated by Glutathione Peroxidase 4. Cells, 11(11), 1770.

+ Open protocol

+ Expand

Quantifying Apoptosis with TUNEL Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed with 4% paraformaldehyde for 30 min at room temperature and washed twice with PBS. The cells were then incubated in the TUNEL solution containing FITC-dUTP for 60 min at 37 °C according to the manufacturer’s protocol (#C1088, Beyotime Biotechnology, China). Next, the cells were incubated in Hoechst 33342 for counterstaining of cell nuclei. Images were captured and analyzed using the EVOS M7000 Imaging System (Invitrogen, Thermo Fisher Scientific). The apoptosis rate was calculated as the ratio of FITC-positive cells (green cells) to total Hoechst 33342-positive cells (blue cells).

Shi Q., Sun B., Wang D., Zhu Y., Zhao X., Yang X, & Zhang Y. (2020). Circ6401, a novel circular RNA, is implicated in repair of the damaged endometrium by Wharton’s jelly-derived mesenchymal stem cells through regulation of the miR-29b-1-5p/RAP1B axis. Stem Cell Research & Therapy, 11, 520.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!