FITC mouse anti-human CD19 (Cat: 560994), CD34 (Cat: 560942), CD45 (Cat: 560976), CD73 (Cat: 561254), and CD105 (Cat: 561443) and PE mouse anti-human CD90 (Cat: 555596) were purchased from BD Pharmingen (BD Biosciences Systems; San Jose, CA, USA). The rabbit antibodies against phosphorylated NF-κB p65 (p-p65, Ser536) (Cat: 3033) and NF-κB p65 (Cat: 8242) were purchased from Cell Signaling Technology (Beverly, MA, USA). The rabbit antibodies against p53 (Cat: 345567), phosphorylated p38 MAPK (p-p38, Thr180/Tyr182) (Cat: 310091), and phosphorylated JNK (p-JNK Thr183/Tyr185) (Cat: R26311) and the mouse antibodies against p38 MAPK (Cat: 200782) and JNK (Cat: 201001) were purchased from ZENBIO Inc. (Chengdu, China). The rabbit antibodies against p21 (Cat: 10355-1-AP) and the lamin B1 (Cat: 12987-1-AP) polyclonal antibodies were purchased from Proteintech Group (Chicago, IL, USA). The polyclonal rabbit antibody against EV71 VP1 (Cat: GTX132339) was purchased from GeneTex, Inc. (Alton Pkwy Irvine, CA, USA). The rabbit antibody against EV71 3D (Cat: A8608) was purchased from Abclonal (Wuhan, China). The hydrogen peroxide (H2O2) was purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China).
Fitc mouse anti human cd19
Manufactured by BD
Sourced in United States
The FITC mouse anti-human CD19 is a laboratory reagent used to detect and identify CD19-positive cells in human samples. It is conjugated with the fluorescent dye FITC, allowing for the visualization and analysis of CD19-expressing cells through flow cytometry or other fluorescence-based techniques. This product provides a tool for researchers to study and characterize CD19-positive cells, which are important markers for B-cell development and function.
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Lab products found in correlation
Hydrogen peroxide h2o2, by Guangzhou Chemical (3 mentions)
Pe cy5 mouse anti human cd5, by BD (2 mentions)
Pe mouse anti human cd90, by BD (1 mentions)
Nf κb p65, by Cell Signaling Technology (1 mentions)
Cd105, by BD (1 mentions)
Cd38 pe, by BD (1 mentions)
Pe mouse anti human hla abc, by BD (1 mentions)
Facsdiva software version 7.0, by BD (1 mentions)
Alexa fluor 546 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Clone mopc 31c, by BD (1 mentions)
Pe mouse igg1, by BD (1 mentions)
Fitc mouse anti human cd3, by BD (1 mentions)
Mouse anti human cd4 pe, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Cd38 fitc, by BD (1 mentions)
Anti zap 70 pe, by BD (1 mentions)
6 protocols using fitc mouse anti human cd19
1
Characterization of EV71 Infection in Cells
2
CLL Cell Phenotyping: CD38 and ZAP-70
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In each case CLL cells were analyzed for CD38 antigen and ZAP-70 protein expression (as described previously [21 (link)]). Fresh PB samples were stained with the following set of monoclonal antibodies: FITC mouse anti-human CD19 (Clone SJ25C1, Cat No.: 340409), PE-Cy5 mouse anti-human CD5 (Clone UCHT2, Cat No.: 555354) and CD38 PE (Clone HIT2, Cat No.: 555460) or anti-ZAP-70 PE (Clone Clone 1E7.2, Cat No.: 344636) (BD Biosciences, Franklin Lakes, NJ, USA). Cells were considered positive for ZAP-70 with a cut-off point of ≥20%, and positive for CD38 expression with a cut-off point ≥30%.
3
Characterizing PBMC T and B Cells
4
Multicolor Flow Cytometry Immunophenotyping
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PE-Cy7 mouse anti-human CD3 (Clone UCHT1), APC mouse anti-human CD4 (Clone RPA-T4), FITC mouse anti-human CD14 (Clone MφP9), FITC mouse anti-human CD19 (Clone HIB19), PECY5 mouse anti-human CD11c (Clone B-ly6), APC mouse IgG2b isotype control (Clone 27–35), APC-Cy7 mouse IgG2a isotype Control (Clone G155–178), FITC mouse IgG2b isotype control (Clone MPC-11) and purified mouse IgG1 isotype control (Clone MOPC-31C) were purchased from BD (San Jose, CA, USA), whereas APC/CY7 mouse anti-human CD8 (clone RFT8) was from Southern Biothech (Birmingham, AL, USA), PECy7 mouse IgG1 isotype control and goat anti-mouse IgG Alexa Fluor 546 were from Invitrogen (Invitrogen, Thermo Fisher Scientific, Rockford, IL, USA), PECY5 IgG2a isotype Control (Clone eBM2a) was from eBioscience (eBioscience, Thermo Fisher Scientific), and mouse anti-flavivirus (clone 4G2) monoclonal antibody was kindly provided by Biomanguinhos (Fiocruz, Rio de Janeiro, Brazil). The 4G2 antibody recognizes a conserved epitope in the envelope protein from flaviviruses, including ZIKV [15 (link)].
5
Lymphocyte Phenotyping by Flow Cytometry
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At days 2, 4, 8, the lymphocytes suspension were collected by concentrated at 1000 revolutions/min for 10 min, then the precipitate was resuspended with 1 ml 0.9% physiological saline, after centrifuged at 1000 revolutions/min for 10 min, the precipitate was resuspended with 150 μl 0.9% physiological saline, and divided into three groups. One group as isotype control was added FITC mouse IgG2α (5 μl, BD), PE mouse IgG1 (5 μl, BD) and PerCP-CyTM 5.5 mouse IgG1 (1 μl, BD), the experiment group was divided into two group and, respectively, added FITC mouse anti-human CD3 (5 μl, BD), PE mouse anti-human CD4 (5 μl, BD), PerCP-CyTM5.5 mouse anti-human CD8 (1 μl, BD) and FITC mouse anti-human CD19 (5 μl, BD), PE mouse anti-human CD138 (5 μl, BD). The three groups were all incubated at room temperature for 15 min, then resuspended with 1 ml 0.9% physiological saline and centrifuged at 1000 revolutions/min for 10 min. Finally, the precipitate was resuspended with 0.2 ml 0.9% physiological saline and prepared to analysis using BD FACSCanto II. The experiment was repeated for 3 times.
6
CLL Immunophenotyping and FISH Analysis
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CLL cells were stained for CD38 antigen and ZAP-70 protein expression (as described previously [28] (link)). Fresh PB samples were stained with FITC mouse anti-human CD19 (Clone SJ25C1), PE-Cy5 mouse anti-human CD5 (Clone UCHT2) and CD38 FITC (Clone HIT2) or anti-ZAP-70 PE (Clone 1E7.2) (BD Biosciences). A cut-off point for ZAP-70 positivity in leukemic cells was ≥ 20%. Patients with CD38 expression lower or higher than 30% were classified as CD38 negative or positive, respectively.
I-FISH analysis. Detection of del(17p13.1) and del(11q22.3) was a part of the routine diagnostic practice. We used a previously described method [29] (link).
I-FISH analysis. Detection of del(17p13.1) and del(11q22.3) was a part of the routine diagnostic practice. We used a previously described method [29] (link).
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