Mice were injected intraperitoneally with a single dose of LPS or normal saline control. LPS was obtained from Sigma-Aldrich (catalog no. L4524, lot no. 107M4048V). Doses used were 12 μg (for cytokine measurement) or 15 μg (for survival study) of LPS per gram of body weight. For cytokine measurement, LPS was injected at 10 a.m., and plasma was collected 24 hours after injection. Plasma cytokine concentration was measured by Eve Technologies using the Mouse Cytokine Array/Chemokine Array 31-Plex (MD31). For the survival studies, mice were observed every 12 hours after LPS injection. We recorded the number of mice that died or reached the standard prespecified criteria for humane euthanasia in our IACUC protocol. For LPS challenge in the setting of AAV8 administration, mice were injected with 5 × 1011 VG of AAV8-mSerpina6 or empty virus control (EV) via tail vein. Six weeks after tail vein injection with virus, mice were injected intraperitoneally with 15 μg of LPS per gram of body weight, and survival was recorded as above.
Mouse cytokine array chemokine array 31 plex
Manufactured by Eve Technologies
Sourced in Canada
The Mouse Cytokine Array/Chemokine Array 31-Plex is a multiplex assay that can simultaneously detect and quantify the levels of 31 different mouse cytokines and chemokines in a single sample. The assay uses bead-based technology and is designed for use with a flow cytometer or Luminex instrument.
Automatically generated - may contain errors
Lab products found in correlation
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Bioplex 200, by Eve Technologies (1 mentions)
Elisa kit, by Thermo Fisher Scientific (1 mentions)
Bio plex mouse cytokine 23 plex, by Bio-Rad (1 mentions)
Interleukin 6 (il 6), by BioLegend (1 mentions)
Cxcl1, by R&D Systems (1 mentions)
Recombinant murine tnf, by Thermo Fisher Scientific (1 mentions)
19 protocols using mouse cytokine array chemokine array 31 plex
1
LPS-Induced Cytokine Response and Survival in Mice
2
Multiplex Cytokine Profiling of Mouse Serum
3
Multiplex Cytokine Analysis of Mouse Serum
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Mouse serum was analyzed using multiplex immunoassays designed for mice (Mouse Cytokine Array / Chemokine Array 31-Plex (MD31) from Eve Technologies), with 8 replicates from each group. Heatmaps display relative cytokine expression values normalized to vehicle-treated samples. Serum HMGB1 was analyzed by ELISA (NOVUS, NBP2–62767) according to the manufacturer’s protocol.
4
Macrophage Cytokine Profiling with Inhibitors
5
Macrophage Cytokine Profiling with Inhibitors
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Cytokine profiles for macrophage response to LPS/IFNγ with inhibitory drug treatments were probed by multiplexed analyte analysis (Mouse Cytokine Array/Chemokine Array 31-Plex (MD31), Eve Technologies, Calgary, CAN).
6
Comprehensive Biomarker Assessment
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Plasma corticosterone was quantified with a kit from ENZO Life Sciences. Cytokines (IL-6 and TNF-α) were quantified with corresponding ELISA kits from eBioscience. Plasma cytokines were also analyzed by Eve Technologies using Mouse Cytokine Array/Chemokine Array 31-Plex (MD31). The serum alanine aminotransferase (ALT) levels were quantified with a kit from POINTE SCIENTIFIC, Inc to determine the degree of liver damage. The blood urea nitrogen (BUN) concentrations were measured with a kit from the QuantiChrom to determine the degree of kidney injury.
7
Cytokine Profiling of Neonatal Lungs
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Cytokine protein levels in BAL supernatants were measured using Mouse Cytokine Array/Chemokine Array 31-Plex (MD31; Eve Technologies). GM-CSF protein levels were measured by ELISA (capture/detection antibodies from BioLegend). Lung pieces from PND3 pups were digested in Tris-NaCl-Tween buffer, and supernatants were quantitated by Bradford assay. Equal amounts of protein (2 mg) were tested by ELISA.
8
Serum Cytokine Profiling Protocol
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Serum was isolated and shipped to Eve Technologies for cytokine analysis using the Mouse Cytokine Array/Chemokine Array 31-Plex (MD31). To enable statistical analyses, values reported as out of range below the 4 or 5 parameter logistic standard curve were inputted as 0.02 pg/mL, the lowest extrapolated value that can be calculated by the standard curve mathematical formula.
9
Cytokine Profiling in Murine Trypanosomiasis
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Blood was collected from mice either non-infected or infected with either T. congolense 1/148 or IL3000 at the first peak of parasitemia by cardiac puncture. Blood was allowed to clot for 30 min at room temperature and then centrifuged at 1000 x g for 10 min at 4 °C. Serum was collected from each sample and added to an equal volume of PBS (pH 7.4). Samples were immediately frozen at –80 °C, and shipped in dry ice to Eve Technologies (Canada), where a Mouse Cytokine Array / Chemokine Array 31-Plex was performed, in duplicate.
10
Cytokine and Chemokine Profiling
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Cytokines and chemokines were analyzed by Mouse Discovery Assay (Eve Technologies, Mouse Cytokine Array/Chemokine Array 31-Plex). For each sample, conditioned media was collected as a supernatant of the media from bone and cancer cell cultures and processed by Eve Technologies according to the company’s specifications and requirements. Some samples measured zero, and some samples were considered out of range and not detectable. The out-of-range samples were given the value of zero for the analysis.
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