Gl 183

Manufactured by Beckman Coulter

Sourced in France

The GL-183 is a benchtop automated cell counter designed for the enumeration and viability analysis of various cell types. It utilizes advanced optical technology to provide accurate and reliable cell counting results. The core function of the GL-183 is to enable users to efficiently and accurately determine cell concentrations and viability in their samples.

Automatically generated - may contain errors

Lab products found in correlation

Fes172, by Beckman Coulter (3 mentions) Anti cd56 pc7, by Beckman Coulter (1 mentions) Anti cd3 pe cf594, by BD (1 mentions) Pei transfection reagent, by Polyplus Transfection (1 mentions) Anti kir2ds4, by Beckman Coulter (1 mentions) Anti igm pe, by Southern Biotech (1 mentions) Anti igg pe, by Southern Biotech (1 mentions) Facscalibur, by BD (1 mentions) Cellquest, by BD (1 mentions) Ncam16, by BD (1 mentions) Live dead fixable dead cell stain kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using gl 183

Production and Characterization of Monoclonal Antibodies

Cited 1 time

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The following primary mAbs were produced in our laboratories: M5A10 (IgG1, anti-PVR), L14 (IgG2a, anti-nectin-2), BAM195 (IgG1, anti-MICA), A6136 (IgM, anti-HLA class-I), NE97 (IgG2b, anti-B7-H3) (25 (link)), BAB281 (IgG1, anti-NKp46) (8 (link)). Anti-PD-L1.3.1 (IgG1, anti-PD-L1), and anti-PD-L2 (IgG1) were produced in D. Olive lab. D1.12 (IgG2a anti-HLA-II) mAb was produced by R. Accolla (University of Insubria, Varese, Italy). Anti-ULBP1-4 were purchased from R&D System (Minneapolis, US) and Santa Cruz Biotecnology (Dallas, US); anti-GD₂ (IgG2a) was purchased from BD Bioscience PharMingen, San Diego, CA); anti-CXCR4 (IgG2b) was purchased from &D Systems (Minneapolis, MN).
The following fluorochrome-conjugated anti-KIR in combination with anti-CD3-PECF594 (IgG1; BD Bioscience) and anti-CD56-PC7 (IgG1; Beckman Coulter, Marseille, France) mAbs were used: GL-183 (IgG1, anti-KIR2DL2/L3/S2; Beckman Coulter), EB6B (IgG1, anti-KIR2DL1/S1 and KIR2DL3*005; Beckman Coulter), Z27 (IgG1, anti-KIR3DL1/S1; Beckman Coulter), DX9 (IgG1, anti KIR3DL1; Miltenyi Biotec, Bergisch Gladbach, Germany), FES172 (IgG2a, KIR2DS4; Beckman Coulter), 143211 (IgG1, anti-KIR2DL1 and KIR2DS5; R&D). ECM41 mAb (IgM, anti-KIR2DL3, with the exception of *005; Our laboratory) followed by FITC-conjugated goat anti-mouse IgM (Southern Biotech, Birmingham, AL) was also used.

Marrella A., Dondero A., Aiello M., Casu B., Olive D., Regis S., Bottino C., Pende D., Meazza R., Caluori G., Castriconi R, & Scaglione S. (2019). Cell-Laden Hydrogel as a Clinical-Relevant 3D Model for Analyzing Neuroblastoma Growth, Immunophenotype, and Susceptibility to Therapies. Frontiers in Immunology, 10, 1876.

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Transient Transfection of HEK-293T Cells with KIR Alleles

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HEK‐293T cells were transiently transfected using the linear polyethylenimine derivative jetPEI transfection reagent (Polyplus, New York, NY) following the manufacturer's instruction. The pcDNA 3.1 plasmids used in the study code for: 2DL1*002, *003, *004, *012; 2DL2*001; 2DL3*002, *005; 2DS1*001, *002; 2DS2*001, 2DS4*001, and 2DS5*002. Forty‐eight hours after the transfection, the cells were tested with a panel of anti‐KIR2D mAb followed by anti‐IgG‐PE or anti‐IgM‐PE (Southern Biotechnology) by immunofluorescence and flow cytometric analysis (FACSCalibur and Cell Quest software, BD Biosciences). The mAbs used in these experiments were: 143211 (R&D Systems, Minneapolis, MN), 11PB6 (anti‐KIR2DL1/S1 and anti‐KIR2DL3 allotypes carrying E35 and R50, Miltenyi Biotec, Bergisch Gladbach, Germany), HP‐MA4 (anti‐KIR2DL1/S1/S3/S5, Biolegend, San Diego, CA), GL‐183 (anti‐KIR2DL2/L3/S2, Beckman Coulter), and FES172 (anti‐KIR2DS4, Beckman Coulter).

Falco M., Meazza R., Alicata C., Canevali P., Muntasell A., Bottino C., Moretta L., Pende D, & Lopez‐Botet M. (2022). Epitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes. Hla, 100(2), 107-118.

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Multiparameter Flow Cytometry of NK Cells

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Fluorescently labeled antibodies to CD56 (NCAM16.2, BD Biosciences), CD3 (SK7 or UCHT1, BD Biosciences), KIR2DL1/2DS1 (EB6B, Beckman Coulter), KIR2DL2/2DL3/2DS2 (GL183, Beckman Coulter or DX27, Biolegend), KIR2DS4 (FES172, Beckman Coulter), KIR2DL4 (mAb33, Biolegend), KIR3DL1 (DX9, BD Biosciences), KIR3DL2 (539304, R&D Systems), and NKG2A (Z199, Beckman Coulter) were used. Dead cells were excluded from the analysis using LIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen). Binding of human IgG to transfectants was determined using PE-labeled anti-human IgG F(ab)2 donkey fragments (Jackson ImmunoResearch).

Segerberg F., Lundtoft C., Reid S., Hjorton K., Leonard D., Nordmark G., Carlsten M, & Hagberg N. (2019). Autoantibodies to Killer Cell Immunoglobulin-Like Receptors in Patients With Systemic Lupus Erythematosus Induce Natural Killer Cell Hyporesponsiveness. Frontiers in Immunology, 10, 2164.

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