Anti caspase 1 sc 515

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-caspase-1 (sc-515) is a laboratory reagent used in research applications. It is an antibody that specifically recognizes and binds to the caspase-1 protein. Caspase-1 is an enzyme involved in various cellular processes. This product can be used to detect and study the expression and localization of caspase-1 in biological samples.

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Lab products found in correlation

Anti il 18 antibody ab137664, by Abcam (1 mentions) Anti il 6 antibody ab32530, by Abcam (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by American Type Culture Collection (1 mentions) Human ifnγ 285 if 100, by R&D Systems (1 mentions) Anti gapdh ab9484, by Abcam (1 mentions) Disuccinimidyl suberate dss 21655, by Thermo Fisher Scientific (1 mentions)

3 protocols using anti caspase 1 sc 515

Investigating Inflammatory Cytokine Regulation

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ARPE-19 cells (CRL-2302), THP-1 cells (TIB-202), and RPMI-1640 medium (30–2001) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA). Human primary retinal pigment epithelial cells (hRPE) (194987) were obtained from Lonza (Walkersville, MD). Dulbecco’s Modified Eagle Medium (DMEM/F-12) (11330-057), heat-inactivated fetal bovine serum (HI-FBS) (10438-026) and Penicillin-Streptomycin (15140122) were purchased from Life Technologies (Grand Island, NY). HP X-tremeGene transfection agent (06366244001) was obtained from Roche (Indianapolis, IN), recombinant human IL-1α (200-LA-010) from R&D Systems (Minneapolis, MN), and Bay 11–7082 inhibitor (196870) from EMD Millipore (Billerica, MA). Anti-IL-18 antibody (ab137664), anti-IL-6 antibody (ab32530), and anti-TATA binding protein (TBP) (51841) were obtained from Abcam (Cambridge, MA). Anti-IL-1β antibody (MAB201), anti-IL-8 antibody (MAB208), and anti-STING (MAB7169) were purchased from R&D Systems. Anti-caspase-1 (sc-515) was purchased from Santa Cruz Biotechnology (Dallas, TX). Anti-β-tubulin antibody (2128), anti-A20 (5630) and HRP-linked secondary antibodies (7074S, 7076S) were obtained from Cell Signaling Technology (Danvers, MA). ELISA kits for IL-1β (DLB50), IL-18 (7620) and IL-8 (D8000C) were purchased from R&D. Taq Polymerase (M0267S) was purchased from New England Biolabs (Ipswich, MA).

Dib B., Lin H., Maidana D.E., Tian B., Miller J.B., Bouzika P., Miller J.W, & Vavvas D.G. (2015). Mitochondrial DNA has a pro-inflammatory role in AMD. Biochimica et biophysica acta, 1853(11 Pt A), 2897-2906.

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Western Blot Antibody Panel

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Anti-FLAG (2368S, CST, USA), anti-Caspase-1 (sc-515, Santa Cruz), anti-EGFP (Vatoz, Turkey), anti-ASC (kindly provided by Dr Masumoto, Jichi Medical University), anti-mCherry (kindly provided by Dr Arzu Celik, Bogazici University) were used for Western blotting.

Sahillioğlu A.C, & Özören N. (2015). Artificial Loading of ASC Specks with Cytosolic Antigens. PLoS ONE, 10(8), e0134912.

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Characterization of Sulfur Metabolism Proteins

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The following primary antibodies were used in this study: anti-SQOR (17256-1-AP) and anti-SLC7A11/xCT (26864-1-AP) from Proteintech, anti-caspase 1 (SC-515) from Santa Cruz Biotechnology, anti-NLRP3 (ag-20b-0014) and anti-ASC (AG-25B-0006) from AdipoGen, anti-IL-1β (5129-100) from Biovision, anti-GAPDH (ab9484) from Abcam. LPS from E. coli 055:B5 (L4005), PMA (P8139), dl-propargylglycine (p7888), aminooxyacetate (C13408) and sulfasalazine (S0883) were purchased from Sigma. Mouse IFN-γ (485-MI-100) and human IFN-γ (285-IF-100) were from R&D Systems. Poly (dA:dT) (tlrl-patn-1) and flagellin (tlrl-stfla) were from InvivoGen. EZ-Link Maleimide-PEG2-Biotin (21901BID), streptavidin-agarose resin (20361) and disuccinimidyl suberate (DSS) (21655) were from Thermo Scientific. The fluorescent probes WSP-5 and SSP4 were a kind gift of Prof. Ming Xian (Brown University). The MPST inhibitor I3-MT-3 was a kind gift of Prof. Kenjiro Hanaoka (Keio University, Tokyo, Japan). Tissue culture media and reagents were from Biological Industries (Beit Haemek, Israel).

Salti T., Braunstein I., Haimovich Y., Ziv T, & Benhar M. (2024). Widespread S-persulfidation in activated macrophages as a protective mechanism against oxidative-inflammatory stress. Redox Biology, 72, 103125.

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