Log In Sign up
The largest database of trusted experimental protocols

Fatty acid free bovine serum albumin fraction 5

Manufactured by Merck Group
Visit Supplier
Sourced in United States

Fatty acid-free bovine serum albumin (BSA) fraction V is a high-purity protein derived from bovine serum. It is a widely used component in cell culture media and various biomedical research applications.

Automatically generated - may contain errors

Lab products found in correlation

Trichloroacetic acid tca, by Merck Group (3 mentions) Thiobarbituric acid, by Merck Group (3 mentions) D mannitol, by Merck Group (3 mentions) Coomassie brilliant blue, by Merck Group (2 mentions) Ethylenediaminetetraacetic acid edta, by Merck Group (2 mentions) Ammonium chloride, by Merck Group (2 mentions) Hydroxymethyl aminomethane hydrochloride tris hcl, by Merck Group (2 mentions) Glutathione (gsh), by Merck Group (2 mentions) Malondialdehyde, by Merck Group (2 mentions) 3 n morpholino propanesulfonic acid, by Merck Group (2 mentions) Ferric chloride hexahydrate, by Merck Group (2 mentions) 2 7 dichlorofluorescein, by Merck Group (2 mentions) Metaphosphoric acid, by Merck Group (2 mentions) Dithiothreitol (dtt), by Merck Group (2 mentions) Sucrose, by Merck Group (2 mentions) N butanol, by Merck Group (2 mentions) 2 4 6 tripyridyl s triazine, by Merck Group (2 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Glycerol assay reagent, by Merck Group (2 mentions)

Spelling variants of this product

Bovine serum albumin fraction V (135 citations) Fatty acid-free bovine serum albumin (128 citations) Albumin Bovine Fraction V (18 citations) Fatty acid-free albumin (8 citations) -free bovine serum albumin (8 citations) Bovine fatty acid free albumin (4 citations) Fatty acid-free serum albumin (3 citations) Acid free bovine serum albumin (BSA) (2 citations) Bovine serum fraction V (2 citations) Bovine serum albumin V (2 citations)

8 protocols using fatty acid free bovine serum albumin fraction 5

1

TGF-β Signaling Pathway Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant TGF-β1 was obtained from PeproTech (Rocky Hill, NJ) and fatty acid–free bovine serum albumin (BSA; fraction V) from Sigma-Aldrich (St. Louis, MO). Media and cell culture reagents were from Biological Industries (Beit Haemek, Israel) or Invitrogen (Carlsbad, CA). Rabbit immunoglobulin G (IgG) against Smad3 (reactive with Smad3 and Smad2; sc-528) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). G418 was purchased from Calbiochem (La Jolla, CA). Dual-luciferase reporter (DLR) assay system was from Promega (Fitchburg, WI). Affinity-purified biotinylated goat anti-rabbit (GαR) IgG and Cy3-streptavidin were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA). Anti-myc tag (αmyc) 9E10 mouse ascites (Evan et al., 1985 (link)) and HA.11 rabbit antiserum to the HA tag (αHA) were from Covance Research Products (Denver, PA). IgG and monovalent Fab′ fragment αmyc were prepared from the 9E10 ascites as described (Henis et al., 1994 (link)). Alexa Fluor 488–GαR IgG and Alexa Fluor 546–goat anti-mouse (GαM) F(ab′)2 were from Invitrogen-Molecular Probes (Eugene, OR); fluorescent F(ab′)2 was converted into Fab′ as described (Gilboa et al., 1998 (link)). [125I]TGFβ-1 was from PerkinElmer (Waltham, MA). Goat IgG against the extracellular domain of TβRIII was from R&D Systems (Minneapolis, MN). Mouse anti–β-actin was from Sigma-Aldrich.
Tazat K., Hector-Greene M., Blobe G.C, & Henis Y.I. (2015). TβRIII independently binds type I and type II TGF-β receptors to inhibit TGF-β signaling. Molecular Biology of the Cell, 26(19), 3535-3545.
+ Open protocol
+ Expand
2

Oxidative Stress Biomarker Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
4,2-Hydroxyethyl,1-piperazine ethane sulfonic acid (HEPES), thiobarbituric acid (TBA), 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid (trolox), sucrose, fatty acid-free bovine serum albumin (BSA) fraction V, Coomassie brilliant blue, dithiobis-2-nitrobenzoic acid (DTNB), malondialdehyde (MDA), glutathione (GSH), 2′,7′-Dichlorofluorescein diacetate (DCFH-DA), potassium chloride (KCl), sodium phosphate dibasic (Na2HPO4), sodium phosphate monobasic (NaH2PO4), and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Ammonium chloride, Trichloroacetic acid (TCA), and hydroxymethyl aminomethane hydrochloride (Tris-HCl) were purchased from Merck (Darmstadt, Germany). Citicoline was purchased from Alborz Darou® (Tehran, Iran). Commercial kits were used for the assessment of plasma biomarkers (Pars Azmoon®, Tehran, Iran).
Farshad O., Keshavarz P., Heidari R., Farahmandnejad M., Azhdari S, & Jamshidzadeh A. (2020). The Potential Neuroprotective Role of Citicoline in Hepatic Encephalopathy. Journal of Experimental Pharmacology, 12, 517-527.
+ Open protocol
+ Expand
3

Antioxidant and Cytotoxicity Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
2,4,6-tripyridyl-s-triazine (TPTZ), 3-[4,5dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), 3-(N-morpholino) propane sulfonic acid (MOPS), Coomassie brilliant blue, 2’,7’-dichlorofluorescein (DCFH), 6-hydroxy-2,5,7,8-tetramethylchroman-carboxylic acid (Trolox), dithiothreitol (DTT), fatty acid-free bovine serum albumin (BSA) fraction V, ferric chloride hexahydrate (FeCl3•6H2O), D-mannitol, reduced glutathione (GSH), malondialdehyde (MDA), sodium succinate, rhodamine 123 (Rh 123), oxidized glutathione (GSSG), thiobarbituric acid (TBA), taurine, sucrose, and trichloroacetic acid (TCA) were purchased from Sigma (Sigma-Aldrich, St. Louis, MO). Kits for evaluating serum biochemistry were obtained from Pars Azmun (Tehran, Iran). Dimethyl sulfoxide (DMSO), 2-amino-2-hydroxymethyl-propane-1, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), dinitrofluorobenzene (DNFB), 3-diol-hydro-chloride (Tris-HCl), glacial acetic acid, ethylenediaminetetraacetic acid (EDTA), n-butanol, iodoacetic acid, and meta-phosphoric acid were obtained from Merck (Darmstadt, Germany).
Mousavi K., Niknahad H., Ghalamfarsa A., Mohammadi H., Azarpira N., Ommati M.M, & Heidari R. (2020). Taurine mitigates cirrhosis-associated heart injury through mitochondrial-dependent and antioxidative mechanisms. Clinical and Experimental Hepatology, 6(3), 207-219.
+ Open protocol
+ Expand
4

Oxidative Stress Biomarker Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sodium succinate, fatty acid-free bovine serum albumin (BSA) fraction V, thiobarbituric acid (TBA), 4,2-hydroxyethyl,1-piperazineethanesulfonic acid (HEPES), manganese chloride, dimethyl sulfoxide (DMSO), ammonium chloride, D-mannitol, 3-(N-morpholino)-propane sulfonic acid (MOPS), n-butanol, 3-[4,5dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), 2’,7’dichlorofluorescein (DCF), sucrose, rhodamine 123 (Rh 123), Coomassie brilliant blue, and ethylene glycol-bis-(2-amino-ethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Hydroxymethyl aminomethane hydrochloride (Tris-HCl) and trichloroacetic acid (TCA) were purchased from Merck (Darmstadt, Germany). All salts for preparing buffers were of the analytical grade and obtained from Merck (Darmstadt, Germany).
Heidari R., Jamshidzadeh A., Ommati M.M., Rashidi E., Khodaei F., Sadeghi A., Hosseini A, & Niknahad H. (2019). Ammonia-induced mitochondrial impairment is intensified by manganese co-exposure: relevance to the management of subclinical hepatic encephalopathy and cirrhosis-associated brain injury. Clinical and Experimental Hepatology, 5(2), 109-117.
+ Open protocol
+ Expand
5

Kynurenine Pathway Metabolites Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ANA, 3-OH-ANA, L-KYN, 3-OH-KYN, KYNA, L-TRP, QUIN, xanthurenic acid (XAN), L-malic acid, rotenone, NADH (disodium salt), decylubiquinone (2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone) and fatty acid-free bovine serum albumin (BSA, fraction V) came from Sigma-Aldrich. Triethanolamine-HCl, KCN, succinic acid and L-glutamic acid were purchased from Fluka. ADP (potassium salt) was obtained from Boehringer. Other chemicals were from Merck.
Baran H., Staniek K., Bertignol-Spörr M., Attam M., Kronsteiner C, & Kepplinger B. (2016). Effects of Various Kynurenine Metabolites on Respiratory Parameters of Rat Brain, Liver and Heart Mitochondria. International Journal of Tryptophan Research : IJTR, 9, 17-29.
+ Open protocol
+ Expand
6

Adipogenesis Induction by STZ and Insulin

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Carlsbad, CA). Streptozotocin (STZ) was obtained from Enzo Life Science (USA). Fatty acid-free bovine serum albumin fraction V, glycerol assay reagent, isoproterenol, penicillin-streptomycin, type-II collagenase, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt (HEPES) were provided from Sigma (USA). Dimethyl sulfoxide and 3-isobutyl-1-methylxanthine (IBMX) were purchased from Fluka Chemical Co. Indomethacin and human insulin were kindly provided by EXIR Company (Iran).
Ghorbani A., Moradi Marjaneh R., Rajaei Z, & Hadjzadeh M.A. (2014). Effects of Securigera securidaca Extract on Lipolysis and Adipogenesis in Diabetic Rats. Cholesterol, 2014, 582106.
+ Open protocol
+ Expand
7

Comprehensive Biochemical Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glutathione (GSH), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), trichloroacetic acid, malondialdehyde, 3-[4,5dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, 2,4,6-tripyridyl-s-triazine, ferric chloride hexahydrate (FeCl3.6H2O), D-mannitol, thiobarbituric acid, 3-(N-morpholino) propane sulfonic acid, fatty acid-free bovine serum albumin fraction V, coomassie brilliant blue, Rhodamine123, dinitrophenylhydrazine (DNPH), dithiothreitol (DTT), ethylene glycol-bis(β-aminoethyl ether)-N, N, N′, N′-tetraacetic acid (EGTA), Ethylenediaminetetraacetic acid (EDTA), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), tramadol, bovine serum albumin (BSA), and sucrose were obtained from Sigma (Sigma-Aldrich, St. Louis, MO). Kits for assessing biomarkers of renal injury were obtained from Parsazmoon® (Tehran, Iran). High-performance liquid chromatography (HPLC) grade methanol, potassium chloride (KCl), 3-(N-morpholino) propanesulfonic acid (MOPS), iodoacetic acid, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), acetonitrile HPLC grade, meta-phosphoric acid, dinitro fluoro benzene, n-butanol, and 2-amino-2-hydroxymethyl-propane-1,3-diol-hydrochloride (Tris-HCl), were purchased from Merck (Darmstadt, Germany).
Mousavi K., Manthari R.K., Najibi A., Jia Z., Ommati M.M, & Heidari R. (2021). Mitochondrial dysfunction and oxidative stress are involved in the mechanism of tramadol-induced renal injury. Current Research in Pharmacology and Drug Discovery, 2, 100049.
+ Open protocol
+ Expand
8

Isolation and Characterization of Human Adipocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
High glucose Dulbecco's Modified Eagles Medium (DMEM), fetal bovine serum (FBS), and trypsin were purchased from Gibco (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO), fatty acid-free bovine serum albumin fraction V, glycerol assay reagent, isoproterenol (ISO), human adiponectin enzyme-linked immunosorbent assay (ELISA) kit, penicillin-streptomycin solution, type-II collagenase, 3-isobutyl-1-methylxanthine, 3-(4,5dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt (HEPES) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Human C-peptide was purchased from Bachem (Bubendorf, Switzerland). Human insulin and indomethacin were kindly provided by Exir Company, Iran. Fluorescein isothiocyanate-conjugated antibodies against CD34, CD44, CD45, and CD105 were bought from AbD Serotec (Raleigh, NC, USA).
Ghorbani A., Shafiee-Nick R., Zojaji S.A, & Rajabi-Mashhadi M.T. (2017). Physiological effects of proinsulin-connecting peptide in human subcutaneous adipose tissue. Physiology international, 104(2).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!