Fmoc dab ivdde oh

Manufactured by Chem-Impex

Sourced in United States

Fmoc-Dab(ivDde)-OH is a protected amino acid used in peptide synthesis. It consists of a Fmoc-protected diaminobutyric acid (Dab) with an ivDde (1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)-3-methylbutyl) protecting group on the side chain.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using fmoc dab ivdde oh

Multistep Peptide Synthesis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Piperidine, diisopropylethylamine
(DIPEA), trifluoroacetic acid (TFA), and 1H-benzotriazolium-1-[bis(dimethylamino)methylene]-5-chloro-hexafluorophosphate-(1-),3-oxide
(HCTU) were obtained from Auspep (Melbourne, Australia). Fmoc-Dab(Boc)-OH,
Fmoc-d-Phe(4-Iodo)-OH, and Fmoc-Dab(ivDde)-OH were from Chem-Impex
International (U.S.A.). Fmoc-Thr(tBu)-OH was purchased from Mimotopes
(Melbourne, Australia). Fmoc-OctGly-OH was obtained from Try-lead
Chem (Hangzhou, China). Dimethylformamide (DMF), methanol (CH₃OH), diethyl ether, dichloromethane (DCM), hydrochloric acid,
and acetonitrile were from Merck (Melbourne, Australia). Fmoc-Thr(tBu)-TCP-resin
was obtained from Intavis Bioanalytical Instruments (Koeln, Germany).
Dansylglycine, triisopropylsilane (TIPS), and diphenylphosphorylazide
(DPPA) were from Sigma-Aldrich (Castle Hill, Australia).

Azad M.A., Roberts K.D., Yu H.H., Liu B., Schofield A.V., James S.A., Howard D.L., Nation R.L., Rogers K., de Jonge M.D., Thompson P.E., Fu J., Velkov T, & Li J. (2015). Significant Accumulation of Polymyxin in Single Renal Tubular Cells: A Medicinal Chemistry and Triple Correlative Microscopy Approach. Analytical Chemistry, 87(3), 1590-1595.

+ Open protocol

+ Expand

Synthesis of Polymyxin Lipopeptide Antibody

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-polymyxin B mouse IgM antibody (clone 45) was obtained from Thermo
Fisher Scientific (Rockford, Illinois, USA). Polymyxin B (sulfate), colistin
(sulfate), triisopropylsilane (TIPS), trifluoroacetic acid, (TFA) and
diphenylphosphorylazide (DPPA), Streptavidin were purchased from Sigma-Aldrich
(Sydney, New South Wales, Australia). Piperidine and diisopropylethylamine
(DIPEA) were obtained from Auspep (Melbourne, Victoria, Australia).
Fmoc-Dab(Boc)-OH was purchased from Try-lead Chem (Hangzhou, Zhejiang,China).
Fmoc-Thr(tBu)-OH and Fmoc-Leu-OH were from Mimotopes (Melbourne, Australia).
Fmoc-Dab(ivDde)-OH, Fmoc-D-Phe-OH, Fmoc-Dap-OH, Fmoc-D-Dap-OH and
1H-Benzotriazolium-1-[bis(dimethylamino)methylene]-5-chloro
hexafluoro-phosphate-(1-),3-oxide (HCTU) were purchased from Chem-Impex
International (Wood Dale, Illinois, USA). Fmoc-Thr(tBu)-TCP-Resin was obtained
from Intavis Bioanyltical Instruments (Köln, Cologne,Germany).
Dimethylformamide (DMF), methanol (MeOH), diethyl ether, dichloromethane (DCM),
hydrochloric acid and acetonitrile were purchased from Merck (Melbourne,
Victoria, Australia). Polymyxin lipopeptide stock solutions were prepared with
Milli-Q water (Millipore, North Ryde, New South Wales, Australia) and filtered
using 0.22-µm syringe filters (Sartorius, Melbourne, Victoria,
Australia). Solutions were stored at 4°C for up to one month.^{57 (link)}

Velkov T., Yun B., Schneider E.K., Azad M.A., Dolezal O., Morris F.C., Nation R.L., Wang J., Chen K., Yu H.H., Chen L., Thompson P.E., Roberts K.D, & Li J. (2016). A novel chemical biology approach for mapping of polymyxin-lipopeptide antibody binding epitopes. ACS infectious diseases, 2(5), 341-351.

+ Open protocol

+ Expand

Peptide Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Diisopropylethylamine (DIPEA) was obtained from Auspep (Melbourne, Australia). Fmoc-L-OctGly-OH and Fmoc-Dab(Boc)-OH were obtained from Try-lead Chem (Hangzhou, China). Fmoc-Dab(ivDde)-OH, Fmoc-D-Leu-OH, 1H-Benzotriazolium-1-[bis(dimethylamino)methylene]-5-chloro hexafluoro- phosphate-(1-),3-oxide (HCTU) and 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP) were obtained from Chem-Impex International (Wood Dale, IL, USA). Fmoc-Thr(tBu)-OH, Fmoc-Ala-OH and Fmoc-Gly-OH were obtained from Mimotopes (Melbourne, Australia). N-Fmoc-Amido-dPEG₂-OH was obtained from Peptides International (Louisville, KY, USA). Dichloromethane (DCM), dimethylformamide (DMF), diethyl ether and acetonitrile were obtained from Merck (Melbourne, Australia). Fmoc-Thr(tBu)-TCP-Resin was obtained from Intavis Bioanyltical Instruments (Köln, Germany). Piperidine, triisopropylsilane (TIPS), trifluoroacetic acid (TFA), dansyl-chloride, ethanedithiol (EDT) and diphenylphosphorylazide (DPPA) were obtained from Sigma-Aldrich (Castle Hill, Australia) Polymyxin B sulfate and colistin sulfate were research grade and obtained from BetaPharma (Shanghai, China).

Yun B., Roberts K.D., Thompson P.E., Nation R.L., Velkov T, & Li J. (2017). Design and Evaluation of Novel Polymyxin Fluorescent Probes. Sensors (Basel, Switzerland), 17(11), 2598.

+ Open protocol

+ Expand

Solid-Phase Synthesis of Polymyxin Peptides

Check if the same lab product or an alternative is used in the 5 most similar protocols

Polymyxin B (sulfate), colistin (sulfate),
lipopolysaccharides (LPS), dansylglycine, triisopropylsilane (TIPS),
diphenylphosphorylazide (DPPA), and diisopropylethylamine (DIPEA)
were obtained from Sigma-Aldrich (Sydney, NSW, Australia). All other
reagents were of the highest purity and commercially available. Stock
solutions of Polymyxin B and colistin were prepared in Milli-Q water
(Millipore, North Ryde, NSW, Australia) and filtered through 0.22-μm
syringe filters (Sartorius, Melbourne, Vic, Australia). Solutions
were stored at 4 °C for up to one month.^{8 (link)} Piperidine, diisopropylethylamine (DIPEA), trifluoroacetic acid
(TFA), and ¹H-benzotriazolium-1-[bis(dimethylamino)methylene]-5-chloro-hexafluorophosphate-(1)-3-oxide
(HCTU) were purchased from Auspep (Melbourne, Australia). Fmoc-Dab(Boc)-OH
and Fmoc-Dab(ivDde)-OH were obtained from Chem-Impex International
(USA). Fmoc-Thr(tBu)-OH was from Mimotopes (Melbourne, Australia).
Fmoc-OctGly-OH was obtained from Try-lead Chem (China). Dimethylformamide
(DMF), methanol (MeOH), diethyl ether, dichloromethane (DCM), hydrochloric
acid, and acetonitrile were obtained from Merck (Melbourne, VIC, Australia),
and Fmoc-Thr(tBu)-TCP-Resin from Intavis Bioanyltical Instruments,
Germany. Dodecylphosphocholine (purity >99%) was purchased from
Avanti
Polar Lipids (USA, Alabama).

Deris Z.Z., Swarbrick J.D., Roberts K.D., Azad M.A., Akter J., Horne A.S., Nation R.L., Rogers K.L., Thompson P.E., Velkov T, & Li J. (2014). Probing the Penetration of Antimicrobial Polymyxin Lipopeptides into Gram-Negative Bacteria. Bioconjugate Chemistry, 25(4), 750-760.

+ Open protocol

+ Expand

Synthesis of Peptides with Fmoc Chemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The general synthetic methodology used for the synthesis of the peptides has been previously described in detail elsewhere by our group.^{43 (link)} The materials and synthesis protocol used are also presented below.
Piperidine, diisopropylethylamine (DIPEA) and trifluoroacetic acid (TFA) were purchased from Auspep (Melbourne, Australia). Fmoc-Dab(Boc)-OH, was purchased from Try-lead Chem (Hangzhou, China). Fmoc-d-Phe-OH, Fmoc-Dab(ivDde)-OH, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and 1H-benzotriazolium-1-[bis(dimethylamino)methylene]-5-chloro hexafluoro phosphate-(1-),3-oxide (HCTU) were purchased from Chem-Impex International (Wood Dale, IL, USA). Fmoc-Ala-OH, Fmoc-Leu-OH and Fmoc-Thr(tBu)-OH were purchased from Mimotopes (Melbourne, Australia). Dimethylformamide (DMF), methanol (MeOH), diethyl ether, dichloromethane (DCM), hydrochloric acid (HCl) and acetonitrile were purchased from Merck (Melbourne, Australia). Fmoc-Thr(tBu)-TCP-Resin was purchased from Intavis Bioanayltical Instruments (Germany). Triisopropylsilane (TIPS), 1,2-ethanedithiol (EDT), diphenylphosphorylazide (DPPA) and hydrazine were obtained from Sigma-Aldrich (Castle Hill, Australia).

Jiang X., Patil N.A., Azad M.A., Wickremasinghe H., Yu H., Zhao J., Zhang X., Li M., Gong B., Wan L., Ma W., Thompson P.E., Yang K., Yuan B., Schreiber F., Wang L., Velkov T., Roberts K.D, & Li J. (2021). A novel chemical biology and computational approach to expedite the discovery of new-generation polymyxins against life-threatening Acinetobacter baumannii. Chemical Science, 12(36), 12211-12220.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!