Eclipse model ni u microscope

Manufactured by Nikon

Sourced in Japan

The Nikon Eclipse model Ni-U is a high-quality microscope designed for laboratory and research applications. It features a sturdy optical system, LED illumination, and a range of objectives to accommodate various sample types. The microscope is capable of providing clear and detailed images for a variety of applications, including materials science, life sciences, and quality control.

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Lab products found in correlation

Dako autostainer, by Agilent Technologies (2 mentions) Dako envision flex, by Agilent Technologies (2 mentions) Tissue tek prisma, by Sakura Finetek (1 mentions) Hematoxylin, by Merck Group (1 mentions)

Spelling variants of this product

Eclipse Ni (382 citations) Eclipse Ni-U (316 citations) Eclipse Ni microscope (214 citations) Eclipse microscope (191 citations) Ni-U microscope (44 citations) MODEL ECLIPSE NI-U (5 citations)

4 protocols using eclipse model ni u microscope

Immunohistochemical Analysis of CD3 and CD20

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For light microscopy, 4 μm thick sections from formalin-fixed paraffin-embedded tissue were automatically stained with hematoxylin and eosin in a Sakura Tissue-Tek Prisma instrument (Sakura Finetek, Torrance, California, USA). The immunostainings were done on a Dako Autostainer instrument (Dako, Agilent Technologies, Santa Clara, California, USA, model Link 48), and the incubation time for the primary antibodies was 20 min. CD3 was immunostained by clone SP7 (a monoclonal rabbit antibody, diluted 1:150; Thermo Scientific, Waltham, Massachusetts, USA; cat. no. RM-9107), and CD20 was immunostained by clone L26 (a mouse IgG2a antibody, diluted 1:600; Dako, cat. no. M0755). The secondary detection was performed with Dako EnVision
^TM Flex (Dako, cat. no. K8000) for 20 min, followed by diaminobenzidine (DAB) staining for 10 min. The slides were thereafter treated with CuSO4 for 5 min before contrastaining with hematoxylin. Samples were examined with a Nikon Eclipse model N
i-U microscope (Nikon, Tokyo, Japan) equipped with Nikon Plan-Fluor objective lenses (2×, 20×, and 40×) and images were taken with an Infinity 2 digital camera (Lumenera Corporation, Nepean, Ontario, Canada).

Joly-Battaglini A., Hammarström C., Stankovic B., Aamodt H., Stjärne J., Brustugun O.T., Helland Å., Øynebråten I, & Corthay A. (2016). Rituximab efficiently depletes B cells in lung tumors and normal lung tissue. F1000Research, 5, 38.

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Immunohistochemical Analysis of CD45 in Tumor Tissue

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Representative areas for the amount of inflammation in each tumor were chosen. The immunostaining was done on 2.5 μm thick FFPE tumor tissue using a Dako Autostainer instrument (Dako, Agilent Technologies, Santa Clara, California, USA, model Link 48). Epitope retrieval was performed with Dako Flex HpH according to the manufacturer's protocol (Dako EnVision FLEX, Cat-no: K8000). Tissue sections were incubated for 20 min with primary anti-CD45 monoclonal antibodies (clones 2B11 and PD7/26, diluted 1:300; Dako, Cat-no: M0701). The secondary detection was performed with Dako EnVision TM Flex (Dako, Cat-no: K8000) for 20 min, followed by diaminobenzidine (DAB) staining for 10 min (Dako EnVision FLEX, Cat-no. K800021-2). The slides were thereafter treated with 0.5% CuSO₄ for 5 min before counterstaining with hematoxylin (Merck, Cat-no: 1.15938.0100) to visualize cell nuclei. Tissue sections were examined with a Nikon Eclipse model N i-U microscope (Nikon, Tokyo, Japan) equipped with Nikon Plan-Fluor objective lenses (2×, 10×, 20×, 40×, and 60×) and images were taken with an Infinity 2 digital camera (Lumenera Corporation, Nepean, Ontario, Canada).

Stankovic B., Bjørhovde H.A., Skarshaug R., Aamodt H., Frafjord A., Müller E., Hammarström C., Beraki K., Bækkevold E.S., Woldbæk P.R., Helland Å., Brustugun O.T., Øynebråten I, & Corthay A. (2019). Immune Cell Composition in Human Non-small Cell Lung Cancer. Frontiers in Immunology, 9, 3101.

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Immunohistochemical Analysis of MDI Exposure

Cited 1 time

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Studies were performed on replicate samples of cytospun airway cells obtained by bronchoalveolar lavage (BAL) and lung tissue sections of MDI exposed or control mice acquired during prior studies as previously reported (Wisnewski, Liu et al. 2015 (link)). Cytospun BAL cells fixed with −20°C methanol and formalin-fixed paraffin-embedded tissue sections were blocked with 20% goat serum and stained with 2 μg/ml of KLH-depleted, protein-A affinity purified anti-MDI rabbit IgG, or 2 μg/ml of isotype control protein-A purified polyclonal rabbit IgG raised against IL-33 or 2 μg/ml protein-A purified normal rabbit IgG (not shown). Endogenous peroxidase was quenched before analysis using H₂O₂, and endogenous biotin was blocked before addition of biotin-labeled mouse monoclonal anti-rabbit IgG (with no cross-reactivity to other species IgG) using commercially available “biotin-blocking solution” (see Section 2.1 above). Slides were further developed with peroxidase-labeled streptavidin, DAB substrate, and hematoxylin counterstain. Micrographs were taken under a Nikon Eclipse NI-U model microscope (Tokyo, Japan) equipped with a Nikon DS-Ri2 image capture system and loaded into Nikon’s NIS-Elements Basic Research software program for image analysis.

Wisnewski A.V, & Liu J. (2015). Immunochemical detection of the occupational allergen, methylene diphenyl diisocyanate (MDI), in situ. Journal of immunological methods, 429, 60-65.

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Microscopic Imaging of Apoptosis

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Microscopic imaging was by an Eclipse Ni-U Model microscope (Nikon, Tokyo, Japan). Phase-contrast cellular morphology and Hoechst 33,258 fluorescence nuclear morphology were performed as previously described.27 (link) Nuclei that were brightly stained, granular, and punctate were considered apoptotic.

Yu S., Wu N., Zhu J., Liu Y, & Han J. (2020). Pyrrolidine Dithiocarbamate Facilitates Arsenic Trioxide Against Pancreatic Cancer via Perturbing Ubiquitin-Proteasome Pathway. Cancer Management and Research, 12, 13149-13159.

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