Single-cell suspensions from rectal pinches were prepared as previously described30 (link). Rectal intraepithelial lymphocytes (IEL) and lamina propria(LP) were collected and subjected to flow cytometry analysis. The single-cell suspensions were first incubated with Fc Receptor blocking reagent (Miltenyi Biotec), followed by staining with viability dye (Invitrogen). The antibody mixtures were then incubated as previously described27 (link). For immune activation, the following antibodies were used: CD45-PerCP, CD3-PE-Cy7, CD4-BV605, CD8-APC-Cy7, CD14-V450, Ki67-APC, HLA-DR PE-Cy5, and CCR5-PE (BD Pharmingen); CD69-Alexa Fluor 700 (Biolegend); and CD38-FITC (STEMCELL Technologies). For detection of Treg and MDSCs, the following antibody mixture were used: CD45-PerCP/Cy5.5, CD3-PE-Cy7, CD4-BV605, CD8-BV785, lin 1-FITC (BD Pharmingen), FOXP3-APC (eBioscience), HLA-DR-APC-Cy7, CD11b-PE-Cy5, CD14-BV711, CD8-BV785, CD25-BV421, CD15-Alexa700 (Biolegend), CD33-PE (Milteny). An LSRII flow cytometer was used for data acquisition. FlowJo software (Tree Star Inc.) was used for data analyses.
Cd15 alexa700
Manufactured by BioLegend
CD15-Alexa700 is a fluorescently labeled antibody that binds to the CD15 antigen, also known as Lewis X or stage-specific embryonic antigen-1 (SSEA-1). It is a cell-surface carbohydrate determinant expressed on granulocytes, monocytes, and some stem cells. This product is designed for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Foxp3 apc, by Thermo Fisher Scientific (2 mentions)
Cd3 pe cy7, by BD (2 mentions)
Cd45 percp, by BD (2 mentions)
Cd8 apc cy7, by BD (2 mentions)
Hla dr apc cy7, by BioLegend (2 mentions)
Cd4 bv605, by BD (2 mentions)
Cd14 v450, by BD (2 mentions)
Cd38 fitc, by STEMCELL (2 mentions)
Viability dye, by Thermo Fisher Scientific (2 mentions)
Ki67 apc, by BD (2 mentions)
Hla dr pe cy5, by BD (2 mentions)
Ccr5 pe, by BD (2 mentions)
Cd69 alexa fluor 700, by BioLegend (2 mentions)
Cd8 bv785, by BD (2 mentions)
Cd11b pe cy5, by BioLegend (2 mentions)
Cd14 bv711, by BioLegend (2 mentions)
Cd8 bv785, by BioLegend (2 mentions)
Cd25 bv421, by BioLegend (2 mentions)
Cd45 percp cy5, by BD (2 mentions)
Lin1 fitc, by BD (2 mentions)
2 protocols using cd15 alexa700
1
Rectal Immune Cell Characterization
2
Rectal Immune Cell Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Single-cell suspensions from rectal pinches were prepared as previously described30 (link). Rectal intraepithelial lymphocytes (IEL) and lamina propria(LP) were collected and subjected to flow cytometry analysis. The single-cell suspensions were first incubated with Fc Receptor blocking reagent (Miltenyi Biotec), followed by staining with viability dye (Invitrogen). The antibody mixtures were then incubated as previously described27 (link). For immune activation, the following antibodies were used: CD45-PerCP, CD3-PE-Cy7, CD4-BV605, CD8-APC-Cy7, CD14-V450, Ki67-APC, HLA-DR PE-Cy5, and CCR5-PE (BD Pharmingen); CD69-Alexa Fluor 700 (Biolegend); and CD38-FITC (STEMCELL Technologies). For detection of Treg and MDSCs, the following antibody mixture were used: CD45-PerCP/Cy5.5, CD3-PE-Cy7, CD4-BV605, CD8-BV785, lin 1-FITC (BD Pharmingen), FOXP3-APC (eBioscience), HLA-DR-APC-Cy7, CD11b-PE-Cy5, CD14-BV711, CD8-BV785, CD25-BV421, CD15-Alexa700 (Biolegend), CD33-PE (Milteny). An LSRII flow cytometer was used for data acquisition. FlowJo software (Tree Star Inc.) was used for data analyses.
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