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Fitc conjugated ct b

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FITC-conjugated CT-B is a fluorescently labeled version of the cholera toxin subunit B, which is commonly used as a retrograde neuronal tracer. The FITC (fluorescein isothiocyanate) dye is conjugated to the CT-B protein, allowing for visualization of neuronal pathways and connections.

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Lab products found in correlation

Mouse anti flag m2, by Merck Group (1 mentions) Anti fam21, by Merck Group (1 mentions) Anti c myc, by BD (1 mentions)

2 protocols using fitc conjugated ct b

1

Antibody Panel for Cell Biology

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The following antibodies were used in this study: anti-FLAG (M2, mouse, Sigma-Aldrich), anti-HA (F-7, mouse, Santa Cruz Biotechnology), anti-HA (Y-11, rabbit, Santa Cruz Biotechnology), anti-CAV1 (D46G3, rabbit, Cell Signaling), anti-CAV1 (2297, mouse, BD Biosciences), anti-strumpellin (C-14, rabbit, Santa Cruz Biotechnology), anti-FAM21 (polyclonal, rabbit, EMD Millipore), anti-CCDC53 (polyclonal, rabbit, EMD Millipore), anti-WASH1 (polyclonal, rabbit, EMD Millipore), anti-SWIP (polyclonal, rabbit, EMD Millipore), anti-GAPDH (6C5, mouse, Santa Cruz Biotechnology), Alexa 555-conjugated phalloidin A (Molecular Probes), anti-GM130 (35, mouse, BD Biosciences), anti-c-myc (polyclonal, goat, Bethyl), anti-α5 integrin (CD49e, mouse, BD Biosciences), anti-α5 integrin (C-9, mouse, Santa Cruz Biotechnology), anti-LAMP1 (H4A3, mouse, abcam), anti-α-tubulin (1E4C11, mouse, Proteintech), anti-β-tubulin (D66, mouse, Sigma-Aldrich), anti-SNX1 (51, mouse, BD Biosciences), FITC-conjugated CT-B (Molecular Probes), and anti-GFP (598, rabbit, MBL). The working dilutions for each antibody are listed in table S2.
Lee S., Park H., Zhu P.P., Jung S.Y., Blackstone C, & Chang J. (2020). Hereditary spastic paraplegia SPG8 mutations impair CAV1-dependent, integrin-mediated cell adhesion. Science signaling, 13(613), eaau7500.
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2

CT-B Internalization in hTERT-RPE1 Cells

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hTERT-RPE1 cells grown on cover slips were washed with ice-cold culture media, then incubated with FITC-conjugated CT-B (Molecular Probes) at 4 °C for 30 min. After washing with warm culture media, cells were placed at 37 °C for 1 h to permit CT-B internalization (56 (link)). Cells were then fixed with 4% paraformaldehyde and immunostained.
Lee S., Park H., Zhu P.P., Jung S.Y., Blackstone C, & Chang J. (2020). Hereditary spastic paraplegia SPG8 mutations impair CAV1-dependent, integrin-mediated cell adhesion. Science signaling, 13(613), eaau7500.
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