APOE in human plasma was measured using the Human Apolipoprotein E Human ELISA Kit (Abcam, ab108813). ApoE in mouse macrophage media was measured with Mouse Apolipoprotein E SimpleStep ELISA Kit (Abcam, ab215086). Human CXCL1 was measured with Human CXCL1/GRO alpha Quantikine ELISA Kit (R&D Systems, DGR00B). Mouse bone marrow cells were plated in 1:1 tumor cell conditioned media (CM):DMEM with 10% FBS for seven days before media were removed. For APOE detection, human plasma and macrophage CM were diluted 1:1,000 and 1:100, respectively, and measured in duplicate. APOE or CXCL1 levels in PDAC patients were stratified by APOE or CXCL1 levels (top and bottom quartile). Survival analysis was performed using log-rank test.
Mouse apolipoprotein e simplestep elisa kit
Manufactured by Abcam
Sourced in United Kingdom
The Mouse Apolipoprotein E SimpleStep ELISA Kit is a quantitative assay designed to measure mouse apolipoprotein E levels in biological samples. It utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique.
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2 protocols using mouse apolipoprotein e simplestep elisa kit
1
Quantifying APOE and CXCL1 in PDAC
2
Measuring Murine Apolipoprotein E Levels
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After intraperitoneal injection of a 100-μL pentobarbital sodium (70 mg/kg)-xylazine (12 mg/kg) anesthesia mixture, mice with loss of righting reflex were immediately killed by exsanguination. Then, the bronchus was incised, and bronchoalveolar lavage fluid (BALF) was collected with 1 mL of saline.
Mouse Apolipoprotein E Simple Step ELISA® Kit (ab215086; Abcam PLC, Cambridge, UK) was used for assay according to the manufacturer’s protocol. The BALF sample was diluted and used as a sample for measurement. All reagents were used after being brought to room temperature. The diluted sample or standard solution and the specific antibody solution were added to each well and shaken at room temperature for 1 h. Each well was washed with wash buffer. Then, a light-emission detection reagent was added, and the solutions were shaken for 10 min in a light-shielding condition. Finally, reaction stop solution was added to each well, and after shaking for 1 h, the absorbance at 450 nm was measured using an Envision system (Perkin Elmer Japan G.K., Kanagawa, Japan), and the apolipoprotein E (ApoE) concentration was calculated.
Mouse Apolipoprotein E Simple Step ELISA® Kit (ab215086; Abcam PLC, Cambridge, UK) was used for assay according to the manufacturer’s protocol. The BALF sample was diluted and used as a sample for measurement. All reagents were used after being brought to room temperature. The diluted sample or standard solution and the specific antibody solution were added to each well and shaken at room temperature for 1 h. Each well was washed with wash buffer. Then, a light-emission detection reagent was added, and the solutions were shaken for 10 min in a light-shielding condition. Finally, reaction stop solution was added to each well, and after shaking for 1 h, the absorbance at 450 nm was measured using an Envision system (Perkin Elmer Japan G.K., Kanagawa, Japan), and the apolipoprotein E (ApoE) concentration was calculated.
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