Anti g3bp1

Manufactured by BD

Sourced in United States

Anti-G3BP1 is a laboratory reagent used in research applications. It is an antibody that specifically targets the G3BP1 protein, which is involved in the regulation of cellular processes. The core function of Anti-G3BP1 is to facilitate the detection and analysis of the G3BP1 protein in various experimental settings.

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Lab products found in correlation

Anti phospho eif2α, by Cell Signaling Technology (3 mentions) Anti p erk, by Cell Signaling Technology (2 mentions) Anti eif2α, by Cell Signaling Technology (2 mentions) Nupage lds sample buffer, by Thermo Fisher Scientific (1 mentions) Odyssey blocking buffer, by LI COR (1 mentions) Phosstop, by Roche (1 mentions) Iblot 2 transfer device, by Thermo Fisher Scientific (1 mentions) Anti mcherry, by Abcam (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions) Proteinase inhibitor cocktail, by Roche (1 mentions) Odyssey fc imaging system, by LI COR (1 mentions) Anti eif2α, by Santa Cruz Biotechnology (1 mentions) Irdye 800cw 680rd, by LI COR (1 mentions) Anti β actin, by Merck Group (1 mentions) Cycloheximide (chx), by Fujifilm (1 mentions) Anti puromycin, by Merck Group (1 mentions) Thapsigargin, by Fujifilm (1 mentions) Gcn2ib, by MedChemExpress (1 mentions) Anti phospho gcn2, by Abcam (1 mentions)

Spelling variants of this product

G3BP1 (10 citations) Mouse anti-G3BP1 (7 citations) Anti-G3BP1 antibody (2 citations)

5 protocols using anti g3bp1

Cell Lysis and Western Blot Analysis

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Cells were collected using PBS and lysed for 10 min on ice using RIPA buffer (25 mM Tris-HCl (pH 7.6), 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS; Pierce, 89901) supplemented with proteinase inhibitor cocktail (Roche 1186153001) and PhosSTOP (Roche 04906845001). Samples were centrifuged for 20 min at 4°C at 14,000 rpm. 4X NuPAGE LDS sample buffer (Thermo Fisher Scientific NP0008) was added to the supernatant and samples were boiled for 5 min. Samples were run in 4–12% NuPAGE Bis-Tris gels (Invitrogen) and transferred to nitrocellulose membranes using an iBlot 2 transfer device (Thermo Fisher Scientific). Membranes were blocked with Odyssey blocking buffer (LI-COR) and then incubated with primary antibodies. Following incubation with dye-labeled secondary antibodies, signals were visualized using an Odyssey Fc imaging system (LI-COR). Primary western blot antibodies were anti-β-actin (Santa Cruz Biotechnology sc-1616), anti-eIF2α (Santa Cruz Biotechnology sc-133132), anti-phospho-eIF2α (Cell Signaling 3597S), anti-mCherry (Abcam 167453), and anti-G3BP1 (BD biosciences 6111126). Secondary western blot antibodies were IRDye 800CW/680RD (LI-COR) used at a dilution of 1:15,000.

Zhang P., Fan B., Yang P., Temirov J., Messing J., Kim H.J, & Taylor J.P. (2019). Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology. eLife, 8, e39578.

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Cellular stress response regulation assay

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24S-OHC [52 (link)] was dissolved in EtOH (Wako, Osaka, Japan). F12511 was the generous gift of Kowa (Aichi, Japan). Thapsigargin and CHX were purchased from Wako (Osaka, Japan). GSK2606414 and ISRIB were from Cayman Chemical (Ann Arbor, MI, USA). GCN2iB was from MedChemExpress (Monmouth Junction, NJ, USA). Thapsigargin, GSK2606414, CHX, and ISRIB were dissolved in dimethyl sulfoxide (DMSO; Wako). The following antibodies were from commercial sources: anti-PERK (Cat# 3192), anti-phospho-eIF2α (Cat# 3398), anti-eIF2α (Cat# 5324), and anti-GCN2 (Cat# 3302) were from Cell Signaling (Danvers, MA, USA); anti-β-actin (Cat# A5441) was from Sigma-Aldrich (St. Louis, MO, USA); anti-TIA1 (Cat# 12133-2-AP), anti-ATF4 (Cat# 10835-1-AP), and anti-TDP-43 (Cat# 12782-2-AP) were all from Proteintech (Chicago, IL, USA); anti-G3BP1 (Cat# 611126) was from BD Biosciences (Franklin Lakes, NJ, USA); anti-phospho-GCN2 (Cat# ab75836) was from Abcam (Cambridge, UK); and anti-puromycin (Cat# MABE343) was from Merck Millipore (Burlington, MA, USA); All other chemicals, of analytical grade, were obtained from Sigma-Aldrich or Wako.

Urano Y., Osaki S., Chiba R, & Noguchi N. (2022). Integrated stress response is involved in the 24(S)-hydroxycholesterol-induced unconventional cell death mechanism. Cell Death Discovery, 8, 406.

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Antibody Characterization for bRSV Proteins

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Mouse monoclonal antibodies raised against bRSV N (mAb89), P (mAb12), and M2-1 (mAb90) were previously described (52 (link), 53 (link)). Rabbit monoclonal, anti-RPL3, and anti-RPL18 were purchased from Affinity Biosciences. Rabbit anti-RPS6 (2217), anti-eIF4G (2467), anti-eIF4E (2067), anti-eIF3A (3411), anti-eIF4A (2013), anti-eIF4A1 (2490), anti-eIF4B (3592), anti-eIF4H (3469), and anti-GAPDH (5174) were obtained from Cell Signalling Technology (CST). Mouse anti-PABP (ab6125) was obtained from Abcam, anti-G3BP-1 was from BD Biosciences, rat anti-GFP was from BioLegend, and Rabbit anti-RPS16 (HPA064222), mouse anti-puromycin, and sodium arsenite were purchased from Sigma, Merck. Secondary horseradish peroxidase-linked antibodies were obtained from CST and Alexa Fluor secondary antibodies were from Life Technologies.

Jobe F., Kelly J.T., Simpson J., Wells J., Armstrong S.D., Spick M., Lacey E., Logan L., Geifman N., Hawes P, & Bailey D. (2024). Viral PIC-pocketing: RSV sequestration of translational preinitiation complexes into bi-phasic biomolecular condensates. Journal of Virology, 98(3), e00153-24.

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Antibody Reagents for Protein Analysis

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Anti-α-tubulin monoclonal antibody was purchased from Sigma-Aldrich. Anti-eIF4γ, anti-BiP/Grp78, anti-PKCδ and anti-G3BP1 monoclonal antibodies were from BD Bioscience; anti-Atg5, anti-Atg16L1, anti-eIF2α, anti-phospho-eIF2α, anti-cleaved caspase7 (cCas7), anti-PERK, anti-cleaved poly(ADP-ribose) polymerase (cPARP), anti-PKD1, anti-phospho-(Ser/Thr) PKD substrates and anti-TIAR antibodies were from Cell Signaling Technology; anti-DAP1, phospho-PKD1 (S738/742) and phospho-PKD1 (S916) antibodies were from Abcam; anti-ATF4 antibody was from Santa Cruz Biotechnology; and anti-GAPDH, anti-PKD2 and anti-PKD3 antibodies were from GeneTex. Anti-DnaK antibody was obtained from ENZO. Anti-SubAB antibody was prepared as previously described (Yahiro et al., 2006 (link)). PKC inhibitor Gö6976 was obtained from LC Laboratories; PKC activator PMA, PKC inhibitor Gö6983, PKD/PKCμ inhibitor CID755673, PKA activator 8Br-cAMP, Thapsigargin (TG), CaM kinase II inhibitor KN-93 were from Sigma Aldrich; and PKA inhibitor 14–22 Amide was from Calbiochem; PKC inhibitor Bisindolylmaleimide II was from ALEXIS Biochemicals; and ROCK II inhibitor Y-27632 was from Cayman Chemical.

Tsutsuki H., Yahiro K., Ogura K., Ichimura K., Iyoda S., Ohnishi M., Nagasawa S., Seto K., Moss J, & Noda M. (2016). Subtilase cytotoxin produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli induces stress granule formation. Cellular microbiology, 18(7), 1024-1040.

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Immunochemical Procedures for Protein Detection

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For immunochemical procedures, the following primary antibodies were used: mouse anti-ataxin-2 (1:1000, ref. 611378, BD Biosciences); mouse anti-ubiquitin (1:1000, ref. 3936S, Cell Signaling) rabbit anti-DARPP-32 (1:1000, ref. AB10518, Merck Millipore); rabbit anti-G3BP1 (1:1000, ref. 07-1801, Millipore); mouse anti-human G3BP1 (1:1000, ref. 611126, BD Biosciences); anti-G3BP1 (1:1000, ref. 05-1938; Sigma-Aldrich); mouse anti-GFAP (1:1000, ref. 644702, BioLegend); rabbit anti-HA (1:1000, ref. Ab9110, Abcam); mouse anti-calbindin D-28K (1:1000, ref. C9848, Sigma-Aldrich); mouse anti-PABP-1 (1:1000, ref. 04-1467, Millipore) and mouse β-Gal (14B7) (1:500, ref. 2372, Cell Signaling Technology). For fluorescence immune procedures, the following antibodies were used: anti-mouse AlexaFluor 647 (1:200, ref. A21235, Invitrogen), anti-mouse AlexaFluor 488 (1:200, ref. A11001, Invitrogen), anti-rabbit AlexaFluor 488 (1:200, ref. A11008, Invitrogen), anti-mouse AlexaFluor 594 (1:200, ref. A11005, Invitrogen) and anti-rabbit AlexaFluor 594 (1:200, ref. A11012, Invitrogen). For light imaging, the following antibodies were used: anti-mouse biotinylated (1:200, ref. BA-9200, Vector Laboratories) and anti-rabbit biotinylated (1:200, ref. BA-1000, Vector Laboratories).

Koppenol R., Conceição A., Afonso I.T., Afonso-Reis R., Costa R.G., Tomé S., Teixeira D., da Silva J.P., Côdesso J.M., Brito D.V., Mendonça L., Marcelo A., Pereira de Almeida L., Matos C.A, & Nóbrega C. (2022). The stress granule protein G3BP1 alleviates spinocerebellar ataxia-associated deficits. Brain, 146(6), 2346-2363.

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