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Human whole blood

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Human whole blood is a biological product consisting of various cellular components, including red blood cells, white blood cells, and platelets, suspended in plasma. It is a complex and heterogeneous sample that can be used for a variety of research and analytical applications.

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Lab products found in correlation

Streptozotocin (stz), by MedChemExpress (2 mentions) Matched paired insulin antibodies, by BBI Solutions (2 mentions) Bm364 z8a2, by BBI Solutions (2 mentions) Bm364 t8f5, by BBI Solutions (2 mentions) Human insulin, by R&D Systems (2 mentions) 15 μm superavidin coated microspheres, by Bangs Laboratories (2 mentions) Humulin r and humulin h, by Eli Lilly (2 mentions) R pe antibody conjugation kit, by Thermo Fisher Scientific (2 mentions) Glucose aptamer and dna competitor oligonucleotides, by Integrated DNA Technologies (2 mentions)

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Whole blood (2 citations)

4 protocols using human whole blood

1

Recombinant SARS-CoV-2 Spike Protein Assay

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The magnetic beads (Dynabeads® MyOne™ Carboxylic Acid, 65011) were bought from Life Technologies Corporation (Carlsbad, CA). 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, E1769-5G), N-hydroxy succinimide (NHS, 130672-5G), negative control human serum (H4522-20 ML), and single-stranded salmon sperm DNA (ssDNA, D7656-1 ML) were bought from Sigma (St. Louis, MO). Human whole blood was bought from BioIVT (Westbury, NY). Recombinant SARS-CoV-2 spike subunit 1 (S1) protein (40591-V08H, 40591-V08B1) and chimeric anti-SARS-CoV-2 IgG (40150-D006) were bought from Sino Biological (Chesterbrook, PA). Recombinant SARS-CoV-2 S1 protein (RP01261) was from ABclonal Technology (Woburn, MA). Recombinant SARS-CoV-2 S1 protein (PNA002) and receptor-binding domain (RBD) protein (PNA004) were bought from Sanyou Biopharmaceuticals Co., Ltd (Shanghai, China). Another recombinant RBD protein (NR-52306) was provided by BEI Resources. The goat anti-human IgG (AffiniPure Goat Anti-Human IgG, 109-005-170) was bought from Jackson ImmunoResearch Laboratories, Inc (Pennsylvania, USA).
Zhang P., Chen L., Hu J., Trick A.Y., Chen F.E., Hsieh K., Zhao Y., Coleman B., Kruczynski K., Pisanic TR I.I., Heaney C.D., Clarke W.A, & Wang T.H. (2021). Magnetofluidic immuno-PCR for point-of-care COVID-19 serological testing. Biosensors & Bioelectronics, 195, 113656.
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2

Insulin Detection in Human Blood

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All chemicals were purchased from Sigma-Aldrich, unless otherwise noted. 15-μm SuperAvidin-coated microspheres were obtained from Bangs Laboratories, human whole blood was obtained from BioIVT, matched paired insulin antibodies (capture: BM364-Z8A2, detection: BM364-T8F5) were obtained from BBI Solutions, human insulin was obtained from R&D Systems, Streptozotocin (STZ) was obtained from MedChem Express, Humulin R and Humulin H were obtained from Eli Lilly, the R-PE antibody conjugation kit was obtained from Thermo Fisher Scientific, and the glucose aptamer and DNA competitor oligonucleotides (see Table 1 for sequences) were ordered from Integrated DNA Technologies.
Poudineh M., Maikawa C.L., Ma E.Y., Pan J., Mamerow D., Hang Y., Baker S.W., Beirami A., Yoshikawa A., Eisenstein M., Kim S., Vučković J., Appel E.A, & Soh H.T. (2020). A fluorescence sandwich immunoassay for the real-time continuous detection of glucose and insulin in live animals. Nature biomedical engineering, 5(1), 53-63.
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3

Insulin Detection in Human Blood

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All chemicals were purchased from Sigma-Aldrich, unless otherwise noted. 15-μm SuperAvidin-coated microspheres were obtained from Bangs Laboratories, human whole blood was obtained from BioIVT, matched paired insulin antibodies (capture: BM364-Z8A2, detection: BM364-T8F5) were obtained from BBI Solutions, human insulin was obtained from R&D Systems, Streptozotocin (STZ) was obtained from MedChem Express, Humulin R and Humulin H were obtained from Eli Lilly, the R-PE antibody conjugation kit was obtained from Thermo Fisher Scientific, and the glucose aptamer and DNA competitor oligonucleotides (see Table 1 for sequences) were ordered from Integrated DNA Technologies.
Poudineh M., Maikawa C.L., Ma E.Y., Pan J., Mamerow D., Hang Y., Baker S.W., Beirami A., Yoshikawa A., Eisenstein M., Kim S., Vučković J., Appel E.A, & Soh H.T. (2020). A fluorescence sandwich immunoassay for the real-time continuous detection of glucose and insulin in live animals. Nature biomedical engineering, 5(1), 53-63.
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4

MasSpec Pen Device and Priming

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Chemicals and Reagents. Sterile water (Deerfield, Illinois, USA) commonly used during surgery was provided by the clinical staff and used for all analyses. Human whole blood was purchased from BioIVT (Westbury, NY, USA).
Silicon tubing, PTFE tubing, and heat shrink tubing were purchased from Kinesis (Vernon Hills, IL, USA), MilliporeSigma (Burlington, MA, USA), and Newark element14 (Chicago, IL, USA). Luer-locks were purchased from Cole-Parmer (Vernon Hills, Illinois, USA). The other materials used to 3D-print the MasSpec Pen devices were described in detail in our previous report.( 28)
MasSpec Pen Device and Operation. The MasSpec Pen devices were produced in the laboratory as previously described (28) . All devices were discarded after each surgery observing universal precaution measures. of the MasSpec Pen devices. The control box was equipped with a switch to alternate the "priming" and "analysis" functions between the two syringe pumps. Under "priming" function mode, the syringe pump was activated by pressing the "Refill" button, allowing pumping of 2.5 mL of water to fill the 4.5 meter PTFE tube (~ 15 seconds).
Zhang J., Sans M., DeHoog R.J., Garza K.Y., King M.E., Feider C.L., Bensussan A., Keating M.F., Lin J.Q., Povilaitis S.C., Katta N., Milner T.E., Yu W., Nagi C., Dhingra S., Pirko C., Brahmbhatt K.A., Buren G.V., Carter S., Fisher W.E., Thompson A.M., Grogan R.H., Suliburk J., & Eberlin L.S. (2020). Direct Molecular Analysis of In Vivo and Freshly Excised Tissues in Human Surgeries with the MasSpec Pen Technology.
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