The A549 and H1299 human lung cancer cells were provided by the Pharmacology Laboratory of Harbin Medical University, and the most recent STR identification was in September 2023 (Figure S1A,B ). BALB/c Nude mice were purchased from Beijing Viton Lihua Laboratory Animal Technology Co. The experimental compound EEBR was provided by the Laboratory of Natural Drugs and Medicinal Chemistry, Harbin Medical University, while cisplatin was obtained from Qilu Pharmaceutical Co., LTD. VX‐765 and BAY 11‐7082 inhibitors were purchased from Dalian Meilun Biotechnology Co., LTD. Trypsin, 1640 medium and penicillin/streptomycin were supplied by Gibco (Carlsbad, CA, USA), while fetal bovine serum (FBS) was obtained from Clark Corporation, USA. The Cell Counting Kit‐8 (CCK8) was purchased from Dojindo and the BCA protein quantitation kit from Beyotime. The antibodies specific for NF‐κB (cat# A10609), NLRP3 (cat# A5652), cleaved Caspase‐1 (cat# A23429) and GSDMD (cat# A20728) were purchased from ABclonal; N‐terminal GSDMD (cat# ab215203) was purchased from Abcam; β‐actin (cat# bs‐0061R), Caspase‐1 (cat# bs‐10442R), interleukin‐18 (IL‐18) (cat# bs‐4986R), interleukin‐1 beta (IL‐1β) (cat# bs‐25615R) antibodies were obtained from Bioss.
Cleaved caspase 1
Manufactured by ABclonal
Sourced in China
Cleaved Caspase‐1 is a lab equipment product manufactured by ABclonal. It is a protein involved in the activation of the inflammatory response and programmed cell death (apoptosis). The core function of Cleaved Caspase‐1 is to serve as a biochemical marker for these cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Il 1β, by ABclonal (2 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
1640 medium, by Thermo Fisher Scientific (1 mentions)
Caspase 1, by Bioss Antibodies (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
β actin, by Abcam (1 mentions)
Bca protein quantitation kit, by Beyotime (1 mentions)
Cisplatin, by Qilu Pharmaceutical (1 mentions)
Vx765, by Meilun (1 mentions)
Gsdmd, by ABclonal (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Solarbio (1 mentions)
Histone h3, by GeneTex (1 mentions)
Bca protein assay kit, by Solarbio (1 mentions)
Gapdh, by Proteintech (1 mentions)
Myd88, by ABclonal (1 mentions)
Nlrp3, by ABclonal (1 mentions)
Gsdmd n, by Affinity Biosciences (1 mentions)
Tunel detection kit, by Beyotime (1 mentions)
Ml385, by MedChemExpress (1 mentions)
3 protocols using cleaved caspase 1
1
Investigating EEBR's Anti-Cancer Effects
2
Western Blot Analysis of NLRP3 Inflammasome
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Total protein was extracted from nasal mucosa using RIPA lysis buffer and PMSF. Protein concentration was determined using the Bicinchoninic acid (BCA) Protein Assay kit (Solarbio, Beijing, China). Equal amount of protein (10–20 µg) from each sample was isolated using SDS-PAGE (8%, 12%, and 15% gel) and transferred to the polyvinylidene difluoride (PVDF) membranes. After blocking with 5% nonfat milk for 1 h at room temperature, the membranes were incubated with primary antibodies at 4°C overnight, followed by the incubation of horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3,000, Solarbio) for 1 h at 37°C. Subsequently, the membranes were visualized with electrochemiluminescence (Solarbio) for luminescence generation. GAPDH served as inner control for normalization. The primary antibodies used in present study were: the nucleotide binding and oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3, 1:1,000, ABclonal, Wuhan, China), apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC, 1:1,000, ABclonal), cleaved caspase-1 (1:500, ABclonal), IL-1β (1:1,000, ABclonal), TLR4 (1:1,000, ABclonal), MyD88 (1:1,000, ABclonal), p65/p-p65 (Ser536) (1:1,000, Affinity, Cincinnati, OH, USA), GAPDH (1:10,000, Proteintech, Wuhan, China), and Histone H3 (1:1,000, GeneTex, San Antonio, TX, USA).
3
Airborne Particulate Matter Analysis
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PM (The standard reference airborne 1649b) was purchased from the National Institute of Standards and Technology (NIST, Gaithersburg, USA). PAL (powder) was obtained from Macklin (Shanghai, China). TUNEL detection kit and ROS detection kit were purchased from Beyotime (Shanghai, China). The ELISA kits of IL-18, IL-6, IL-8, SOD, MDA, and IL-1β were purchased from Boyun Biotechnology (Shanghai, China). The DMEM/F12 medium, PBS, and penicillin-streptomycin were purchased from Gibco (USA), and the fetal bovine serum (FBS) was purchased from Sijiqing (Hangzhou, China). RIPA lysis buffer and phenylmethylsulfonyl fluoride (PMSF) were purchased from Solarbio (Beijing, China) while the phosphatase inhibitors were purchased from Applygen (Beijing, China). Antibodies against Nrf2, ASC, IL-18, IL-1β, Cleaved-caspase-1, and β-actin were purchased from Abclonal (Wuhan, China); antibodies against NLRP3, HO-1, NQO1, and N-GSDMD were purchased from Affinity (Jiangsu, China); antibody against EpCAM was purchased from Santa (Cruz, USA); the secondary antibody for Western Blotting was purchased from Biosharp (Beijing, China); ML385 was purchased from MedChemExpress (Shanghai, China).
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