Streptavidin hrp vectastain abc kit

Frozen tissue samples were fixed in 3.7% formaldehyde (FA) overnight at 4°C, transferred into 1% FA until they were embedded in paraffin. Paraffin samples were cut into 5 µm sections and stained with hematoxylin and eosin. Tumor grades in histological sections were classified into aberrant crypt foci (ACF), adenoma (Ad), and mucosal carcinoma. Detection of IL-23 in the tumor tissue was performed with an anti-IL-23p19 antibody (rabbit polyclonal, Abcam). Proliferating cells were detected via Ki-67 staining with a rat monoclonal antibody (Dako). Secondary anti-rabbit and anti-rat (Vector Laboratories, Inc.) antibodies coupled to biotin were added and sections were developed via the streptavidin–HRP VECTASTAIN^® ABC Kit (Vector Laboratories, Inc.). Finally, color development was performed with 3-amino-9-ethylcarbazole (AEC; Dako). All sections were counterstained with Harris hematoxylin solution (Sigma Aldrich).

Per and PB were purchased from Sigma-Aldrich. Primary antibodies anti-interleukin-1β (IL-1β), anti-brain derived neurotrophic factor (BDNF) were purchased from Santacruz Biotechnology (Dallas, TX, USA). Species specific biotinylated secondary antibodies and streptavidin-HRP (Vectastain ABC Kit) were purchased from Vector laboratories (Burlingame, CA, USA). All other chemicals used in the present study were purchased from Sigma unless specified. Animal tissues were sent for paraffin embedding and sectioning to AML Laboratories (St. Augustine, FL, USA). Fecal samples from experimental mice groups and GW Veterans were sent to COSMOSID (Germantown, MD, USA) for whole-genome sequencing.