Pp h6705 00

Manufactured by R&D Systems

The PP-H6705-00 is a compact and versatile laboratory instrument designed for precise liquid handling tasks. It features a high-performance, reliable liquid dispensing system that can accurately deliver volumes ranging from microliters to milliliters. The device is suitable for a variety of laboratory applications that require precise and consistent liquid transfers.

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Lab products found in correlation

Hematoxylin, by Thermo Fisher Scientific (1 mentions) Pt link, by Agilent Technologies (1 mentions) Dab chromagen, by Agilent Technologies (1 mentions) Harris modified hematoxylin, by Thermo Fisher Scientific (1 mentions) Vectastain abc reagent, by Agilent Technologies (1 mentions) Vectastain kit, by Vector Laboratories (1 mentions) Ab7260, by Abcam (1 mentions) Vinculin, by Cell Signaling Technology (1 mentions) Na934v, by GE Healthcare (1 mentions) Nxa931, by GE Healthcare (1 mentions) Phospho atm ser1981, by Cell Signaling Technology (1 mentions) Enhanced chemi luminescence (ecl), by Thomas Scientific (1 mentions) β actin, by Merck Group (1 mentions)

3 protocols using pp h6705 00

Immunohistochemical staining for ERRβ in breast cancer

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IHC staining of breast cancer tissue was performed for ERRβ. Five micron sections from formalin fixed paraffin embedded tissues were de-paraffinized with xylenes and rehydrated through a graded alcohol series. Heat induced epitope retrieval (HIER) was performed by immersing the tissue sections in Target Retrieval Solution, Low pH (DAKO) in the PT Link (DAKO). IHC staining was performed using the VectaStain Kit from Vector Labs according to manufacturer’s instructions. Briefly, slides were treated with 3% hydrogen peroxide, avidin/biotin blocking, and 10% normal goat serum and independently exposed to primary antibodies for ERRβ2- cl .07, 1:150, 1:240 (R&D systems, #PP-H6707-00) and ERRβsf- cl .05, 1:150 (R&D systems, #PP-H6705-00) for 1 hour at room temperature. Slides were exposed to appropriate biotin-conjugated secondary antibodies (Vector Labs), Vectastain ABC reagent and DAB chromagen (Dako). Slides were counterstained with Hematoxylin (Fisher, Harris Modified Hematoxylin), blued in 1% ammonium hydroxide, dehydrated, and mounted with Acrymount. Control tissues with the primary antibody omitted were used as negative controls. Images of the full TMA slide stained for of Hematoxylin and eosin, ERRβsf- cl .05, and ERRβ2- cl .07 are available on figshare (https://doi.org/10.6084/m9.figshare.9992891.v1).

Fernandez A.I., Geng X., Chaldekas K., Harris B., Duttargi A., Berry V.L., Berry D.L., Mahajan A., Cavalli L.R., Győrffy B., Tan M, & Riggins R.B. (2019). The orphan nuclear receptor estrogen-related receptor beta (ERRβ) in triple-negative breast cancer. Breast cancer research and treatment, 179(3), 585-604.

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Immunocytochemistry of Pluripotent Stem Cells

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Cells were fixed, permeabilized, incubated, stained and imaged as
described previously^{56 (link)}.
Briefly, cells were washed twice with PBS and fixed with 4% PFA/PBS for 15 min
at room temperature. Samples were permeabilized with 0.2% Triton X-100/PBS for
10 min at room temperature, then blocked with 3% BSA/PBS for 30 min at room
temperature. Cells were incubated with primary antibody (OCT4 (Santa Cruz,
sc-5279, 1: 50); SOX2 (R&D Systems, AF2018, 1: 20); NANOG (Active Motif,
61419, 1: 200), ESRRB (R&D Systems, PP-H6705–00, 1: 100); SOX1
(R&D Systems, AF3369, 1: 20), NES (MILLIPORE, MAB353, 1: 50), GABA
(Invitrogen, PA5–32241, 1: 100), GFAP (Abcam, ab7260, 1: 1000)) overnight
at 4°C. Fluorescence-conjugated species-specific secondary antibodies
(Invitrogen) were used for visualization.

Zhu Z., Chen X., Guo A., Manzano T., Walsh P.J., Wills K.M., Halliburton R., Radko-Juettner S., Carter R.D., Partridge J.F., Green D.R., Zhang J, & Roberts C.W. (2023). Mitotic bookmarking by SWI/SNF subunits. Nature, 618(7963), 180-187.

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Immunoblotting of DNA Damage Response

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Following polyacrylamide gel electrophoresis and protein transfer, nitrocellulose membranes were blocked in 5% nonfat dry milk*** dissolved in Tris-Buffered Saline with Tween-20 (TBST), then probed overnight at 4°C with the following primary antibodies in TBST: ERRbeta #PP-H6707-00 (cl.07) 1:250 - 1:500 and #PP-H6705-00 (cl.05) 1:500 – 1:750 (R&D Systems, Minneapolis, MN); PARP #9542 1:1000, phospho Ser139 (γ) histone H2A. X #9718 1:1000, ***total histone H2A. X #2595 1:1000, phospho Ser1981 ATM #5883 1:500, phospho Ser428 ATR #2853 1:500, phospho Thr68 Chk2 #2197 1:500, phospho Ser345 Chk1 #2348 1:500, phospho Ser10 histone H3 #3377 1:1000, total histone H3 #9715 1:1000, phospho Thr180/Tyr182 p38 MAPK #9216 1:250, p38 #9212 1:500, vinculin #13901 1:1000 (Cell Signaling, Danvers, MA). All membranes were re-probed with β-actin (Sigma #A5316 1:5000 – 1:10,000) as a loading control for ≥1 h at room temperature or overnight at 4°C. Horseradish peroxidase enzyme-conjugated anti-mouse or anti-rabbit whole immunoglobulin (IgG) secondary antibodies (GE #NXA931 and #NA934V, respectively, Buckinghamshire, U.K.) were used at 1:5000 for ≥1 h at room temperature, followed by enhanced chemiluminescence (ECL, Denville Scientific, Holliston, MA) as in [27 (link)]. ***Membranes to be probed for total histone H2A. X were blocked in 5% horse serum in TBST rather than milk.

Heckler M.M., Zeleke T.Z., Divekar S.D., Fernandez A.I., Tiek D.M., Woodrick J., Farzanegan A., Roy R., Üren A., Mueller S.C, & Riggins R.B. (2016). Antimitotic activity of DY131 and the estrogen-related receptor beta 2 (ERRβ2) splice variant in breast cancer. Oncotarget, 7(30), 47201-47220.

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