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Biotinylated alkyne

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Biotinylated Alkyne is a small molecule that contains a biotin group and an alkyne functional group. It can be used for various bioconjugation applications.

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Lab products found in correlation

Udp glca, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by R&D Systems (1 mentions) Cy5 alkyne, by Merck Group (1 mentions) Streptavidin alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Udp gal, by Merck Group (1 mentions) St6gal1, by Bio-Techne (1 mentions) Bis tris gel, by Thermo Fisher Scientific (1 mentions) Gdp fucose, by Merck Group (1 mentions) Streptavidin hrp, by R&D Systems (1 mentions) Sep pak c18 spe cartridge, by Waters Corporation (1 mentions) Gdp azido fucose, by R&D Systems (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions) Trifluoroacetic acid, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Ammonium bicarbonate, by Merck Group (1 mentions) 1 butanol, by Merck Group (1 mentions) Hplc grade ethanol, by Merck Group (1 mentions) Pngase f, by New England Biolabs (1 mentions)

2 protocols using biotinylated alkyne

1

Glycan Modification and Detection

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GDP-N3-Fucose, CMP-N3-sialic acid, UDP-N3-GalNAc, UDP-N3-GlcNAc, Biotinylated Alkyne and 4’,6-diamidino-2-phenylindole (DAPI) were from R&D Systems/Bio-techne. Recombinant human B4GalT1Y285L, GALNT2, FUT7, GCNT1, B3GNT6, EXT1/2, ST6Gal1, C1GalT1 and HPSE, and recombinant B. thetaiotaomicron O-GlcNAcase (OGA), P. heparinus heparinase III (Hep III), P. multocida hyaluronan synthase (HAS) and S. agalactiae hyaluronan lyase were also from R&D System/Bio-techne. Streptavidin-Alexa Fuor 555 and streptavidin-Alexa Fluor 488 were from ThermoFisher Scientific. Cy5-Alkyne, UDP-Gal and UDP-GlcA and all other small chemicals were from Sigma Aldrich.
Wu Z.L., Person A.D., Anderson M., Burroughs B., Tatge T., Khatri K., Zou Y., Wang L., Geders T., Zaia J, & Sackstein R. (2017). Imaging specific cellular glycan structures using glycosyltransferases via click chemistry. Glycobiology, 28(2), 69-79.
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2

NA Glycosylation and Mass Spectrometry

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Ammonium bicarbonate, dithiothreitol (DTT), iodoacetamide (IAA), trifluoroacetic acid (TFA), sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO), iodomethane (CH3I), sodium borohydride (NaBH4), 2,5-dihydroxybenzoic acid (DHB), urea, cellulose (medium fibrous), trypsin, HPLC grade ethanol, 1-butanol, and GDP-fucose were from Sigma-Aldrich. Sep-Pak C18 SPE cartridge was from Waters. PNGase F was from New England Biolabs. The NuPAGE series of LDS sample buffer (4×), MOPS SDS running buffer (20×), SimplyBlue SafeStain and 4-12% Bis-Tris Gels were from Life Technologies. 10,000 MWCO centrifugal devices were obtained from Millipore. NA was expressed with N-terminal His tag in sf21 insect cells and purified using nickel-histidine affinity chromatography followed by gel filtration as previously described [11 (link)]. Recombinant FUT8, FUT11, GDP-azido-fucose, biotinylated alkyne, and streptavidin-HRP were from R&D Systems.
Wu Z.L., Zhou H., Ethen C.M, & Reinhold V. (2016). Core-6 Fucose and the Oligomerization of the 1918 Pandemic Influenza Viral Neuraminidase. Biochemical and biophysical research communications, 473(2), 524-529.
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