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Ultraview universal dab detection

Manufactured by Roche
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The UltraView Universal DAB detection is a lab equipment product that provides a detection solution for immunohistochemistry assays. It is designed to enable the visualization of target proteins in biological samples.

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Lab products found in correlation

Benchmark ultra, by Roche (1 mentions) Aperio at2, by Leica (1 mentions) Dp22 digital camera, by Olympus (1 mentions) Liquid dab substrate chromogen system, by Agilent Technologies (1 mentions) Idh1 r132h mutant protein clone h09, by Dianova (1 mentions) P53 clone do 7, by Agilent Technologies (1 mentions) Atrx hpa001906, by Merck Group (1 mentions) Benchmark xt autostainer, by Roche (1 mentions) Benchmark xt, by Roche (1 mentions) Her2 antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

UltraView Universal diaminobenzidine (DAB) detection kit UltraView Universal DAB detection system XT UltraView Universal DAB Detection Kit UltraView Universal DAB and alkaline phosphatase detection kits UltraView Universal DAB (diaminobenzidine tetrahydrochloride) Detection Kit UltraView Universal DAB Detection Kit UltraView Universal DAB UltraView DAB

4 protocols using ultraview universal dab detection

1

Evaluating PD Effects of AZ'6421 in PDX

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Immunohistochemistry was used to evaluate the PD effects induced by increasing doses of AZ’6421 in CTC174 patient-derived xenograft (PDX) model study when compared to untreated and AZD9833 (10 mg/kg) treated tumours. The PDX xenograft tumours were formalin-fixed paraffin-embedded and sectioned at 4 µm. ER expression in tumours was assessed by IHC validated assays using the antibody clone SP1 (Ventana, Tucson, AZ) and detected using the UltraView Universal DAB detection on the Benchmark Ultra (Ventana, Tucson, AZ). Whole digital slide images were obtained using virtual microscopy (Aperio AT2, Leica Biosystems).
Hayhow T.G., Williamson B., Lawson M., Cureton N., Braybrooke E.L., Campbell A., Carbajo R.J., Cheraghchi-Bashi A., Chiarparin E., Diène C.R., Fallan C., Fisher D.I., Goldberg F.W., Hopcroft L., Hopcroft P., Jackson A., Kettle J.G., Klinowska T., Künzel U., Lamont G., Lewis H.J., Maglennon G., Martin S., Gutierrez P.M., Morrow C.J., Nikolaou M., Nissink J.W., O’Shea P., Polanski R., Schade M., Scott J.S., Smith A., Weber J., Wilson J., Yang B, & Crafter C. (2024). Metabolism-driven in vitro/in vivo disconnect of an oral ERɑ VHL-PROTAC. Communications Biology, 7, 563.
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2

Quantifying Angiogenesis in Multiple Myeloma

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Bone biopsy sections were incubated with mouse anti-human CD138 clone B-A38 (ready to use, Ventana Medical Systems, Tucson, AZ, USA) and the reaction was revealed with polymeric ultraView Universal DAB detection (Ventana Medical Systems) or with rabbit anti-human CD34 (1:100, Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing, sections were incubated with a secondary antibody (rat anti-Immunoglobulin G horseradish peroxidase (HRP); Millipore, Burlington, MA, USA; 1:250) and the reaction was revealed with a solution of 3,3′-diaminobenzidine tetrahydrochloride (Liquid DAB Substrate Chromogen System, DAKO, Glostrup, Denmark). MVD was evaluated as the number of CD34+ vessels/mm2 in MM patients’ bone biopsies. Images of IHC analyses were captured by a DP22 digital camera (Olympus, Hamburg, Germany) and analyzed with the OLYMPUS Stream software, adjusting tone and contrast to ensure the best image quality.
Marchica V., Toscani D., Corcione A., Bolzoni M., Storti P., Vescovini R., Ferretti E., Dalla Palma B., Vicario E., Accardi F., Mancini C., Martella E., Ribatti D., Vacca A., Pistoia V, & Giuliani N. (2019). Bone Marrow CX3CL1/Fractalkine is a New Player of the Pro-Angiogenic Microenvironment in Multiple Myeloma Patients. Cancers, 11(3), 321.
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3

Immunohistochemistry and FISH Analysis

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Immunohistochemistry was performed on formalin-fixed, paraffin embedded tissue sections at the UCSF Immunohistochemistry Laboratory and the UCSF Neuropathology BTRC Biomarkers Laboratory. Primary antibodies used were as follows: histone H3-K27M mutant protein (ABE419, EMD Millipore, Billerica, MA, 1:500 dilution), IDH1-R132H mutant protein (clone H09, DiaNova, Germany, 1:500 dilution), ATRX (HPA001906, Sigma, St Louis, MO, 1:100 dilution), p53 (clone DO-7, Dako, Glostrup, Denmark, 1:00 dilution), BRAF-V600E mutant protein (clone VE1, Ventana, Tucson, AZ). All staining was performed in Ventana or Leica Bond automated staining processors. Specifically, the histone H3-K27M immunostaining was run in a Ventana Benchmark XT autostainer using CC1 antigen retrieval buffer for 30 minutes at 95°C, incubation with 1:500 dilution of primary antibody for 32 minutes, and Ventana ultraView Universal DAB detection. Dual-color FISH for EGFR and CEP7 or PTEN and CEP10 was performed on 5-micron thick FFPE whole sections as previously described (17 (link)).
Solomon D.A., Wood M.D., Tihan T., Bollen A.W., Gupta N., Phillips J.J, & Perry A. (2015). Diffuse Midline Gliomas with Histone H3‐K27M Mutation: A Series of 47 Cases Assessing the Spectrum of Morphologic Variation and Associated Genetic Alterations. Brain Pathology, 26(5), 569-580.
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4

HER2 Immunohistochemistry Staining Protocol

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Freshly cut 4 μm sections of formalin fixed paraffin embedded (FFPE) cell line pellets and breast carcinoma tissue with known HER2 score were stained for HER2 antibody (Cell Signaling Technology; clone D8F12) at 0.3 μg/ml on a Ventana Benchmark XT platform using CC1 mild antigen retrieval and UltraView Universal DAB detection (Ventana). Stained slides were scanned and images were taken using gslideViewer software (Genentech).
Rost S., Giltnane J., Bordeaux J.M., Hitzman C., Koeppen H, & Liu S.D. (2017). Multiplexed ion beam imaging analysis for quantitation of protein expression in cancer tissue sections. Laboratory investigation; a journal of technical methods and pathology, 97(8).
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