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Anti cys no antibody

Manufactured by Merck Group
Sourced in United States

The Anti-Cys NO antibody is a laboratory reagent used in research applications. It is designed to detect and measure the presence of cysteine-nitrosothiols (Cys-NO), which are important post-translational modifications involved in cellular signaling and homeostasis. The antibody can be utilized in various research techniques, such as Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA), to study the role of Cys-NO in different biological systems.

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3 protocols using anti cys no antibody

1

Antibody Assay for RyR, Cys-NO, Calstabin, and Oxidative Stress Markers

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Antibodies: Anti-RyR (Affinity Bioreagents, cat. no. MA3-916, Golden, CO, USA; 1:2,000), Anti-RyR1 5029 (custom rabbit polyclonal antibody raised against the C-terminus of human RyR1, Yenzyme, South San Francisco, CA, USA; 1:250 for IP), anti-Cys NO antibody (Sigma, cat. no. N0409, St. Louis, MO, USA; 1:2,000), anti-calstabin antibody (Santa Cruz Biotechnology, cat. no. sc-6173, Santa Cruz, CA, USA, 1:2,500), anti-DNP (Oxyblot, cat. no. S7150, Millipore, Darmstadt, Germany; 1:250), anti-pSMAD3 (Abcam, cat. no. ab40854, Cambridge, UK; 1:1,000), anti-SMAD3 (Abcam, cat. no. 52903, Cambridge, UK; 1:1,1000), and anti-Nox4 (Abcam, cat. no. 109225, Cambridge, UK; 1:1,000).
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2

Investigating TGF-β1 Signaling Pathways

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Recombinant human TGF-β1 was purchased from R&D Systems. SD-208 was obtained from Epichem Pty Ltd (Bentley, WA). Zoledronic acid (Zometa) was obtained from Novartis (Cambridge, MA). Rycal S107 (S107-HCl, FW 245.77) was synthesized as previously described17 (link),37 (link). Anti-TGF-β antibody clone 1D11.16.8 (1D11) and mouse IgG1 isotype control (MOPC-21; unknown specificity) were obtained from BioXCell (West Lebanon, NH). Nox4 inhibitor (GKT137831) was obtained from Ark Pharm, Inc. (Libertyville, IL). Antibodies: Anti-RyR (Affinity Bioreagents, cat. MA3-916, Golden, CO; 1:2,000), anti-Cys NO antibody (Sigma, cat. N0409, St. Louis, MO; 1:2,000), anti-calstabin antibody (Santa Cruz Biotechnology, cat. sc-6173, Santa Cruz, CA; 1:2,500), anti-DNP (Oxyblot, Millipore, Darmstadt, Germany; 1:250), anti-pSMAD3 (Abcam, cat. ab40854, Cambridge, UK; 1:1000), anti-SMAD3 (Abcam, cat. 52903, Cambridge, UK; 1:1000), anti-Nox4 (Abcam, cat. 109225, Cambridge, UK; 1:1000), anti-GAPDH (Sigma, cat. G8795, St. Louis, MO; 1:500) and anti-tubulin (Sigma, cat. 8203, St. Louis, MO; 1:500 each). Fluo-4 AM and Fura-red were obtained from Invitrogen/Molecular probes (cat. F-14201 and cat. F-3020, Eugene, OR).
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3

Investigating TGF-β1 Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human TGF-β1 was purchased from R&D Systems. SD-208 was obtained from Epichem Pty Ltd (Bentley, WA). Zoledronic acid (Zometa) was obtained from Novartis (Cambridge, MA). Rycal S107 (S107-HCl, FW 245.77) was synthesized as previously described17 (link),37 (link). Anti-TGF-β antibody clone 1D11.16.8 (1D11) and mouse IgG1 isotype control (MOPC-21; unknown specificity) were obtained from BioXCell (West Lebanon, NH). Nox4 inhibitor (GKT137831) was obtained from Ark Pharm, Inc. (Libertyville, IL). Antibodies: Anti-RyR (Affinity Bioreagents, cat. MA3-916, Golden, CO; 1:2,000), anti-Cys NO antibody (Sigma, cat. N0409, St. Louis, MO; 1:2,000), anti-calstabin antibody (Santa Cruz Biotechnology, cat. sc-6173, Santa Cruz, CA; 1:2,500), anti-DNP (Oxyblot, Millipore, Darmstadt, Germany; 1:250), anti-pSMAD3 (Abcam, cat. ab40854, Cambridge, UK; 1:1000), anti-SMAD3 (Abcam, cat. 52903, Cambridge, UK; 1:1000), anti-Nox4 (Abcam, cat. 109225, Cambridge, UK; 1:1000), anti-GAPDH (Sigma, cat. G8795, St. Louis, MO; 1:500) and anti-tubulin (Sigma, cat. 8203, St. Louis, MO; 1:500 each). Fluo-4 AM and Fura-red were obtained from Invitrogen/Molecular probes (cat. F-14201 and cat. F-3020, Eugene, OR).
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