C57bl 6j wt mice

All animal experiments were approved by the Institutional Animal Care and Use Committee of The University of Tokyo (Approved No. P23-066). Mice were housed in 12-12 h light-dark cycles. C57BL/6J WT mice were purchased (CLEA Japan Inc., Tokyo, Japan). L-PGDS-deficient (L-pgds^−/−) mice, H-PGDS-deficient (H-pgds^−/−) mice, DP1-deficient (Dp1^−/−) mice, and DP2-deficient (Dp2^−/−) mice were generated as previously described (16 (link), 17 (link), 18 (link), 19 (link)). All mice were C57BL/6J background.
P1 neonatal mice were administrated with rat anti-PDGFRβ antibody (APB5, intraperitoneally, 50 μg/50 μl in PBS; purified as previously described (6 )). The control mice were administrated with only PBS. After the administration, mice were euthanized by cervical dislocation, and the eyes were excised and analyzed for further experiments.

C57BL/6J (WT) mice were purchased from Clea Japan (Tokyo, Japan). CXCR3KO mice (B6.129P2-Cxcr3tm1Dgen/J, Stock Number: 005796) were purchased from the Jackson Laboratory (Bar Harbor, ME). The CXCR3KO mice have been backcrossed to C57BL/6 at least six times before being imported to our laboratory. All animals were housed in cages (<6 mice/cage, 225 mm × 340 mm × 155 mm) containing wood chip bedding under specific-pathogen-free conditions in the animal facility of the National Research Center for Protozoan Diseases at Obihiro University of Agriculture and Veterinary Medicine. Male mice at 9–11 weeks old were used for in vivo experimental infection and preparation of peritoneal macrophages. The mean ± SD starting weight was 23.6 ± 1.0 g for WT mice and 23.7 ± 1.3 g for CXCR3KO mice. Mice at 6–8 weeks old were used as the parents of fetal mice for the preparation of primary glial cells.