Cells were inoculated in 96-well plates with 1 × 104 cells per well. 10 μl of EdU (50 μM) was applied in each well for cell labeling. Two hours later, cells were incubated with 4% methanol for 20 min, followed by PBS washing and incubation with Cell-Light™EdU Apollo®488 (Life Technologies, New York, USA). 4′,6-Diamidino-2-phenylindole (DAPI) was used for nuclei staining in the dark. Finally, cells were washed in PBS and captured.
Cell light edu apollo 488
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Cell-Light™EdU Apollo®488 is a fluorescent reagent used to detect and visualize newly synthesized DNA in proliferating cells. It functions by incorporating the thymidine analog EdU (5-ethynyl-2'-deoxyuridine) into DNA during the DNA synthesis phase of the cell cycle, which can then be detected using a fluorescent dye.
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2 protocols using cell light edu apollo 488
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Cell proliferation assay with EdU and DAPI
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Cell Proliferation Assay with EdU
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10 μL of EdU (50 μM) was applied for cell labeling. Two hours later, cells were incubated with 4% methanol for 20 min, followed by PBS washing and incubation with Cell-Light™EdU Apollo®488 (Life Technologies, New York, NY, USA). 4′,6-Diamidino-2-phenylindole (DAPI) was used for nuclei staining in the dark. Finally, cells were washed in phosphate buffered saline (PBS) and captured for calculating EdU-positive rate.
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