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Anti k11 ubiquitin

Manufactured by Merck Group

Anti-K11 ubiquitin is a laboratory reagent used in research applications. It is a specific antibody that recognizes and binds to the K11 linkage of ubiquitin, a small protein involved in cellular protein regulation. This product can be used to detect and study the K11-linked ubiquitination of proteins.

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2 protocols using anti k11 ubiquitin

1

Evaluating Ubiquitin Linkage Profiles

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Proteins were separated by standard 10% SDS-polyacrylamide gel electrophoresis (SDS–PAGE) loaded in Laemmli buffer containing 10 mM DTT. Samples were transferred to PVDF membrane and immunoblotting was performed using the following antibodies: anti-K63 ubiquitin (1:4,000 - cat# 05-1308, clone apu3, EMD Millipore), anti-K11 ubiquitin (1:1,000 - cat# MABS107, clone 2A3/2E6 EMD Millipore), anti-K48 (1:10,000 - cat# 4289, clone D9D5 - Cell Signaling), anti-actin (1:5,000 - cat# 4967 - Cell Signaling), anti-GAPDH (1:4,000 - cat# ab9485, Abcam), anti-DNP (1:8,000 - cat# D9656 - Sigma). Anti-mouse and anti-rabbit secondary antibodies conjugated with HRP and ECL prime detection reagents were acquired from GE Healthcare Life Sciences. All antibodies have been validated by the manufacturer or are expected to react with the species used in this study based on sequence similarity.
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2

Evaluating Ubiquitin Linkage Profiles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were separated by standard 10% SDS-polyacrylamide gel electrophoresis (SDS–PAGE) loaded in Laemmli buffer containing 10 mM DTT. Samples were transferred to PVDF membrane and immunoblotting was performed using the following antibodies: anti-K63 ubiquitin (1:4,000 - cat# 05-1308, clone apu3, EMD Millipore), anti-K11 ubiquitin (1:1,000 - cat# MABS107, clone 2A3/2E6 EMD Millipore), anti-K48 (1:10,000 - cat# 4289, clone D9D5 - Cell Signaling), anti-actin (1:5,000 - cat# 4967 - Cell Signaling), anti-GAPDH (1:4,000 - cat# ab9485, Abcam), anti-DNP (1:8,000 - cat# D9656 - Sigma). Anti-mouse and anti-rabbit secondary antibodies conjugated with HRP and ECL prime detection reagents were acquired from GE Healthcare Life Sciences. All antibodies have been validated by the manufacturer or are expected to react with the species used in this study based on sequence similarity.
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