Eight weeks old C57BL/6 male mice (weighing 20–22g) and matched ALDH2 knockout (KO) male mice were used in our study. Among them, C57BL/6 mice were obtained from Shanghai Jiesijie Laboratory Animal Centre (Shanghai, China) and ALDH2 KO mice were generated as described previously [58] (link). All animals experiment was performed according to institutional guidelines. Bone marrow cells were isolated from 8-weeks-old C57BL/6 wild type and ALDH2 KO male mice respectively. Unpurified mononuclear cells (MNCs) were separated by Ficoll-hypaque gradient centrifugation, the representative buffy coat was collected and washed by stain buffer for eliminating the Ficoll-hypaqyue solution. Finally, cells were stained with Aldefluor reagent and ALDH inhibitor DEAB (Stem Cell Technologies), then ALDHbr cells with high ALDH activity and low side scatter were gated and collected by fluorescence-activated cell sorting FACS Calibur (BD biosciences).
Aldh inhibitor deab
Manufactured by STEMCELL
DEAB is an aldehyde dehydrogenase (ALDH) inhibitor. ALDH enzymes play a role in the oxidation of aldehydes. DEAB functions as an ALDH inhibitor.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using aldh inhibitor deab
1
Isolation of ALDH-bright Murine Bone Marrow Cells
2
Measuring ALDH Activity in LP Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The aldehyde dehydrogenase (ALDH) activity of the LP cells was detected using an ALDEFLUOR staining kit (StemCell Technologies, Canada). Briefly, the LP cells in ALDEFLUOR buffer containing ALDH substrate were incubated at 37°C for 45 min in the dark. After washing with the buffer and centrifugation, the cell pellet was suspended in ice-cold ALDEFLUOR buffer, add and incubate with biotin-conjugated monoclonal anti-CD11c, anti-CD11b and anti-Siglec-F, and then detected by flow cytometry[15 (link)]. The ALDH activity was measured by the difference values of mean fluorescence intensity (MFI) of the LP cells before or after treatment with 300 μM ALDH inhibitor DEAB (StemCell Technologies).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!