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Sj l thy1.1

Manufactured by Jackson ImmunoResearch

SJ/L (Thy1.1) is a mouse monoclonal antibody that recognizes the Thy1.1 antigen, also known as CD90.1. It is a cell surface glycoprotein expressed on a subset of T cells in certain mouse strains. This antibody can be used for the identification and isolation of Thy1.1-positive cells.

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3 protocols using sj l thy1.1

1

Murine Model Immunology Protocols

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Animal work was done in accordance with the Institutional Animal Care and Use Committee of the University of Pittsburgh. All mice were on C57BL/6 background, used at 6–8 weeks of age, were both male and female, and housed in specific pathogen free conditions prior to use. C57BL/6, SJ/L (Thy1.1), Cd4Cre, Tg(TcraTcrb)1100Mjb/J (OT-I), B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J (Pmel), and HIF1af/f mice were obtained from The Jackson Laboratory. Prdm1f/f mice were a gift from A. Poholek (University of Pittsburgh). E8I-CreERT2 GFP x Rosa26-LSL-TdTomato mice and spleens from Tg(Nr4a1-EGFP/cre)820Khog/J (Nur77-GFP reporter) mice were a gift from D.A.A. Vignali (University of Pittsburgh).
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2

Murine Experiments in Controlled SPF

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All animal work was done in accordance with the Institutional Animal Care and Use Committee of the University of Pittsburgh. All mice were housed in specific pathogen free conditions. 6–8 week mice of similar weight and mixed genders were randomized prior to experimentation. C57/BL6, SJ/L (Thy1.1), and OT-I mice were obtained from The Jackson Laboratory.
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3

Murine Model Immunology Protocols

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Animal work was done in accordance with the Institutional Animal Care and Use Committee of the University of Pittsburgh. All mice were on C57BL/6 background, used at 6–8 weeks of age, were both male and female, and housed in specific pathogen free conditions prior to use. C57BL/6, SJ/L (Thy1.1), Cd4Cre, Tg(TcraTcrb)1100Mjb/J (OT-I), B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J (Pmel), and HIF1af/f mice were obtained from The Jackson Laboratory. Prdm1f/f mice were a gift from A. Poholek (University of Pittsburgh). E8I-CreERT2 GFP x Rosa26-LSL-TdTomato mice and spleens from Tg(Nr4a1-EGFP/cre)820Khog/J (Nur77-GFP reporter) mice were a gift from D.A.A. Vignali (University of Pittsburgh).
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