Full length htt mab2166

Protein phosphatase inhibitor and protease inhibitor cocktails were purchased from Sigma-Aldrich. VCP inhibitor Eer I and proteasome inhibitor MG132 were from Tocris Bioscience. Antibodies for Tom20 (sc-11415, 1:1,000), c-Myc (sc-40, 1:1,000), GFP (sc-9996, 1:1,000), GST (sc-138, 1:500), CD3 (sc-20047, 1:500), Enolase (sc-15343, 1:1,000), Tim23 (sc-514463, 1:500) and Parkin (sc-32282, 1:1,000) were from Santa Cruz Biotechnology. Full-length Htt (MAB2166, 1:1,000), polyQ (MAB1574, 1:1,000), EM48 (MAB5374, 1:1,000) and NeuN (MAB377, 1:500) antibodies were from Millipore. Pan-actin (A1978, 1:10,000) and Flag (F3165, 1:5,000) antibodies were from Sigma-Aldrich. Antibodies for VDAC (ab14734, 1:2,000), Clpp (ab124822, 1:1,000), UBXD1 (ab103651, 1:500) and VCP (ab109240, 1:10,000) were from Abcam. EEA1 (3288, 1:500) and LC3 (2775, 1:1,000) antibodies were from Cell Signalling, WFS1 (NB100-1918, 1:1,000) antibody was from Novus, HMGB1 (10829-1-AP, 1:1,000) antibody was from Proteintech, and GRP78 (ADI-SPA-826, 1:1,000) and Calnexin (ADI-SPA-860, 1:1,000) antibody was from Enzo Life Sciences. Anti-mouse IgG and anti-rabbit IgG, peroxidase-linked, species-specific antibodies were from Thermo Scientific.