Retinas and sclera‐choroid‐RPE cell complexes of non‐burned (D0) and burned mice at D3 were cut into small pieces and homogenized in a solution of 750 U/ml DNAse I (Sigma‐Aldrich Corp.) and 0.5 mg/ml of collagenase (Roche) for 20 min at 37°C. Homogenates were filtered through a 70 μm cell strainer and washed in PBS. Viability of the cells was checked by Zombie Aqua (423101: BioLegend) staining for 15 min at room temperature. After incubation with LEAF‐purified anti‐mouse CD16/32 (101310; BioLegend) for 10 min at 4°C to block Fc receptors, cells were incubated for 25 min at 4°C with the following antibodies: BV711 anti‐mouse/human CD11b (101242; BioLegend), PE anti‐mouse F4/80 (123110; BioLegend), APC anti‐mouse CD64 (139305; BioLegend), FITC anti‐mouse CD38 (102705; BioLegend), APC/Cy7 anti‐mouse Ly‐6G (127624; BioLegend), BV785 anti‐mouse CD11c (117335; BioLegend), and PE/Cy7 anti‐mouse CD206 (141719; BioLegend). Antibody dilution was determined with titration by lot. Fluorescence‐activated cell sorting (FACS) was performed on a BD LSRFortessaTM X‐20 cell analyzer, and data were analyzed using FlowJo software (FlowJo version 10.2).
Bv785 anti mouse cd11c
Manufactured by BioLegend
BV785 anti-mouse CD11c is a fluorochrome-conjugated monoclonal antibody that binds to the CD11c antigen, which is expressed on the surface of mouse dendritic cells and certain other myeloid cells. This product is intended for use in flow cytometry applications to identify and characterize CD11c-positive cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Zombie aqua, by BioLegend (2 mentions)
Pe anti mouse f4 80, by BioLegend (2 mentions)
Bv711 anti mouse human cd11b, by BioLegend (2 mentions)
Apc cy7 anti mouse ly 6g, by BioLegend (2 mentions)
Leaf purified anti mouse cd16 32, by BioLegend (2 mentions)
Apc anti mouse cd64, by BioLegend (2 mentions)
Fitc anti mouse cd38, by BioLegend (2 mentions)
Pe cy7 anti mouse cd206, by BioLegend (2 mentions)
Dnase 1, by Merck Group (1 mentions)
Collagenase, by Roche (1 mentions)
Lsrfortessa x 20 cell analyzer, by BD (1 mentions)
2 protocols using bv785 anti mouse cd11c
1
Characterization of Murine Retinal Cell Types
2
Multicolor Flow Cytometry of BMDMs
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BMDMs were collected in PBS through scraping. Viability of the cells was checked by Zombie Aqua (423101: BioLegend) staining for 15 min at room temperature. After incubation with LEAF‐purified anti‐mouse CD16/32 (101310; BioLegend) for 10 min at 4°C to block Fc receptors, cells were incubated for 25 min at 4°C with the following antibodies: BV711 anti‐mouse/human CD11b (101242; BioLegend), PE anti‐mouse F4/80 (123110; BioLegend), APC anti‐mouse CD64 (139305; BioLegend), FITC anti‐mouse CD38 (102705; BioLegend), APC/Cy7 anti‐mouse Ly‐6G (127624; BioLegend), BV785 anti‐mouse CD11c (117335; BioLegend), and PE/Cy7 anti‐mouse CD206 (141719; BioLegend). Antibody dilution was determined with titration by lot. Fluorescence‐activated cell sorting (FACS) was performed on a BD LSRFortessaTM X‐20 cell analyzer, and data were analyzed using FlowJo software (FlowJo version 10.2).
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