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Magpure ffpe dna kit b

Manufactured by Magen Biotechnology Co
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Sourced in China

The MagPure FFPE DNA Kit B is a laboratory equipment product designed for the extraction and purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. The kit utilizes magnetic bead technology to isolate high-quality DNA, which can be used in various downstream applications.

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Lab products found in correlation

Qubit dsdna hs high sensitivity assay kit, by Thermo Fisher Scientific (3 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (3 mentions) Tguide s32 magnetic blood genomic dna kit, by Tiangen Biotech (3 mentions) Magmax cell free dna cfdna isolation kit, by Thermo Fisher Scientific (1 mentions) Novaseq 6000 system, by Illumina (1 mentions) Universal dna library prep kit, by Vazyme (1 mentions) Hypercap target enrichment kit, by Roche (1 mentions) Le220, by Covaris (1 mentions) Kapa hyper preparation kit, by Roche (1 mentions) Accugreen high sensitivity dsdna quantitation kit, by Biotium (1 mentions)

Spelling variants of this product

MagPure FFPE DNA LQ kit B (2 citations)

4 protocols using magpure ffpe dna kit b

1

Efficient DNA Extraction Protocols

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The genomic DNA of PBL was extracted using the TGuide S32 Magnetic Blood Genomic DNA Kit (Tiangen). cfDNA in plasma was extracted using the MagMAX Cell‐Free DNA (cfDNA) Isolation kit (Thermo Fisher Scientific). The genomic DNA of tumour tissue was extracted from formalin‐fixed paraffin‐embedded (FFPE) samples using the MagPure FFPE DNA Kit B (Magen). The concentration of extracted DNA was measured using the Qubit dsDNA HS (High Sensitivity) Assay Kit (Thermo Fisher Scientific), and the quality of extracted DNA was assessed using the Agilent 2100 BioAnalyzer (Agilent).
Xu E., Su K., Zhou Y., Gong L., Xuan Y., Liao M., Cao J., Li Y., Lu Y., Zhao Y, & Chen F. (2021). Comprehensive landscape and interference of clonal haematopoiesis mutations for liquid biopsy: A Chinese pan‐cancer cohort. Journal of Cellular and Molecular Medicine, 25(21), 10279-10290.
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2

DNA Extraction from FFPE and PBL Samples

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Genomic DNA of tumor was extracted from formalin-fixed paraffin-embedded (FFPE) samples using MagPure FFPE DNA Kit B (Magen, China, ID: D6323-02). Genomic DNA of peripheral blood lymphocyte (PBL) was extracted using TGuide S32 Magnetic Blood Genomic DNA Kit (Tiangen, China, ID: DP601). The concentration of DNA was measured by Qubit dsDNA HS (High Sensitivity) Assay Kit (Thermo Fisher, USA, ID: Q32851), while the quality of DNA was assessed by Agilent 2100 BioAnalyzer (Agilent, USA). All samples for DNA extraction were obtained before the treatments started.
Zhan Q., Wen C., Zhao Y., Fang L., Jin Y., Zhang Z., Zou S., Li F., Yang Y., Wu L., Jin J., Lu X., Xie J., Cheng D., Xu Z., Zhang J., Wang J., Deng X., Chen H., Peng C., Li H., Zhang H., Fang H., Wang C, & Shen B. (2021). Identification of copy number variation-driven molecular subtypes informative for prognosis and treatment in pancreatic adenocarcinoma of a Chinese cohort. EBioMedicine, 74, 103716.
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3

Comprehensive Genomic Profiling of FFPE Tumors

Cited 1 time
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Detail methods of DNA sequencing was performed as our previous study [12 (link)]. DNA panel was 2,189 kb in length, which covered full coding regions or hotspot mutation regions in 769 genes. The genomic DNA from formalin-fixed paraffin-embedded (FFPE) tumor samples was extracted using the MagPure FFPE DNA Kit B (Magen, China), fragmented into DNA pieces of approximately 200 bp using an enzymatic method (5 × FEA Enzyme Mix; Qiagen, CN), subsequently constructed DNA libraries using a VAHTS Universal DNA Library Prep Kit (Vazyme, China). The libraries of genomic DNA were captured with a 769 gene panel (Table S1) and sequenced using a HyperCap Target Enrichment Kit (Roche, Switzerland) and the NovaSeq 6000 system (Illumina, USA) according to the manufacturer’s protocols, respectively.
Pang W., Gong L., Shi W., Zheng H., Ye M., Chen J., Li R., Zhang X., Ren D, & Wang Z. (2024). Identification of gene variation feature for targeted therapy of non-small cell lung cancer through combined method of DNA and RNA sequencing. Discover. Oncology, 15, 67.
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4

FFPE DNA Extraction and Library Prep

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Genomic DNA was extracted from FFPE samples using the MagPure FFPE DNA Kit B (Magen, China). Peripheral blood lymphocyte (PBL) DNA was extracted by the TGuide S32 Magnetic Blood Genomic DNA Kit (Tiangen, China). The DNA concentration was measured by the Qubit dsDNA HS (High Sensitivity) Assay Kit (Thermo Fisher, USA). The quality of DNA was assessed by an Agilent 2100 BioAnalyzer (Agilent, USA). DNA (50-300 ng) from FFPE samples was sheared with Covaris LE220 according to the recommended settings for ~200 bp fragments, and the fragmented DNA was input for library construction by the KAPA Hyper Preparation Kit (Kapa Biosystems, USA) according to the manufacturer's instructions. All libraries were quantified by the AccuGreen High Sensitivity dsDNA Quantitation Kit (Biotium, USA), and the size of the libraries was determined on the Agilent Bioanalyzer 2100 (Agilent, USA).
Ju S., Cui Z., Hong Y., Wang X., Mu W., Xie Z., Zeng X., Su L., Zhang Q., Song X., You S., Chen R., Chen W., Xuchun, & Zhao J. (2021). Detection of Multiple Types of Cancer Driver Mutations Using Targeted RNA Sequencing in NSCLC.
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