Cryovial

Manufactured by Corning

Sourced in United States

Cryovials are specialized laboratory containers designed for the safe storage and preservation of samples and specimens at ultra-low temperatures, typically in cryogenic freezers or liquid nitrogen storage systems. They are constructed of high-quality materials to provide reliable performance and protection for the stored contents.

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Lab products found in correlation

34 protocols using cryovial

Anaerobic Fecal Microbiome Sample Preparation

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The donor stool sample was weighed and then moved into an anaerobic chamber (AS-580, Anaerobe Systems, Morgan Hill, CA) containing a gas mixture of 4.6% CO2 / 5% H2 / 90.4% N2 as soon as possible after collection (within 30 minutes for donor 1, 60 minutes for donor 3, and 4 hours for donor 2). Inside the anaerobic chamber, the stool was transferred to a Ninja 400-watt blender (QB900B Shark Ninja, Needham, MA). Chilled (4 o C) and pre-reduced anaerobically sterilized (PRAS) dilution blank medium (AS-9183, Anaerobe Systems) was added at a volume of 2:1 (2mL per gram) of the stool weight. The sample was blended until fully homogenized (approximately 4 minutes). Three 0.5mL aliquots of homogenized stool were collected into 2mL cryovials (#431386, Corning Life Sciences, Corning, NY ) and immediately frozen at -80 o C. One cryovial was sent for WGS sequencing and analysis, the others were maintained in frozen storage.
A sequential dilution of homogenized stool into PRAS Yeast Casitone Fatty Acid with Carbohydrate (YCFAC) broth (AS-680, Anaerobe Systems) was performed to obtain a 10 -5 dilution, which was used for high density growth and in serial dilutions for low density growth on solid media as described below.

Hyde E.R., Lozano H., & Cox S. (2020). BIOME-Preserve: A Novel Storage and Transport Medium for Preserving Anaerobic Microbiota Samples for Culture Recovery.

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Oral Gavage of Murine Fecal Microbiome

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For human fecal challenge experiments, a fecal mixture was defrosted in the anaerobic chamber and diluted 1:100 into pre-reduced PBS. One milliliter was aliquoted into pre-reduced 2-mL Corning cryovials, removed from the anaerobic chamber, and transported to the vivarium, where each vial was uncapped and orally gavaged into mice within 1 min of uncapping. Each mouse received 200 μL of the bacterial mixture. Feces contains ~10¹¹ colony forming units per gram of feces (Vandeputte et al. 2017 (link)); based on the dilutions performed, we estimate that each mouse received 10⁸-10¹⁰ bacterial cells in the fecal challenge.
For all non-challenge fecal colonization experiments, the preserved fecal mixture was defrosted in the anaerobic chamber and diluted 1:2 into pre-reduced PBS. One millilter of the resulting mixture was aliquoted into pre-reduced 2-mL Corning cryovials, removed from the anaerobic chamber, and transported to the vivarium, where each vial was uncapped and orally gavaged into mice within 1 min of uncapping. Each mouse received 200 μL of the bacterial mixture, equivalent to 10¹⁰–10¹¹ bacterial cells per mouse.

Cheng A.G., Ho P.Y., Aranda-Díaz A., Jain S., Yu F.B., Meng X., Wang M., Iakiviak M., Nagashima K., Zhao A., Murugkar P., Patil A., Atabakhsh K., Weakley A., Yan J., Brumbaugh A.R., Higginbottom S., Dimas A., Shiver A.L., Deutschbauer A., Neff N., Sonnenburg J.L., Huang K.C, & Fischbach M.A. (2022). Design, construction, and in vivo augmentation of a complex gut microbiome. Cell, 185(19), 3617-3636.e19.

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Cryopreservation and Culture of DPSCs

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The CD146⁺ DPSCs (P3) in residual T75 flasks were harvested and re‐suspended with freezing medium, consisting of 10% dimethyl sulphoxide (DMSO, Sigma‐Aldrich, Steinheim, Germany) and 90% FBS. 1 × 10⁶ cells were aliquoted into cryovials (Corning, Lowell, MA) and stored in the Nalgene^™ Cryo 1°C Freezing Container (Thermo Fisher Scientific, USA), then kept in −80°C Freezer overnight for gradient cooling enabling a cooling rate at −1°C/min. Finally, they were transferred into liquid nitrogen.
In 3 months, CD146⁺ DPSCs were thawed in water bath at 37°C and seeded in 96‐well plates (2 × 10³ cells per well), marked as P4. Complete culture medium was used as the control medium (CM). 5 ng/mL bFGF was chosen as the minimal concentration in this experiment according to the literature, which showed highly proliferative response from bone marrow mesenchymal stem cells (BMMSCs).³¹ Moreover, a study had been reported that 100 ng/mL bFGF had obvious impact on the proliferation of dental pulp cells.³² Thus, in experiment groups, recovered DPSCs (P4) were immediately supplemented with 5, 10, 20, 50, 80 and 100 ng/mL bFGF. Fresh medium with bFGF was changed every 2 days.

Luo L., Zhang Y., Chen H., Hu F., Wang X., Xing Z., Albashari A.A., Xiao J., He Y, & Ye Q. (2020). Effects and mechanisms of basic fibroblast growth factor on the proliferation and regenerative profiles of cryopreserved dental pulp stem cells. Cell Proliferation, 54(2), e12969.

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Cryopreservation of Semen Samples

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Semen samples were cryopreserved using the TEST-Yolk Buffer (TYB, Irvine Scientific, Santa Ana, CA, USA). Aliquots of TYB equal to 25% of the sample volume were added to the specimen at room temperature, and mixed gently for 5 min using the Hema-Tek aliquot mixer (Miles Scientific, Elkhart, IN, USA). The procedure was repeated to a final 1:1 (v/v) ratio of the freezing medium to the sample. Further, the samples were dispensed into cryovials (1.5 mL; Corning, Pittsburg, PA, USA) and transferred to the freezer (−20 °C) for 8 min (static cooling), and subsequently to liquid nitrogen vapor (−80 °C) for 2 h (vapor-phase cooling). Finally, the cryovials were stored in liquid nitrogen at −196 °C until use for proteomics analysis.

Panner Selvam M.K., Finelli R., Baskaran S, & Agarwal A. (2020). Dysregulation of Key Proteins Associated with Sperm Motility and Fertility Potential in Cancer Patients. International Journal of Molecular Sciences, 21(18), 6754.

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Cryopreservation of Platelet-Lysate Mesenchymal Stem Cells

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At the fifth subculture, PL-MSCs were harvested at 90% confluency, centrifuged (200 g/5 min), resuspended in cryopreservation media (DMEM-LG + 5% hPL + 10% DMSO (CryoMACS DMSO, MiltenyiBiotec, Germany)), and aliquoted into 2 ml cryovials (Corning, USA). Mr. Frosty container (Nalgene, Thermo Fisher Scientific, USA) was applied for slow freezing of samples according to the manufacturer’s instruction. Then, the cryopreserved PL-MSCs were transferred to the vapor phase of the liquid nitrogen tank (Statebourne Cryogenics, UK).

Aghayan H.R., Salimian F., Abedini A., Fattah Ghazi S., Yunesian M., Alavi-Moghadam S., Makarem J., Majidzadeh-A K., Hatamkhani A., Moghri M., Danesh A., Haddad-Marandi M.R., Sanati H., Abbasvandi F., Arjmand B., Azimi P., Ghavamzadeh A, & Sarrami-Forooshani R. (2022). Human placenta-derived mesenchymal stem cells transplantation in patients with acute respiratory distress syndrome (ARDS) caused by COVID-19 (phase I clinical trial): safety profile assessment. Stem Cell Research & Therapy, 13, 365.

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Cryopreservation of Single Cells with Antioxidants and Apoptosis Inhibitors

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After culture, single cells, recovered by trypsinization, were suspended at 2.5 × 10⁵ cells/mL of freezing medium and placed in 1.8-mL cryovials (Corning). cryovials were frozen in a Nalgene freezing container at a rate of -1°C per minute to -80°C and stored overnight at -80°C. After overnight storage, cryovials were placed in liquid nitrogen for long-term storage (at least one month). Cryopreservation stock medium, hereafter referred to as basal freezing medium, consisted of DPBS containing 10% fetal bovine serum (FBS) and10% DMSO (v/v). Treatment media was generated by mixing aqueous solutions (containing double the final concentration of cryoprotectant in DPBS) 1:1 with freezing medium containing 20% FBS and 20% DMSO resulting in treatment media containing the appropriate concentration of cryoprotectant in basal freezing medium (10% FBS and 10% DMSO). Cryopreservation agents, included five antioxidants, ascorbic acid (0.1, 0.5, 1 mM), glutathione (50, 100, 200 μM), hypotaurine (3.5, 7, 14 mM), glutathione peroxidase (1, 5, 10 U/mL), catalase (50, 100, 200 μg/mL), and two apoptosis inhibitors, Benzyloxycarbonyl-Val-Ala-DL-Asp-fluromethylketone (Z-VAD-fmk; 15, 30, 60 μM) and Trans-f-[(1R)-aminoethyl]-N-4-pyridinyl cyclohex anecar–boxamide dihydrochloride (Y-27632; 50, 100, 200 μM).

Ha S.J., Kim B.G., Lee Y.A., Kim Y.H., Kim B.J., Jung S.E., Pang M.G, & Ryu B.Y. (2016). Effect of Antioxidants and Apoptosis Inhibitors on Cryopreservation of Murine Germ Cells Enriched for Spermatogonial Stem Cells. PLoS ONE, 11(8), e0161372.

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Quantifying Molecule Localization and Clearance

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Two tracer molecules of widely separated molecular weights (10 kDa, 500 kDa) were used here to test if differences in spatial localization and systemic clearance of these sized molecules could be quantified with fluorescence detection as described here. Dextran-Texas Red (10,000 Da), dextran-fluorescein (500,000 Da), and Slide-A-Lyzer™ dialysis cassettes (3500 MWCO) were purchased from Life Technologies (Watham, MA). Both dextran-dye conjugates are expected to be of higher MW than reported due to the conjugated dye, by an amount that does not exceed 12% of the reported MW. CF™633 succinimidyl ester (CF633), sodium bicarbonate, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). Plasma collection tubes (EDTA-coated Vacutainer™, 4 mL) were ordered from Becton Dickinson (Franklin Lakes, NJ). Cryovials (2 mL) were purchased from Corning (Corning, NY) for storage of physiological fluids.

Mwangi T.K., Berke I., Nieves E.H., Bell R.D., Adams S.B, & Setton L.A. (2018). INTRA-ARTICULAR CLEARANCE OF LABELED DEXTRANS FROM NAIVE AND ARTHRITIC RAT KNEE JOINTS. Journal of controlled release : official journal of the Controlled Release Society, 283, 76-83.

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Biodistribution of Drugs in Mice

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Biodistribution studies were performed in healthy nude mice with intact BBB at two time points, 45 and 180 minutes after sonication. Following sonication, mice were injected i.v. with either ABX or CrEL-PTX (12 mg/kg). Forty-five minutes before euthanasia i.v. NaFl (Sigma-Aldrich) was administered at a dose of 20 mg/kg (Figure S1B). Dosing solutions were prepared by diluting a weighed amount of NaFl powder, ABX powder or measured volume of Taxol solution with 0.9% Saline solution to a final injection volume of 5 ml/kg. Mice were then euthanized with Euthasol (Virbac) solution (150 mg/kg, i.p.) and brains were carefully removed and imaged using Nikon AZ100 Epifluorescent microscope (4x, FITC filter cube, 2s exposure time). Highly fluorescent areas of the brain were separated from non-fluorescent regions using a clean No. 15 scalpel that was disposed of after every mouse. These samples were placed into separate cryo-vials (Corning) and flash frozen in liquid N₂. Heart, liver and plasma samples were also collected and immediately flash frozen in liquid N₂. Frozen tissue samples were stored in −80°C freezer for under 45 days before downstream PTX and NaFl concentration analysis.

Zhang D.Y., Dmello C., Chen L., Arrieta V.A., Gonzalez-Buendia E., Kane J.R., Magnusson L., Baran A., James C.D., Horbinski C., Carpentier A., Desseaux C., Canney M., Muzzio M., Stupp R, & Sonabend A.M. (2019). Ultrasound-mediated delivery of paclitaxel for glioma: a comparative study of distribution, toxicity and efficacy of albumin-bound versus cremophor formulations. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(2), 477-486.

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Cryogenic Preservation of Organoids

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Organoids were resuspended in freezing media (90% FBS/10% DMSO) in cryovials (Corning #375418), and immediately transferred to a CoolCell LX freezing container (Biocision #BCS-405G) and placed at −80° C for ≥ 2 hr.

Beshiri M.L., Tice C.M., Tran C., Nguyen H.M., Sowalsky A.G., Agarwal S., Jansson K.H., Yang Q., McGowen K.M., Yin J., Alilin A.N., Karzai F.H., Dahut W.L., Corey E, & Kelly K. (2018). A PDX/organoid biobank of advanced prostate cancers captures genomic and phenotypic heterogeneity for disease modeling and therapeutic screening. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(17), 4332-4345.

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Cryopreservation of Testicular Tissue

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Once collected, the pair of whole-testis was transported to the research laboratory on ice in saline or Hank’s Balanced Salt Solution (HBSS; GIBCO cat # 24020117) and processed within 1 hour of removal by surgery. Around 90% of each testis was divided into smaller portions (~500 mg – 1g each) using scissors and directly transferred into cryovials (Corning cat # 403659) in DMEM medium (Life Technologies cat # 11995073) containing 10% DMSO (Sigma-Aldrich cat # D8779), 15% fetal bovine serum/FBS (GIBCO cat # 10082147) and cryopreserved in Mr. Frosty container (Thermo Fisher Scientific cat #5100–0001) at a controlled slow rate, and stored at −80°C for overnight. cryovials were transferred to liquid nitrogen for long-term storage.

Guo J., Nie X., Giebler M., Mlcochova H., Wang Y., Grow E.J., Kim R., Tharmalingam M., Matilionyte G., Lindskog C., Carrell D.T., Mitchell R.T., Goriely A., Hotaling J.M, & Cairns B.R. (2020). The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty. Cell Stem Cell, 26(2), 262-276.e4.

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