Ab96885

Manufactured by Abcam

Ab96885 is a laboratory equipment product. It is a device designed for specific laboratory procedures or experiments. The core function of this product is to facilitate certain tasks or processes within a laboratory setting. No further details about its intended use or features are provided.

Automatically generated - may contain errors

Lab products found in correlation

Ab22595, by Abcam (1 mentions) Ab59479, by Abcam (1 mentions) Ab96931, by Abcam (1 mentions) Eclipse ni e microscope, by Nikon (1 mentions) Tecnai g2 spirit twin transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Eclipse ti microscope, by Nikon (1 mentions) Ab96879, by Abcam (1 mentions) Ab9026, by Abcam (1 mentions) Ab7800, by Abcam (1 mentions) Vectashield mounting medium with dapi h 1200, by Vector Laboratories (1 mentions) Dfc340 fx camera, by Leica camera (1 mentions)

2 protocols using ab96885

Immunofluorescence and Transmission Electron Microscopy of Cellular Structures

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunofluorescence, cells were fixed in 4% PFA in 1× PBS for 15 to 20 minutes and permeabilized for 5 minutes with 0.1% Triton X-100 in PBS. Cells were blocked for 30 minutes with 5% goat serum and then incubated with primary antibodies (CD63 1:200, ab59479, Abcam; GluN1 1:500, MAB363, Merk & Co, Kenilworth, New Jersey, United States or Calnexin 1:1000, ab22595, Abcam) at 4°C overnight. After washing, cells were incubated with 2.5 μg mL
⁻¹Dylight 488/594-conjugated secondary antibodies (ab96931 and ab96885, respectively; both from Abcam) for 3 hours. Giemsa staining was performed using the Cytopro autostainer (ELITech, Paris, France). Brightfield and immunofluorescence microscopy was conducted using an Eclipse Ni-E microscope (Nikon, Tokyo, Japan). Hoffman and phase contrast images were taken on an Eclipse Ti microscope (Nikon).
Transmission electron microscopy was done as previously described.
18 (link)
Briefly, cells were fixed with 0.2% glutaraldehyde and 2% PFA in White's saline. Sections were counterstained with uranyl acetate and examined with a Tecnai G2 Spirit Twin transmission electron microscope (FEI Company, Hillsboro, Oregon, United States).

Hearn J.I., Green T.N., Chopra M., Nursalim Y.N., Ladvanszky L., Knowlton N., Blenkiron C., Poulsen R.C., Singleton D.C., Bohlander S.K, & Kalev-Zylinska M.L. (2020). N -Methyl-D-Aspartate Receptor Hypofunction in Meg-01 Cells Reveals a Role for Intracellular Calcium Homeostasis in Balancing Megakaryocytic-Erythroid Differentiation. Thrombosis and Haemostasis, 120(4), 671-686.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Epidermal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following TPEF imaging, tissues were fixed to be stained with hematoxylin and eosin (H&E) or antibodies against keratin 10 (K10), keratin 14 (K14), involucrin (Inv), loricrin (Lor), and proliferation marker [proliferating cell nuclear antigen (PCNA)]. Antigen retrieval was performed by incubating sections in a citrate buffer (10 mmol/L citric acid, 0.05% Tween 20, pH 6.0) at 95°C for 20 minutes. Primary antibodies were used at the indicated dilutions: K10 (1:200, Abcam ab9026), K14 (1:200; Abcam ab7800), involucrin (1:200, Abcam ab53112), loricrin (1: 80, Sigma-Aldrich AV41738), and PCNA (1:250, Abcam ab29). Secondary antibodies were used at the indicated dilutions: goat anti-rabbit (1:200; Abcam ab96885) and goat anti-mouse (1:200, Abcam ab96879). Slides were mounted using Vectashield H-1200 Mounting Medium with DAPI (Vector Labs) and imaged with a Leica DFC340 FX camera.

Varone A., Xylas J., Quinn K.P., Pouli D., Sridharan G., McLaughlin-Drubin M.E., Alonzo C., Lee K., Münger K, & Georgakoudi I. (2014). Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues. Cancer research, 74(11), 3067-3075.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!