Goat anti rabbit conjugated with peroxidase

Ptac2S was prepared as previously reported [5 , 19 ]. Cisplatin was purchased from Sigma (Milan, Italy). RPMI 1640 medium, antibiotics, glutamine and foetal bovine serum were purchased from Celbio (Milan, Italy). Caspase -9 and -7, BAX, PARP-1, phospho p38MAPK, phospho JNK1/2 antibodies were obtained from Cell Signalling (Celbio, Milan, Italy). PKC isoforms and phospho ERK1/2 antibodies, goat anti-rabbit conjugated with peroxidase, as well as control antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All others reagents were from Sigma (Milan, Italy).

Ptac2S was prepared according to previously reported procedures [4 (link), 5 (link)]. CDDP was purchased from Sigma-Aldrich, Chemicals (Milan, Italy). RPMI 1640 medium, antibiotics, glutamine and foetal bovine serum were purchased from Celbio (Milan, Italy). Caspase -9, -7 and -3, Bax, Bcl-2, poly(ADP-ribose) polymerase-1 (PARP-1), were obtained from Cell Signalling Technology (Celbio, Milan, Italy). PKC isoforms antibodies, phospho-specific p-38MAPK and total (phosphorylated and unphosphorylated) p-38MAPK antibodies, anti-porin (i.e. anti-voltage-dependent anion selective channel 1, VDAC1), goat anti-rabbit conjugated with peroxidase, as well as control antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All others reagents were from Sigma-Aldrich (Milan, Italy).