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Architect i1000 analyzer

Manufactured by Abbott
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Sourced in Germany, United States

The Architect i1000 analyzer is a compact, fully automated immunoassay system designed for routine clinical laboratory testing. It is capable of performing a wide range of immunoassay tests, including those for hormones, therapeutic drug monitoring, and infectious disease diagnostics. The analyzer is equipped with advanced technological features to ensure efficient and reliable operation.

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Lab products found in correlation

Cobas c702 analyzer, by Roche (4 mentions) Cobas e602 analyzer, by Roche (4 mentions)

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Architect analyzer (56 citations) Architect i1000 (37 citations)

9 protocols using architect i1000 analyzer

1

Fasting Blood Sample Analysis

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EDTA blood samples were collected in a fasting state for measurement of creatinine (Cr), estimated glomerular filtration rate (eGFR), albumin, total cholesterol, triglycerides, low-density lipoprotein, high-density lipoprotein, and hemoglobin A1C (Hgb A1C) if patients were diabetic. Plasma was assayed for these analytes on the same day as collection by the SF VAMC lab per standard methodology (Beckman Coulter Analyzer). Serum was isolated at the same time points as described above and assayed for homocysteine on the same day as collection by the SF VAMC lab per standard methodology (Abbott Diagnostics Architect i1000 Analyzer, Lake Forest, IL).]
Nosova E.V., Chong K.C., Alley H.F., Harris W.S., Boscardin W.J., Conte M.S., Owens C.D, & Grenon S.M. (2014). Clinical Correlates of Red Blood Cell Omega-3 Fatty Acid Content in Male Veterans with Peripheral Arterial Disease. Journal of vascular surgery, 60(5), 1325-1331.
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2

Biomarker Measurement in Serum Samples

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All samples were obtained by venipuncture into serum monovette® and centrifuged at 2500 ×g at 20°C for 10 minutes. The aliquoted samples were stored at −80°C until analysis. After thawing the samples were mixed gently by inverting and centrifuged with 2500 ×g for 10 minutes.
Galectin-3 was measured with the Galectin-3 assay on an Architect i1000 analyzer (Abbott, Wiesbaden, Germany). The limit of blank for this assay was 0.8 ng/mL as described in the instructions for use [20 ]. NT-proBNP was measured with the proBNP II STAT assay on a cobas e 602 analyzer (Roche Diagnostics, Mannheim, Germany). The limit of detection (LoD) for this assay was 5 pg/mL [21 ]. Serum creatinine was measured with the Creatinine Jaffe Gen. 2 assay on a cobas c 702 analyzer (Roche Diagnostics, Mannheim, Germany).
Kim S.H., Behnes M., Natale M., Hoffmann J., Reckord N., Hoffmann U., Budjan J., Henzler T., Papavassiliu T., Borggrefe M., Bertsch T, & Akin I. (2016). Galectin-3 Reflects Mitral Annular Plane Systolic Excursion Being Assessed by Cardiovascular Magnetic Resonance Imaging. Disease Markers, 2016, 7402784.
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3

Standardized Biomarker Measurement in Serum

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All expressed biomarkers were measured in the serum of patients’ blood. All samples were obtained by venipuncture into serum monovettes® and centrifuged at 2000 g for 10 min at 20 °C. The aliquoted samples were cooled down with liquid nitrogen before being stored at − 80 °C until analysis. The complete processing was conducted within two hours after blood extraction. After thawing, the samples were mixed gently by inverting and centrifuged with 2500g for 10 min at 20 °C, respectively, 3000g for 30 min for hsTnI at 4 °C.
HsTnT was measured with the Troponin T hs STAT assay on a cobas e 602 analyzer (Roche Diagnostics, Mannheim, Germany). The limit of blank (LoB) for this assay was 3 ng/L and the limit of detection (LoD) was 5 ng/L as described in the instructions for use [15 ]. HsTnI was measured with the STAT High sensitivity Troponin-I assay on an Architect i1000 analyzer (Abbott, Wiesbaden, Germany). The LoB was 0.7–1.3 ng/L and the LoD was 1.1–1.9 ng/L for this assay as described in the instructions for use [16 ]. NT-proBNP was measured with the proBNP II STAT assay on a cobas e 602 analyzer (Roche Diagnostics, Mannheim, Germany). The LoD for this assay was 5 ng/L [17 ]. Creatinine was measured with the Creatinine Jaffe Gen.2 assay on a cobas c 702 analyzer (Roche Diagnostics, Mannheim, Germany).
Natale M., Behnes M., Kim S.H., Hoffmann J., Reckord N., Hoffmann U., Budjan J., Lang S., Borggrefe M., Papavassiliu T., Bertsch T, & Akin I. (2017). High sensitivity troponin T and I reflect mitral annular plane systolic excursion being assessed by cardiac magnetic resonance imaging. European Journal of Medical Research, 22, 38.
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4

Serum Biomarker Profile Assessment

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Fasting blood samples were collected for measuring serum 25(OH) D, albumin, iPTH, alkaline phosphate, plasma homocysteine levels, and lipid profiles. Serum 25(OH)D was measured using ELISA (Immunodiagnostic Systems Inc., Fountain Hills, AZ, USA) that detected both vitamins D2 and D3, with a detection limit of 5.0 nmol/L. Intact PTH was measured using an iPTH immunoradiometric assay (IRMA; Scantibodies Laboratory, Inc., Santee, CA, USA) with a normal range of 8–76 pg/mL. Serum cholesterol was determined with an enzymatic photometric assay using a Hitachi 7600 analyzer (Roche, Hitachi system, Tokyo, Japan). Serum high-density lipoprotein (HDL) and low-density lipoprotein (LDL) levels were determined using homogeneous enzymatic colorimetric assays (Sekisui, Osaka, Japan); the coefficient of variation (CV) was 2.08% and 2.61%, respectively. Serum triglycerides were assessed with an enzymatic photometric assay (CHO-HMMPS method) (WAKO, Osaka, Japan), with a CV of 1.98%. Serum homocysteine and vitamin B12 levels were determined using the chemiluminescent microparticle immunoassay method on an Architect i1000 analyzer (Abbott, MA, USA).
Ou S.H., Chen M.Y., Huang C.W., Chen N.C., Wu C.H., Hsu C.Y., Chou K.J., Lee P.T., Fang H.C, & Chen C.L. (2016). Potential Role of Vegetarianism on Nutritional and Cardiovascular Status in Taiwanese Dialysis Patients: A Case-Control Study. PLoS ONE, 11(6), e0156297.
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5

Biomarker Measurement Protocol for Clinical Samples

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Blood samples were collected from all patients (at rest) at a single assessment time point upon study inclusion by venepuncture with serum monovettes and centrifuged at 2,500 g at 20℃ for 10 minutes. The aliquoted samples were cooled down in liquid nitrogen before being stored at −80℃ for further analysis. After thawing, the samples were mixed gently by inverting and centrifuged at 2,500 g for 10 minutes at 20℃.
Gal-3 levels were assessed using the Gal-3 assay on an Architect i1000 analyzer (Abbott, Wiesbaden, Germany). The limit of blank for this assay was 0.8 ng/mL, as specified in the user instructions (Galectin-3, Architect System, © 2012, 2013 Abbott Laboratories). Serum creatinine concentrations were measured using the Creatinine Jaffe Gen.2 assay on a Cobas c 702 analyzer (Roche Diagnostics, Mannheim, Germany), and the glomerular filtration rate (eGFR) was estimated using the Modification of Diet in Renal disease (MDRD) formula (Instructions for use, Cobas c 702 analyzer). The serum level of NT-proBNP, used as a reference biomarker, was measured using a proBNP II STAT assay on a Cobas e 602 analyzer (Roche Diagnostics). The limit of detection (LoD) for this assay was 5 pg/mL (proBNP II STAT, Cobas®, © 2014, Roche Diagnostics).
Ansari U., Behnes M., Hoffmann J., Natale M., Fastner C., El-Battrawy I., Rusnak J., Kim S.H., Lang S., Hoffmann U., Bertsch T., Borggrefe M, & Akin I. (2018). Galectin-3 Reflects the Echocardiographic Grades of Left Ventricular Diastolic Dysfunction. Annals of Laboratory Medicine, 38(4), 306-315.
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6

Biomarker Quantification in Serum Samples

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All expressed biomarkers were measured in the serum of patients' blood. All samples were obtained by venipuncture into serum monovettes Õ and centrifuged at 2500 g for 10 min at 20 C. The aliquoted samples were cooled down with liquid nitrogen before being stored at À80 C until analysis. The complete processing was conducted within 2 h after blood extraction. After thawing, the samples were mixed gently by inverting and centrifuged with 2500 g for 10 min at 20 C, respectively, 3000 g for 30 min for hsTnI at 4 C.
HsTnT was measured with the Troponin T hs STAT assay on a cobas e 602 analyzer (Roche Diagnostics, Mannheim, Germany). The limit of blank (LoB) for this assay was 3 ng/L and the limit of detection (LoD) was 5 ng/L as described in the instructions for use. 29 HsTnI was measured with the STAT High sensitivity Troponin-I assay on an Architect i1000 analyzer (Abbott, Wiesbaden, Germany). The limit of blank (LoB) was 0.7-1.3 ng/L for this assay as described in the instructions for use. 30 NT-proBNP was measured with the proBNP II STAT assay on a cobas e 602 analyzer (Roche Diagnostics, Mannheim, Germany). The LoD for this assay was 5 ng/L. 31 Creatinine was measured with the Creatinine Jaffe Gen.2 assay on a cobas c 702 analyzer (Roche Diagnostics, Mannheim, Germany).
Natale M., Behnes M., Kim S.H., Hoffmann J., Reckord N., Hoffmann U., Budjan J., Lang S., Borggrefe M., Papavassiliu T., Bertsch T, & Akin I. (2018). High sensitivity troponin T and I reflect left atrial function being assessed by cardiac magnetic resonance imaging. Annals of clinical biochemistry, 55(2).
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7

Biomarker Profiling of Metabolic Health

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Blood samples were collected in a fasting state for measurement of albumin, total cholesterol, triglycerides, low-density lipoprotein (LDL), high-density lipoprotein (HDL), high sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), soluble intercellular adhesion molecule-1 (sICAM-1), tumor-necrosis-factor-α (TNF-α), and hemoglobin A1C (Hgb A1C). Plasma was assayed for albumin, total cholesterol, triglycerides, LDL, HDL, hsCRP, Hgb A1C on the same day as collection by the SF VAMC lab per standard methodology (Beckman Coulter Analyzer). The coefficient of variation for hsCRP using this procedure is 5.1%. Serum was isolated at the same time points for homocysteine and assayed the same day as collection by the SF VAMC lab per standard methodology (Abbott Diagnostics Architect i1000 Analyzer, Lake Forest, IL). Serum was processed per standard protocol and stored at −80°C until assayed for IL-6, sICAM-1, and TNF-α per standard kit protocol (R&D Systems Inc., Minneapolis, MN). The typical coefficients of variation for IL-6, sICAM-1, and TNF-α are 7.4%, 4.6%, and 5.4%, respectively. The lower limits of detection are 0.04pg/ml, 0.1ng/ml, and 0.11pg/ml, respectively.
Chong K.C., Owens C.D., Park M., Alley H.F., Boscardin W.J., Conte M.S., Gasper W.J, & Grenon S.M. (2014). Relationship between Kidney Disease and Endothelial Function in Peripheral Artery Disease. Journal of vascular surgery, 60(6), 1605-1611.
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8

Quantitative Hepatitis B Serology

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The Architect i1000 analyzer (Abbott Diagnostics, Abbott Park, IL, USA), which uses a chemiluminescent microparticle immunoassay (CMIA), was used to analyze HBsAg, HBeAg, and anti-HBe antibodies. Serum HBsAg was determined quantitatively, whereas serum HBeAg and anti-HBe were determined qualitatively. Analysis of these serological markers was conducted according to the manufacturer’s protocol.
Gozlan Y., Aaron D., Davidov Y., Likhter M., Ben Yakov G., Cohen-Ezra O., Picard O., Erster O., Mendelson E., Ben-Ari Z., Abu Baker F, & Mor O. (2022). HBV-RNA, Quantitative HBsAg, Levels of HBV in Peripheral Lymphocytes and HBV Mutation Profiles in Chronic Hepatitis B. Viruses, 14(3), 584.
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9

Comprehensive Metabolic Profiling of Fasting Subjects

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Blood samples were collected from subjects in a fasting state for measurement of creatinine, estimated glomerular filtration rate, albumin, hemoglobin A1C as well as total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein. Plasma was assayed for these analytes the same day as collection by the SFVAMC lab per standard methodology (Beckman Coulter Analyzer). Serum was isolated at the same time points for homocysteine and assayed the same day as collection by the SFVAMC lab per standard methodology (Abbott Diagnostics Architect i1000 Analyzer, Lake Forest, IL).
Grenon S.M., Owens C.D., Nosova E.V., Hughes-Fulford M., Alley H.F., Chong K., Perez S., Yen P.K., Boscardin J., Hellmann J., Spite M, & Conte M.S. (2015). Short-Term, High-Dose Fish Oil Supplementation Increases the Production of Omega-3 Fatty Acid–Derived Mediators in Patients With Peripheral Artery Disease (the OMEGA-PAD I Trial). Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 4(8), e002034.
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