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Percp ef710

Manufactured by Thermo Fisher Scientific
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PerCP-eF710- is a fluorescent dye used in flow cytometry applications. It is a tandem dye that can be excited by a 488nm laser and emits in the 710-750nm range. The dye is conjugated to proteins or other molecules to label and detect them in flow cytometry experiments.

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Lab products found in correlation

Lipopolysaccharides (lps), by Thermo Fisher Scientific (1 mentions) Celltrace violet ctv, by Thermo Fisher Scientific (1 mentions) Cd43 negative selection, by Miltenyi Biotec (1 mentions) Lipopolysaccharides (lps), by R&D Systems (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Facsaria cell sorter, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Interleukin 4 (il 4), by R&D Systems (1 mentions) Tgf β, by R&D Systems (1 mentions) Brilliant violet 421, by Thermo Fisher Scientific (1 mentions) Percp cy5, by Thermo Fisher Scientific (1 mentions) Apcef780, by Thermo Fisher Scientific (1 mentions) Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (1 mentions) Pe cy7, by Thermo Fisher Scientific (1 mentions)

3 protocols using percp ef710

1

Monoclonal Antibodies for Immune Cell Phenotyping

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FITC-labeled mAbs specific for human CD11a (MEM-25) and CD11c (BU15), for mouse CD11a (M17/4) and CD11b (M1/70) and FITC-labeled rat IgG2b (RTG2B1-2) isotype control were purchased from Exbio (Vestec, Czech Republic). OKM1 mAb recognizing human CD11b was isolated from the OKM1 hybridoma purchased from the European Collection of Cell Cultures, Porton Down, UK. PerCP-eF710-labeled mAb specific for mouse CD11a (M17/4) and PerCP-eF710- and FITC-labeled rat IgG2a (eBR2a) isotype controls were obtained from eBioscience (San Diego, CA, USA).
Rahman W.U., Osickova A., Klimova N., Lora J., Balashova N, & Osicka R. (2020). Binding of Kingella kingae RtxA Toxin Depends on Cell Surface Oligosaccharides, but Not on β2 Integrins. International Journal of Molecular Sciences, 21(23), 9092.
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2

Ex Vivo Murine B Cell Differentiation

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For ex vivo assays, naïve splenic B cells were purified by CD43 negative selection (Miltenyi Biotec), cultured at a density of 106 per ml, and stimulated with either LPS (30 μg/ml, Sigma), LPS (30 μg/ml) + IL-4 (12.5ng/ml, R&D Systems), or LPS (10 μg/ml) + TGF-β (2ng/ml, R&D Systems) + anti-IgD dextran conjugates (300 ng/ml, Fina BioSolutions) for CSR to IgG3, IgG1, or IgA, respectively. For proliferation assays, naïve splenic B cells were labeled with 5 μM CellTrace Violet (CTV, Thermo Fisher Scientific) according to manufacturer’s protocol, activated with LPS, LPS+IL-4 or LPS+TGFβ+ anti-IgD dextran and dye dilution tracked from d0 to d4. Antibodies for flow cytometry were as follows: B220 (BV510, FITC, PerCPCy5.5; clone RA3–6B2), IgA (PE; clone mA-6E1), IgG1 (BV510, APC; clone X56), IgG3 (FITC; clone R40–82), CD69 (PE/Cy7; clone H1.2F3), CD86 (AF700; clone GL-1), MHC Class II I-Ab (eFLuor450; clone AF6–120.2), Fas (BV510; clone Jo2), GL7 (FITC, PerCP-eF710; clone GL7) and Zombie Red fixable viability dye; all were purchased from eBioscience, BD, and BioLegend. Samples were analyzed on an LSR II flow cytometer (BD). Cell sorting was carried out in a FACSAria cell sorter (BD). All data analysis was performed using FlowJo software (version 9.9; Tree Star).
Nicolas L., Cols M., Smolkin R., Fernandez K.C., Yewdell W.T., Yen W.F., Zha S., Vuong B.Q, & Chaudhuri J. (2019). ATM influences germinal center integrity. Journal of immunology (Baltimore, Md. : 1950), 202(11), 3137-3142.
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3

Murine Natural Killer Cell Identification

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Cells were stained with fluorochrome- or biotin-labeled antibodies for 30 min on ice. The following antibodies conjugated to FITC, PE, PerCp-Cy5.5, PerCP-ef710, PeCy7, APC, APC-ef780, Pacific Blue, or Brilliant Violet 421 were purchased from eBiosciences or BD Biosciences: CD3ε, CD4, CD8α, CD19, Ter119, CD122, NK1.1, NKp46, DX5, CD69, CD11b, CD25, Klrg1, IL7Rα, Sca1, cKit, CD43, B220, and Gr1. Propidium iodide was used to exclude dead cells. Cells were acquired on a FACS Canto, LSRII, or Fortessa or sorted with a FACS ARIAII and analyzed with FLOWjo. Through this study mNK cells were identified using a lineage cocktail containing CD19, CD3, CD4, CD8, and Ter119 antibodies and propidium iodide to exclude dead cells. NK cells were either Lin-CD122+NK1.1+ or Lin-CD122+NKp46/NK1.1+ and DX5+. NKP were defined as Lin-CD122+NK1.1-.
Xu Y., Evaristo C., Alegre M.L., Gurbuxani S, & Kee B.L. (2015). Analysis of GzmbCre as a Model System for Gene Deletion in the Natural Killer Cell Lineage. PLoS ONE, 10(4), e0125211.
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