Mch100105

Manufactured by Merck Group

The MCH100105 is a laboratory equipment product from Merck Group. It is a device used for performing various laboratory tasks and experiments. The core function of this product is to provide a controlled and precise environment for conducting scientific research and analysis. No further details are available for this specific product.

Automatically generated - may contain errors

Lab products found in correlation

Mch100106, by Merck Group (2 mentions) Bcl 2 activation dual detection assay, by Merck Group (1 mentions) Muse cell analyzer, by Merck Group (1 mentions)

2 protocols using mch100105

Flow Cytometric Analysis of Casticin-Treated Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The flow cytometric analyses used in this study are Annexin V & Dead Cell assay, Multicaspase assay, Bcl-2 Activation Dual Detection assay, and Cell Cycle Analysis (MCH100105, MCH100109, MCH200105, and MCH100106) from EMD Millipore. Assays were performed as per protocols published earlier [19 (link)]. Cells were plated at 2 × 10⁵ cells per well in triplicate in 12-well culture plates. Control groups received the growth media, and for the treatment group, 5 μM of casticin in growth media was added. After 48 h of incubation, the cytometric analyses were performed with a Muse cell analyzer as per the manufacturer’s instructions.

Kowalski M., Assa A., Patil K., Terrell C., Holliday N, & Pai S.B. (2022). Casticin Impacts Key Signaling Pathways in Colorectal Cancer Cells Leading to Cell Death with Therapeutic Implications. Genes, 13(5), 815.

+ Open protocol

+ Expand

Cell Cycle and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA content (cell cycle), and annexin V/7-AAD expression (apoptosis) were established with a Muse cell analyzer (EMD Millipore) and dedicated kits (EMD Millipore, MCH100106 and MCH100105).

Langhendries J.L., Nicolas E., Doumont G., Goldman S, & Lafontaine D.L. (2016). The human box C/D snoRNAs U3 and U8 are required for pre-rRNA processing and tumorigenesis. Oncotarget, 7(37), 59519-59534.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!