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Pp1 antibody

Manufactured by Santa Cruz Biotechnology
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The PP1 antibody is a reagent used in research applications for the identification and detection of the protein phosphatase 1 (PP1) enzyme. PP1 is a serine/threonine-specific protein phosphatase that plays a crucial role in various cellular processes by regulating the phosphorylation state of its target proteins.

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Lab products found in correlation

Transferrin receptor, by Thermo Fisher Scientific (2 mentions)

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Anti-PP1 antibody (3 citations)

2 protocols using pp1 antibody

1

Assessing GluN2B Phosphorylation and Synaptic Levels

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Data from acute slices treated with either NMDA or Cal A were pooled. A Spearman’s rank correlation was calculated to verify association between normalized GluN2B-pS1480 levels and normalized synaptic GluN2B content. Subsequently, the data were fit to an exponential model following the form y = eax + b. Model was obtained by regressing the natural log of synaptic GluN2B content against GluN2B-pS1480 levels using the Im function in R. F-test was used to calculate statistical significance of the model.
Our antibody against phosphorylation state-specific GluN2B S1480 (Ac-CGHVYEKLSSIE(pS)DV-OH) was generated by New England Peptide. Antibodies against GluN2B and PSD-95 were obtained from Neuromab. transferrin receptor, CaMKII, and PP1γ were obtained from Thermo Fisher. PP1 antibody was obtained from Santa Cruz. All drugs and inhibitors were obtained from Tocris Biosciences.
Chiu A.M., Wang J., Fiske M.P., Hubalkova P., Barse L., Gray J.A, & Sanz-Clemente A. (2019). NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content. Cell reports, 28(2), 332-341.e5.
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2

Assessing GluN2B Phosphorylation and Synaptic Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Data from acute slices treated with either NMDA or Cal A were pooled. A Spearman’s rank correlation was calculated to verify association between normalized GluN2B-pS1480 levels and normalized synaptic GluN2B content. Subsequently, the data were fit to an exponential model following the form y = eax + b. Model was obtained by regressing the natural log of synaptic GluN2B content against GluN2B-pS1480 levels using the Im function in R. F-test was used to calculate statistical significance of the model.
Our antibody against phosphorylation state-specific GluN2B S1480 (Ac-CGHVYEKLSSIE(pS)DV-OH) was generated by New England Peptide. Antibodies against GluN2B and PSD-95 were obtained from Neuromab. transferrin receptor, CaMKII, and PP1γ were obtained from Thermo Fisher. PP1 antibody was obtained from Santa Cruz. All drugs and inhibitors were obtained from Tocris Biosciences.
Chiu A.M., Wang J., Fiske M.P., Hubalkova P., Barse L., Gray J.A, & Sanz-Clemente A. (2019). NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content. Cell reports, 28(2), 332-341.e5.
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