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Atn 224

Manufactured by Cayman Chemical
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ATN-224 is a lab equipment product offered by Cayman Chemical. It is a device used for the analysis and measurement of various chemical and biological samples. The core function of ATN-224 is to provide accurate and reliable data for research and analytical purposes.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (4 mentions) Ultrapure water, by Thermo Fisher Scientific (2 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions) Jc 1 dye, by Thermo Fisher Scientific (2 mentions) Xenolight d luciferin, by PerkinElmer (2 mentions) Sterile 0.9 saline solution, by Teknova (2 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (2 mentions) 1 2 dipalmitoyl sn glycero 3 phosphoethanolamine n methoxy polyethyleneglycol 2000 ammonium salt dppe peg2000, by Avanti Polar Lipids (2 mentions) 2 methyl 6 phenyl 3 7 dihydroimidazo 1 2 a pyrazin 3 one, by Tokyo Chemical Industry (2 mentions) Rapamycin, by Enzo Life Sciences (1 mentions) Mg132, by Enzo Life Sciences (1 mentions) Etomoxir, by Merck Group (1 mentions) Menadione, by Merck Group (1 mentions) Oligomycin, by Merck Group (1 mentions) Pf 06424439, by Merck Group (1 mentions) Ebselen, by Bio-Techne (1 mentions) Ab54037, by Abcam (1 mentions) Azd 3988, by Bio-Techne (1 mentions) Tempol, by Merck Group (1 mentions) Gfp 2956, by Cell Signaling Technology (1 mentions)

5 protocols using atn 224

1

Fluorescent Probes for Cell Imaging

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All chemicals were obtained from Sigma-Aldrich unless otherwise stated. 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (ammonium salt)(DPPE-PEG2000) was purchased from Avanti Polar Lipids. fSP (Poly[(9,9-dioctyl-2,7-divinylenefluorenylene)-alt-{2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylene}]) was purchased from Luminescence Technology Corp. 2-Methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (CLA) was purchased from TCI America. JC-1 dye was purchased from Invitrogen. ATN224 was purchased from Cayman Chemical Company. XenoLight™ D-Luciferin was purchased from Perkin Elmer. TRIzol® reagent was purchase from Thermo Fisher Scientific. Ultrapure water was purchased from Invitrogen. 1× PBS, 1× D-PBS, and 1× HBSS were purchased from Gibco. Sterile 0.9 % saline solution was purchased from TEKnova. CCL-1 was synthesized with the procedures reported previously.58 (link)
Cui L., Gouw A.M., LaGory E.L., Guo S., Attarwala N., Tang Y., Qi J., Chen Y.S., Gao Z., Casey K.M., Bazhin A.A., Chen M., Hu L., Xie J., Fang M., Zhang C., Zhu Q., Wang Z., Giaccia A.J., Gambhir S.S., Zhu W., Felsher D.W., Pegram M.D., Goun E.A., Le A, & Rao J. (2020). ED SUM: Triple-negative breast cancer is inhibited by depleting mitochondrial copper in mice.: Mitochondrial copper depletion suppresses triple-negative breast cancer in mice. Nature biotechnology, 39(3), 357-367.
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2

Fluorescent Probes for Cell Imaging

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All chemicals were obtained from Sigma-Aldrich unless otherwise stated. 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (ammonium salt)(DPPE-PEG2000) was purchased from Avanti Polar Lipids. fSP (Poly[(9,9-dioctyl-2,7-divinylenefluorenylene)-alt-{2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylene}]) was purchased from Luminescence Technology Corp. 2-Methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (CLA) was purchased from TCI America. JC-1 dye was purchased from Invitrogen. ATN224 was purchased from Cayman Chemical Company. XenoLight™ D-Luciferin was purchased from Perkin Elmer. TRIzol® reagent was purchase from Thermo Fisher Scientific. Ultrapure water was purchased from Invitrogen. 1× PBS, 1× D-PBS, and 1× HBSS were purchased from Gibco. Sterile 0.9 % saline solution was purchased from TEKnova. CCL-1 was synthesized with the procedures reported previously.58 (link)
Cui L., Gouw A.M., LaGory E.L., Guo S., Attarwala N., Tang Y., Qi J., Chen Y.S., Gao Z., Casey K.M., Bazhin A.A., Chen M., Hu L., Xie J., Fang M., Zhang C., Zhu Q., Wang Z., Giaccia A.J., Gambhir S.S., Zhu W., Felsher D.W., Pegram M.D., Goun E.A., Le A, & Rao J. (2020). ED SUM: Triple-negative breast cancer is inhibited by depleting mitochondrial copper in mice.: Mitochondrial copper depletion suppresses triple-negative breast cancer in mice. Nature biotechnology, 39(3), 357-367.
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3

ATN-224 Oral Gavage Protocol

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Animals were treated with ATN-224 (Cayman chemical #23553) in sterile saline at the dose of 4.5 mg/kg/day by oral gavage. The treatment was performed on days 0, 1, and 2 immediately prior to the SMP application. Vehicle group received saline.
Biyashev D., Siwicka Z.E., Onay U.V., Demczuk M., Xu D., Ernst M.K., Evans S.T., Nguyen C.V., Son F.A., Paul N.K., McCallum N.C., Farha O.K., Miller S.D., Gianneschi N.C, & Lu K.Q. (2023). Topical application of synthetic melanin promotes tissue repair. NPJ Regenerative Medicine, 8, 61.
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4

Neurodegeneration Modeling in LUHMES Cells

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Lund human mesencephalic (LUHMES) cells were grown as published [18 (link)]. Four days post differentiation, cells were treated with small molecules in fresh media: MPP+ (10 µM, 48 h), PHT (20 nM, 48 h), rotenone (10 nM, 48 h), carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) (1 mM, 48 h, Tocris), paraquat (100 µM, 48 h), BafA1 (500 nM, 4 h, Toronto research chemicals), rapamycin (500 nM, 4 h Enzo Lifesciences), MG132 (10 µM, 24 h, Enzo Lifesciences), ATN-224 (10 µM, 6 h, Cayman chemicals). Starvation occurred with EBSS for 4 h.
Baeken M.W., Schwarz M., Kern A., Moosmann B., Hajieva P, & Behl C. (2021). The selective degradation of sirtuins via macroautophagy in the MPP+ model of Parkinson’s disease is promoted by conserved oxidation sites. Cell Death Discovery, 7, 286.
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5

Mitochondrial Stress Response Modulators

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Compounds were used at the following concentrations unless otherwise noted: 50 μM AZD3988 (Tocris), 30 μM A922500 (Stratech), 50 μM AZD7687 (Stratech), 70 μM T863 (Sigma), 1 μM Oligomycin (Sigma), 0.5 μM FCCP (Sigma), 1 μM Antimycin-A (Sigma), 1 μM Rotenone (Sigma), 50 μM PF-06424439 (Sigma), 5 μM Ebselen (Tocris), 1 mM Tempol, 200 μM Paraquat (Sigma), Menadione (Sigma), 100 μM Etomoxir (Sigma), 2 μM Ferrostatin-1 (Sigma), 5 μM ML385 (Selleckchem), and 1 μM ATN-224 (Cayman Chemical). Antibodies against DGAT1 (ab54037) and 4-Hydroxynonenal (ab46545) were purchased from abcam. Antibodies against Vinculin (66305-1-Ig), Beta-Tubulin (10094-1-AP), PINK1 (23274-1-AP), Parkin (14060-1-AP), SOD1 (10269-1-AP), SOD2 (24127-1-AP), SESN2 (10795-1-AP) and GAPDH (60004-1-Ig) were purchased from Proteintech. Antibodies against phospho-AMPK (50081), phospho-RAPTOR (2083), Caspase-3 (9662), phospho-ERK (4370) and GFP (2956) were purchased from Cell Signalling. The antibody against gamma-tubulin (T5326) was purchased from Sigma. The antibody against ERK2 (C-14) was from Santa Cruz Biotechnology.
Wilcock D.J., Badrock A.P., Wong C.W., Owen R., Guerin M., Southam A.D., Johnston H., Telfer B.A., Fullwood P., Watson J., Ferguson H., Ferguson J., Lloyd G.R., Jankevics A., Dunn W.B., Wellbrock C., Lorigan P., Ceol C., Francavilla C., Smith M.P, & Hurlstone A.F. (2022). Oxidative stress from DGAT1 oncoprotein inhibition in melanoma suppresses tumor growth when ROS defenses are also breached. Cell Reports, 39(12), 110995.
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