Anti dusp4

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-DUSP4 is a primary antibody that specifically recognizes the Dual Specificity Phosphatase 4 (DUSP4) protein. DUSP4 is a member of the dual-specificity protein phosphatase family that acts as a negative regulator of mitogen-activated protein kinases.

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DUSP4

2 protocols using anti dusp4

Immunoblot Analysis of MAPK Pathway

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A375‐Cas9, IGR1‐Cas9, and WM983B‐Cas9 cells were transduced with lentiviral sgRNA expression vectors and cell lysates were prepared for immunoblot analysis as described previously (Christodoulou et al., 2019). Antibodies used for detection included anti‐DUSP4 (1:1,000), anti‐PPP2R2A (1:1,000), anti‐BRAF (1:1,000) (Cell Signaling Technology), and anti‐Vinculin (1:1,000, clone hVIN‐1, Sigma‐Aldrich) as a loading control. Secondary anti‐mouse and anti‐rabbit antibodies were used at 1:10,000 (Jackson ImmunoResearch Europe).

Christodoulou E., Rashid M., Pacini C., Droop A., Robertson H., van Groningen T., Teunisse A.F., Iorio F., Jochemsen A.G., Adams D.J, & van Doorn R. (2020). Analysis of CRISPR‐Cas9 screens identifies genetic dependencies in melanoma. Pigment Cell & Melanoma Research, 34(1), 122-131.

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Signaling Pathway Protein Analysis

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Cells were washed with ice-cold phosphate-buffered saline (PBS) and then solubilized with protease inhibitor (Upstate Biotechnology, Temesula, CA, USA)-containing RIPA buffer. Lysates were immunoblotted with indicated antibodies, anti-G9a, anti-DUSP4, anti-p-AMPK, anti-AMPK, anti-S6K and anti-p-S6K (T389) (Cell Signaling Technology), anti-H3K9me1 (Millipore, MA, USA), anti-GLP, anti-H3K9me2, anti-histone 3, anti-LC3B (Abcam), anti-H3K9me3, anti-caspase 3, anti-PARP, anti-p62, anti-β-actin anti-GAPDH, anti-α-tubulin (GeneTex), anti-p-Akt, anti-Akt, anti-p-ERK and anti-ERK (Santa Cruz Biotechnology, Santa Cruz, CA).

Li K.C., Hua K.T., Lin Y.S., Su C.Y., Ko J.Y., Hsiao M., Kuo M.L, & Tan C.T. (2014). Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma. Molecular Cancer, 13, 172.

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