Plant mda assay kit

Manufactured by Nanjing Jiancheng

Sourced in China

The Plant MDA Assay Kit is a laboratory tool designed to measure the level of malondialdehyde (MDA) in plant samples. MDA is an important biomarker that indicates oxidative stress in plants. The kit provides a standardized method for quantifying MDA concentration, which can be used to assess plant health and responses to various environmental factors.

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Lab products found in correlation

H2o2 assay kit, by Nanjing Jiancheng (3 mentions) A064 1 1, by Nanjing Jiancheng (2 mentions) A084 3 1, by Nanjing Jiancheng (2 mentions) A001 1 2, by Nanjing Jiancheng (2 mentions) A007 1 1, by Nanjing Jiancheng (2 mentions) Total sod assay kit, by Nanjing Jiancheng (2 mentions) Peroxidase assay kit, by Nanjing Jiancheng (1 mentions) Pod assay kit, by Nanjing Jiancheng (1 mentions) Cat assay kit, by Nanjing Jiancheng (1 mentions)

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MDA kit

6 protocols using plant mda assay kit

Measuring Antioxidant Capacity in Plant Tissues

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A certain weight of leaf or shoot sample was crushed in a prechilled pestle and crushed with a mortar in an appropriate volume of prechilled extraction buffer. After uniform homogenization and centrifugation at 4,000 g for 15 min at 4°C, the supernatant was collected and used for biochemical analyses.
Antioxidative capabilities were estimated by quantifying peroxidation products and antioxidase activity levels. The malondialdehyde (MDA) content was determined using a Plant MDA Assay Kit (TBA method; A003-3-1, Nanjing Jiancheng, Nanjing, China). The hydrogen peroxide (H 2 O 2 ) level was determined using an H 2 O 2 Assay Kit (H 2 O 2 reacted with titanium salt and is then quantified at OD 415 ; A064-1-1, Nanjing Jiancheng), and the superoxide anion (O 2 -) level was determined using an O 2 -Assay Kit (sulfanilamide method; R30342, Shanghai Yuanye, Shanghai, China). The activity levels of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were determined using the Total SOD (WST-1 method; A001-1-2, Nanjing Jiancheng), POD (H 2 O 2 catalysis method; A084-3-1, Nanjing Jiancheng) and CAT (H 2 O 2 catalysis method; A007-1-1, Nanjing Jiancheng) Assay Kits, respectively, following the manufacturer's instructions. All the leaf and shoot samples were measured three times in parallel.

Sun H., Wu S., & Wu L. (2021). White oak (Quercus fabri Hance) regenerated stump sprouts show few senescence symptoms during 40 years’ growth in a natural forest.

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Antioxidant Capacity Analysis of Plant Extracts

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A certain weight of leaf or shoot sample was crushed in a prechilled pestle and crushed with a mortar in an appropriate volume of prechilled extraction buffer. After uniform homogenization and centrifugation at 4,000 g for 15 min at 4°C, the supernatant was collected and used for biochemical analyses.
Antioxidative capabilities were estimated by quantifying peroxidation products and antioxidase activity levels.
The malondialdehyde (MDA) content was determined using a Plant MDA Assay Kit (TBA method; A003-3-1, Nanjing Jiancheng, Nanjing, China). The hydrogen peroxide (H 2 O 2 ) level was determined using an H 2 O 2 Assay Kit (H 2 O 2 reacted with titanium salt and is then quanti ed at OD 415 ; A064-1-1, Nanjing Jiancheng), and the superoxide anion (O 2 -) level was determined using an O 2 -Assay Kit (sulfanilamide method; R30342, Shanghai Yuanye, Shanghai, China). The activity levels of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were determined using the Total SOD (WST-1 method; A001-1-2, Nanjing Jiancheng), POD (H 2 O 2 catalysis method; A084-3-1, Nanjing Jiancheng) and CAT (H 2 O 2 catalysis method; A007-1-1, Nanjing Jiancheng) Assay Kits, respectively, following the manufacturer's instructions. All the leaf and shoot samples were measured three times in parallel.

Sun H., Wu S., & Wu L. (2020). White Oak (Quercus fabri Hance) regenerated stump sprouts show few symptom of senescence during 40 years’ growth in the natural forest.

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Electrolyte, Antioxidant and Chlorophyll Assay

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The relative electrolyte conductivity (REC) and proline content were determined according to the method of Geng et al. (2019) (link). Malondialdehyde (MDA) and superoxide dismutase (SOD) were determined in the samples using plant MDA assay kit and total SOD assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), respectively, following the manufacturer’s instructions. Chlorophyll content was determined following the method described by Brini et al. (2009) (link). Statistical calculations were performed using SPSS 17.0, and Duncan’s test was used for significance analysis (P < 0.05, significant; P < 0.01, highly significant).

Zhang J., Liang L., Xie Y., Zhao Z., Su L., Tang Y., Sun B., Lai Y, & Li H. (2022). Transcriptome and Metabolome Analyses Reveal Molecular Responses of Two Pepper (Capsicum annuum L.) Cultivars to Cold Stress. Frontiers in Plant Science, 13, 819630.

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Antioxidant Enzymes and MDA Determination

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The REC was measured using the immersion method [57 (link)]. Pro content was assayed following the methods described by Bates et al. [58 (link)]. Briefly, 0.5 g leaf samples were added in 5 mL of 3% sulfosalicylic acid and then placed in boiling water for 10 min. After filtering, the supernatant (2 mL) was mixed with 2 mL of acetic acid glacial and 2 mL of ninhydrin. Subsequently, the mixture was maintained in boiling water for 30 min. After cooling, 4 mL of methylbenzene was added. Absorbance was measured at 520 nm using methylbenzene as blank. MDA content was determined using a plant MDA assay kit (Nanjing Jiancheng, Nanjing, China) in accordance with the manufacturer’s instructions. The activities of antioxidant enzymes (SOD, POD, and CAT) were determined following previously described procedures [48 (link)].

Zhang J., Xie M., Yu G., Wang D., Xu Z., Liang L., Xiao J., Xie Y., Tang Y., Sun G., Sun B., Huang Z., Lai Y, & Li H. (2023). CaSPDS, a Spermidine Synthase Gene from Pepper (Capsicum annuum L.), Plays an Important Role in Response to Cold Stress. International Journal of Molecular Sciences, 24(5), 5013.

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Measuring Plant Stress Responses

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The first young leaf on the top of each of the three plants was collected at each time point (10 °C for 0, 3, 5, and 7 days and recovery after cold stress for 2 days) for each biological replicate. The MDA content was determined by the plant MDA assay kit (Nanjing Jiancheng Bio, Nanjing, China), and the POD activity was tested by the peroxidase assay kit (Nanjing Jiancheng Bio, Nanjing, China). Five replicates were performed for each sample. Three independent experiments were performed as biological replicates.

Gao J., Dou T., He W., Sheng O., Bi F., Deng G., Gao H., Dong T., Li C., Zhang S., Yi G., Hu C, & Yang Q. (2021). MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana. BMC Plant Biology, 21, 97.

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Profiling Antioxidant Defense in Plants

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A certain weight of leaf or shoot sample was crushed in a prechilled pestle and mortar using an appropriate volume of prechilled extraction buffer. After uniform homogenization and centrifugation at 13,000 rpm for 15 min at 4 °C, the supernatant was collected and used for biochemical analyses.
Antioxidative abilities were estimated by quantifying peroxidation products and antioxidase activities.
Malondialdehyde (MDA) was determined by Plant MDA Assay Kit (TBA method; A003-3-1, Nanjing Jiancheng, China), hydrogen peroxide (H 2 O 2 ) was determined by H 2 O 2 Assay Kit (H 2 O 2 react with titanium salt and quanti ed under OD415; A064-1-1, Nanjing Jiancheng, China), superoxide anion (O 2 -) was determined by O 2 -Assay Kit (sulfanilamide method; R30342, Shanghai Yuanye, China), superoxide dismutase (SOD) activity was determined by Total SOD Assay Kit (WST-1 method; A001-1-2, Nanjing Jiancheng, China), peroxidase (POD) activity was determined by POD Assay Kit (H 2 O 2 catalysis method; A084-3-1, Nanjing Jiancheng, China), and catalase (CAT) activity was determined by CAT Assay Kit (H 2 O 2 catalysis method; A007-1-1, Nanjing Jiancheng, China) as described by manufacturers. All leaf and shoot samples were measured three times parallelly.

Sun H., Wu S., & Wu L. (2020). White Oak (Quercus Fabri Hance) Regenerated Stump Sprouts Show Few Symptom of Senescence During 40 Years’growth in the Natural Forest.

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